International Journal of Hematology

, Volume 75, Issue 1, pp 78–84 | Cite as

Detection of Epstein-Barr Virus and Human T-Cell Lymphotropic Virus Type 1 in Malignant Nodal Lymphoma, Studied in Okinawa, a Subtropical Area in Japan

  • Jun-ichi Miyagi
  • Takayoshi Toda
  • Hiroshi Uezato
  • Kouichi Ohshima
  • Tsuyoshi Miyakuni
  • Nobuyuki Takasu
  • Masato Masuda
Case Report


Formalin-fixed paraffin-embedded lymph node samples were collected from 100 cases of malignant nodal lymphoma documented in Okinawa in the period from 1973 through 1998. According to the new World Health Organization classification, 12 cases were Hodgkin’s lymphoma (HL). Eighty-eight cases of non-Hodgkin’s lymphoma (NHL) included 54 cases of T-cell type and 34 cases of B-cell type. Using polymerase chain reaction (PCR), Epstein-Barr virus (EBV) was detected in 11 cases (91.7%) of HL and in 57 cases (64.8%) of NHL, and human T-cell lymphotropic virus type 1 (HTLV-1) was detected in 23 cases (26.1%) of NHL. Clonal integration of HTLV-1 was detected in 10 (43.5%) of 23 HTLV-1 PCR-positive cases by the inverse PCR technique. The EBV-infected cells were detected by EBER-1 in situ hybridization in 11 (91.7%) of 12 HL cases and in 64 (72.7%) of 88 NHL cases. Irrespective of phenotype and tissue type of the malignant lymphoma, the rate of EBV-positive infection in Okinawa was higher than that in any other districts reported in Japan. This characteristic high rate of EBV-positive infection in Okinawa can be ascribed to various factors, such as racial and geographical differences.

Key words

EBV HTLV-1 Malignant lymphoma Subtropical area Okinawa 


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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Jun-ichi Miyagi
    • 1
  • Takayoshi Toda
    • 2
  • Hiroshi Uezato
    • 3
  • Kouichi Ohshima
    • 4
  • Tsuyoshi Miyakuni
    • 5
  • Nobuyuki Takasu
    • 1
  • Masato Masuda
    • 1
  1. 1.Second Department of Internal Medicine, Faculty of MedicineUniversity of the RyukyusOkinawaJapan
  2. 2.Department of Clinical Laboratory Medicine, Faculty of MedicineUniversity of the RyukyusOkinawaJapan
  3. 3.Department of Dermatology, Faculty of MedicineUniversity of the RyukyusOkinawaJapan
  4. 4.Department of Pathology, School of MedicineFukuoka UniversityFukuokaJapan
  5. 5.Japanese Red Cross Okinawa Blood CenterOkinawaJapan

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