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Establishment of a Primary Effusion Lymphoma Cell Line (RM-P1) and In Vivo Growth System Using SCID Mice

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Abstract

Primary effusion lymphoma (PEL) is recognized as a unique lymphoma entity, which occurs exclusively in body cavities as a serous lymphomatous effusion without tumor formation or organ infiltration. We established a cell line of B-cell origin from a pericardial effusion of a 63-year-old Japanese PEL patient who did not have human immunodeficiency virus infection. This PEL cell line had human herpesvirus-8 (HHV-8) and Epstein-Barr virus (EBV) infection. We named this cell line RM-P1. This cell line showed complex chromosomal abnormalities that could not be identified by G-banding. However, spectral karyotyping analysis determined the origin and organization of all unidentified chromosomal abnormalities. When inoculated into the peritoneal cavity of 8 severe combined immunodeficiency (SCID) mice depleted of natural killer cells, RM-P1 cells induced solid tumor with ascites in all animals tested. These tumor and ascitic cells had the same immunogenotypic features as those of the original RM-P1. These 2 types of cells were positive for both HHV-8 and EBV as demonstrated using polymerase chain reaction. Fluorescence-activated cell sorting analyses showed that neither tumors nor ascitic cells grown in SCID mice expressed leukocyte function-associated antigen (LFA)-1α (CD11a), LFA-1β (CD18), LFA-2 (CD2), LFA-3 (CD58), intercellular adhesion molecule (ICAM)-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), or leukocyte endothelial adhesion molecule (LECAM)-1 (CD62L), suggesting that these cytoadhesion molecules are not involved in tumor formation of RM-P1 cells in vivo. The establishment of the RM-P1 cell line and the animal model of PEL may provide insights for understanding the relationship between these viruses and PEL and for understand the mechanism for PEL.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, 903-0215, Okinawa, Japan

    Jun-ichi Miyagi, Masato Masuda, Nobuyuki Takasu, Akitoshi Nagasaki & Tetsuharu Shinjyo

  2. Department of Dermatology, and Department of Infectious Disease and Immunology, Okinawa-Asia Research Center of Medical Science, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Hiroshi Uezato

  3. Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Naoki Kakazu

  4. Department of Infectious Disease and Immunology, Okinawa-Asia Research Center of Medical Science, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Yuetsu Tanaka

Authors
  1. Jun-ichi Miyagi
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  2. Masato Masuda
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  3. Nobuyuki Takasu
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  4. Akitoshi Nagasaki
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  5. Tetsuharu Shinjyo
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  7. Naoki Kakazu
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  8. Yuetsu Tanaka
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Correspondence to Jun-ichi Miyagi.

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Miyagi, Ji., Masuda, M., Takasu, N. et al. Establishment of a Primary Effusion Lymphoma Cell Line (RM-P1) and In Vivo Growth System Using SCID Mice. Int J Hematol 76, 165–172 (2002). https://doi.org/10.1007/BF02982580

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  • DOI: https://doi.org/10.1007/BF02982580

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