Detection of the BCR-ABL Gene by Interphase Fluorescence In Situ Hybridization (iFISH) in Chronic Myelogenous Leukemia Patients after Hemopoietic Stem Cell Transplantation: The Feasibility of iFISH Monitoring of Therapeutic Response in Peripheral Blood
- 92 Downloads
The detection of the Philadelphia (Ph) translocation has been accomplished primarily by cytogenetic analysis and reverse transcriptase polymerase chain reaction (RT-PCR). RT-PCR is highly sensitive (1/104-106) but not quantitatively reliable and is thus unsuitable for the monitoring of Ph-positive cells during therapy. Interphase fluorescence in situ hybridization (iFISH) allows analysis of a large number of cells (>500) in a timely and efficiently quantitative manner. We obtained 118 peripheral blood (PB) and 127 bone marrow (BM) samples from 75 adult chronic myelogenous leukemia (CML) patients undergoing stem cell transplantation. We simultaneously performed nested RT-PCR and iFISH for all samples. False-positive cells were detected in 2.48% ± 0.93% (mean ± SD) of PB samples and 2.75% ± 0.83% of BM samples. The iFISH results for PB and BM ranged from 1.4% to 92.8% and 1.0% to 93.8%, respectively. Correlation analysis of iFISH results for PB versus BM samples showed a strong relation (r = .993). A significant correlation (P < .05) was also found between iFISH and first-round RT-PCR. The sensitivity of BCR-ABL iFISH was similar to that of first-round RT-PCR, and iFISH results for PB and BM were also well correlated. Thus, iFISH analysis of PB and/or BM samples may be more clinically reliable than RT-PCR in the quantitative monitoring of BCR-ABL fusion in CML after transplantation.
Key wordsChronic myelogenous leukemia Interphase FISH BCR-ABL Hemopoietic stem cell transplantation
Unable to display preview. Download preview PDF.
- 1.Nowell PC, Hungerford DA. A minute chromosome in human chronic myelocytic leukemia.Science. 1960;132:1497–1501.Google Scholar
- 21.Garcia-Isidoro M, Tabernero MD, Garcia JL, et al. Detection of the Mbcr/abl translocation in chronic myeloid leukemia by fluorescence in situ hybridization: comparison with conventional cytogenetics and implications for minimal residual disease detection.Hum Pathol. 1997;28:154–159.CrossRefPubMedGoogle Scholar
- 22.Muhlmann J, Thaler J, Hilbe W, et al. Fluorescence in situ hybridization (FISH) on peripheral blood smears for monitoring Philadelphia chromosome-positive chronic myeloid leukemia (CML) during interferon treatment: a new strategy for remission assessment.Genes Chromosomes Cancer. 1998;21:90–100.CrossRefPubMedGoogle Scholar