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International Journal of Hematology

, Volume 75, Issue 4, pp 345–349 | Cite as

Mast Cells Derived from Embryonic Stem Cells: A Model System for Studying the Effects of Genetic Manipulations on Mast Cell Development, Phenotype, and Function In Vitro and In Vivo

  • Mindy Tsai
  • See-Ying Tam
  • Jochen Wedemeyer
  • Stephen J. Galli
Progress in Hematology

Abstract

Large quantities of highly enriched populations of mast cells can be generated from mouse embryonic stem (ES) cells using an in vitro differentiation system. These embryonic stem cell-derived mast cells (ESMCs) exhibit many similarities to mouse bone marrow-derived cultured mast cells (BMCMCs), including the abilities to survive and to orchestrate immunologically specific immunoglobulin E (IgE)-dependent reactions in vivo after transplantation into genetically mast cell—deficient KitW/KitW-v mice. Coupled with the current spectrum of techniques for genetically manipulating ES cells, ESMCs represent a unique model system to analyze the effects of specific alterations in gene structure, expression, or function, including embryonic lethal mutations, on mast cell development, phenotype, and function in vitro and in vivo.

Key words

Embryonic stem cells Mast cells In vitro differentiation Gene targeting Mast cell-deficient mice 

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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Mindy Tsai
    • 1
  • See-Ying Tam
    • 1
  • Jochen Wedemeyer
    • 1
  • Stephen J. Galli
    • 1
  1. 1.Department of PathologyStanford University School of MedicineStanfordUSA

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