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Hb Nishinomiya [Leu-Gly-Inserted between Codons 69(E13) and 70(E14) of β: A Novel Unstable Hemoglobin with Reduced Oxygen Affinity Found in a Patient with Spherocytic Hemolysis

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Abstract

Substantially decreased oxygen saturation levels were incidentally detected by pulseoxymetry in a patient with spherocytic hemolysis who was undergoing laparoscopic splenectomy. Molecular analysis revealed that he was carrying hemoglobin (Hb) Nishinomiya, a novel Hb variant [Leu-Gly-inserted between codons 69(E13) and 70(E14) of β]. Amino acid substitutions around positions 70–73(E13–17) of the β chain are likely to change stability and oxygen affinity, as has been demonstrated in several Hb variants including Hb Seattle. The apparent substitution of the amino acid residues in the heme pocket of the β chain explains the decreased stability and oxygen affinity of Hb Nishinomiya.

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Authors and Affiliations

  1. Departments of Anesthesia, Kobe City General Hospital, Minatojima Nakamachi 4-6, Chuo-ku, Kobe, 650-0046, Japan

    Yoshiyuki Naito

  2. Departments of Hematology and Clinical Immunology, Kobe City General Hospital, Minatojima Nakamachi 4-6, Chuo-ku, Kobe, 650-0046, Japan

    Takayuki Takahashi

  3. Department of Internal Medicine, Nishinomiya Municipal Central Hospital, Nishinomiya, Japan

    Tatsuro Matsunashi

  4. Department of Biochemistry, Kawasaki Medical School, Kurashiki, Japan

    Keiko Harano & Teruo Harano

Authors
  1. Yoshiyuki Naito
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  2. Takayuki Takahashi
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  3. Tatsuro Matsunashi
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  4. Keiko Harano
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  5. Teruo Harano
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Correspondence to Yoshiyuki Naito.

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Naito, Y., Takahashi, T., Matsunashi, T. et al. Hb Nishinomiya [Leu-Gly-Inserted between Codons 69(E13) and 70(E14) of β: A Novel Unstable Hemoglobin with Reduced Oxygen Affinity Found in a Patient with Spherocytic Hemolysis. Int J Hematol 76, 146–148 (2002). https://doi.org/10.1007/BF02982576

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  • DOI: https://doi.org/10.1007/BF02982576

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