International Journal of Hematology

, Volume 75, Issue 3, pp 257–260 | Cite as

F-Blast Is a Useful Marker for Differentiating Hypocellular Refractory Anemia From Aplastic Anemia

  • Jong Weon Choi
  • Masahiko Fujino
  • Masafumi Ito
Rapid Communication


Making a morphologic distinction between hypocellular refractory anemia (hypo RA) and aplastic anemia (AA) is difficult. To investigate the significance of hemoglobin F-containing erythroblasts (F-blasts) and p53 expression in making the distinction between hypo RA and acquired AA, we immunohistochemically assessed F-blasts and p53 in bone marrow specimens from 16 patients with hypo RA, 31 patients with acquired AA, and 15 hematologically normal individuals. F-blast production was elevated in 87.5% (14/16) of patients with hypo RA, but in only 3.2% (1/31) of patients with AA (P < .01). p53 was over-expressed in 75.0% (12/16) of hypo RA patients and in 6.4% (2/31) of AA patients (P < .01). The mean contents of F-blasts and p53-positive cells in patients with hypo RA were 6.31% ± 3.27% and 7.54% ± 4.36%, respectively, of all bone marrow cells, which were significantly higher than for patients with AA (0.35% ± 0.46% and 0.58% ± 1.29%, P < .01). In conclusion, a high prevalence of elevated F-blast production is noted in hypo RA, suggesting that the assessment of F-blasts in bone marrow can be used as an additional tool for differentiating hypo RA from acquired AA.

Key words

Hypocellular refractory anemia Aplastic anemia F-blasts p53 


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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Jong Weon Choi
    • 1
    • 2
  • Masahiko Fujino
    • 3
  • Masafumi Ito
    • 1
  1. 1.Department of PathologyNagoya University HospitalNagoyaJapan
  2. 2.Department of Clinical Pathology, College of MedicineInha UniversityInchonKorea
  3. 3.First Department of PathologyNagoya University School of MedicineNagoyaJapan

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