Advertisement

International Journal of Hematology

, Volume 75, Issue 2, pp 161–165 | Cite as

Expression of the Antiapoptosis Gene Survivin in Human Leukemia

  • Ako Mori
  • Hiroshi Wada
  • Yasuo Nishimura
  • Takahiro Okamoto
  • Yoshinobu Takemoto
  • Eizo Kakishita
Progress in Hematology

Abstract

Loss of the inhibition of apoptosis is important in leukemogenesis and may influence the prognosis. Survivin is an inhibitor of apoptosis that shows selective expression during fetal development and in human malignancies. Survivin expression was examined in human leukemias using the reverse transcriptase—polymerase chain reaction. Survivin gene expression was detected in 17 of 31 patients with acute myelocytic leukemia and 11 of 16 patients with acute lymphocytic leukemia but was not identified in normal bone marrow cells. Survivin expression was lower in patients with M3 acute myelocytic leukemia than in patients with other types of acute leukemia. Survivin was not detected in the chronic phase of chronic myelocytic leukemia but was observed in 5 of 7 patients with chronic myelocytic leukemia in blastic crisis. These findings suggest a relationship between survivin gene expression and hematopoietic cell differentiation. In fact, survivin gene expression was down-regulated during the differentiation of HL-60 cells after treatment with dimethyl sulfoxide or all—trans-retinoic acid. Moreover, the disease-free survival rates of patients with survivin expression were lower than in patients without survivin expression. Accordingly, survivin may have a role in leukemogenesis as well as in other malignancies. Detecting survivin may also provide prognostic information.

Key words

Survivin Acute myelocytic leukemia Acute lymphocytic leukemia Chronic myelocytic leukemia Differentiation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Referencess

  1. 1.
    Thompson CB. Apoptosis in the pathogenesis and treatment of disease.Science. 1995;267:1456–1462.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    LaCasse EC, Baird S, Korneluk RG, MacKenzie AE. The inhibitors of apoptosis (IAPs) and their emerging role in cancer.Oncogene. 1998;17:3247–3259.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,survivin, expressed in cancer and lymphoma.Nat Med. 1997;3:917–921.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Adida C, Berrebi D, Peuchmaur M, Reyes-Mugica M, Altieri DC. Anti-apoptosis gene, survivin, and prognosis of neuroblastoma.Lancet. 1998;351:882–883.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Islam A, Kageyama H, Takada N, et al. High expression ofsurvivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma.Oncogene. 2000;19:617–623.CrossRefPubMedGoogle Scholar
  6. 6.
    Wada H, Mizutani S, Nishimura J, et al. Establishment and molecular characterization of a novel leukemic cell line with Philadelphia chromosome expressing p230 BCR/ABL fusion protein.Cancer Res. 1995;55:3192–3196.PubMedPubMedCentralGoogle Scholar
  7. 7.
    Israeli RS, Miller WH Jr, Su SL, et al. Sensitive nested reverse transcription polymerase chain reaction detection of circulating prostatic tumor cells: comparison of prostate-specific membrane antigen and prostate-specific antigen-based assays.Cancer Res. 1994;54:6306–6310.PubMedGoogle Scholar
  8. 8.
    Gerhardt TM, Schmahl GE, Flotho C, Rath AV, Niemeyer CM. Expression of the Evi-1 gene in haemopoietic cells of children with juvenile myelomonocytic leukemia and normal donors.Br J Haematol. 1997;99:882–887.CrossRefPubMedGoogle Scholar
  9. 9.
    Steinman RA, Hoffman B, Iro A, Guillouf C, Liebermann DA, El-Houseini ME. Induction of p21 (WAF-1/CIP1) during differentiation.Oncogene. 1994;9:3389–3396.PubMedGoogle Scholar
  10. 10.
    Lu CD, Altieri DC, Tanigawa N. Expression of a novel antiapoptosis gene, survivin, correlated with tumor cell apoptosis and p53 accumulation in gastric carcinomas.Cancer Res. 1998;58:1808–1812.PubMedGoogle Scholar
  11. 11.
    Kawasaki H, Altieri DC, Lu CD, Toyoda M, Tenjyo T, Tanigawa N. Inhibition of apoptosis by survivin predicts shorter survival rates in colorectal cancer.Cancer Res. 1998;58:5071–5074.PubMedGoogle Scholar
  12. 12.
    Monzo M, Rosell R, Felip E, et al. A novel anti-apoptosis gene: reexpression of survivin messenger RNA as a prognosis marker in non-small-cell lung cancers.J Clin Oncol. 1999;17:2100–2104.CrossRefPubMedGoogle Scholar
  13. 13.
    Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle checkpoint by survivin.Nature. 1998;396:580–584.CrossRefPubMedGoogle Scholar
  14. 14.
    Tamm I, Wang Y, Sausville E, et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs.Cancer Res. 1998;58:5315–5320.PubMedGoogle Scholar
  15. 15.
    Estrov Z, Thall PF, Talpaz M, et al. Caspase 2 and caspase 3 protein levels as predictors of survival in acute myelogenous leukemia.Blood. 1998;92:3090–3097.PubMedGoogle Scholar

Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Ako Mori
    • 1
  • Hiroshi Wada
    • 1
  • Yasuo Nishimura
    • 1
  • Takahiro Okamoto
    • 1
  • Yoshinobu Takemoto
    • 1
  • Eizo Kakishita
    • 1
  1. 1.Second Department of Internal MedicineHyogo College of MedicineNishinomiyaJapan

Personalised recommendations