Summary
1. Macroscopic characteristics of bacterial cultures have been studied as a means of characterizing cells from which they are derived.
2. By single-cell micro-isolation, it has been demonstrated that the occurrence of smooth cells in rough colonies is due to mutation.
3. It has been shown that a rough colony grown at 37° C. has 20 per cent smooth cells and that a rough colony grown at 27° C. has 37 per cent smooth cells.
4. It has been shown that the percentage of smooth cells in a rough colony depends on the incubation temperature, and not on the history of the cell from which it is derived.
5. Further possibilities of this method of study have been suggested.
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References
Henrici, Arthur T. (1928).Morphological Variation and the Rate of Growth of Bacteria. Microbiology Monographs,1. Springfield: Charles C. Thomas.
Deskowitz, M. &Shapiro, A. (1935). “Numerical relations of unstable variant ofSalmonella Aertrycke.”Proc. Soc. exp. Biol., N.Y.,32, 573–7.
Kahn, M. C. (1929). “Developmental cycle of tubercle bacillus as revealed by single-cell studies.”Amer. Rev. Tuberc. 20, 150–200.
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Shapiro, A., Spiegelman, S. & Koster, H. A rapid method for the study of genetics in large populations. Journ. of Genetics 34, 237–245 (1937). https://doi.org/10.1007/BF02982265
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DOI: https://doi.org/10.1007/BF02982265