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International Journal of Hematology

, Volume 75, Issue 4, pp 370–375 | Cite as

Human CD34 Hematopoietic Stem Cells: Basic Features and Clinical Relevance

  • Kiyoshi Ando
Review Article

Abstract

CD34 has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+ SCT) has been performed for tumor purging and for prevention of graft-versus-host disease. Recently, CD34-negative hemato-poietic stem cells (CD34 HSCs) were identified in mice and humans. Xenogeneic chimera engraftment assays with immun-odeficient mice or preimmune fetal sheep resulted in identification of human CD34 HSCs in cord blood, bone marrow, and granulocyte colony-stimulating factor-mobilized peripheral blood, although no significant clonogenic activity was detected in vitro. These characteristics of CD34 HSCs make the assessment of clinical samples difficult. The generation of CD34+ HSCs from CD34 cells in vitro may be a surrogate assay for detecting CD34 HSC activity. This approach was used in recipients of CD34+ SCT and revealed the absence of a CD34 precursor population.The identification of a positive marker in CD34 HSCs and the development of a simpler and more efficient in vivo assay for CD34 HSCs may allow the diagnostic evaluation of human CD34 HSCs in various clinical procedures and diseases.

Key words

CD34 Hematopoietic stem cell Xenogeneic transplantation assay 

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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  1. 1.Department of Hematology and Rheumatology and Research Center for Cell TransplantationTokai University, School of Medicine, BohseidaiIsehara, KanagawaJapan

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