Skip to main content
SpringerLink
Log in

Detection of Epstein-Barr Virus and Human T-Cell Lymphotropic Virus Type 1 in Malignant Nodal Lymphoma, Studied in Okinawa, a Subtropical Area in Japan

  • Case Report
  • Published:
International Journal of Hematology Aims and scope Submit manuscript

Abstract

Formalin-fixed paraffin-embedded lymph node samples were collected from 100 cases of malignant nodal lymphoma documented in Okinawa in the period from 1973 through 1998. According to the new World Health Organization classification, 12 cases were Hodgkin’s lymphoma (HL). Eighty-eight cases of non-Hodgkin’s lymphoma (NHL) included 54 cases of T-cell type and 34 cases of B-cell type. Using polymerase chain reaction (PCR), Epstein-Barr virus (EBV) was detected in 11 cases (91.7%) of HL and in 57 cases (64.8%) of NHL, and human T-cell lymphotropic virus type 1 (HTLV-1) was detected in 23 cases (26.1%) of NHL. Clonal integration of HTLV-1 was detected in 10 (43.5%) of 23 HTLV-1 PCR-positive cases by the inverse PCR technique. The EBV-infected cells were detected by EBER-1 in situ hybridization in 11 (91.7%) of 12 HL cases and in 64 (72.7%) of 88 NHL cases. Irrespective of phenotype and tissue type of the malignant lymphoma, the rate of EBV-positive infection in Okinawa was higher than that in any other districts reported in Japan. This characteristic high rate of EBV-positive infection in Okinawa can be ascribed to various factors, such as racial and geographical differences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hauzen HZ, Schulte-Holthausen H, Klein G, et al. EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx.Nature. 1970;228:1056–1058.

    Article  Google Scholar 

  2. Weiss LM, Movahed LA, Warnke RA, Sklar J. Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin’s disease.N Engl J Med. 1989;320:502–506.

    Article  PubMed  CAS  Google Scholar 

  3. MacMahon EM, Glass JD, Hayward SD, et al. Epstein-Barr virus in AIDS-related primary central nervous system lymphoma.Lancet. 1991;338:969–973.

    Article  PubMed  CAS  Google Scholar 

  4. Sasajima Y, Yamabe H, Kobashi Y, Hirai K, Mori S. High expression of the Epstein-Barr virus latent protein EB nuclear antigen-2 on pyothorax-associated lymphomas.Am J Pathol. 1993;143:1280–1285.

    PubMed  PubMed Central  CAS  Google Scholar 

  5. Harabuchi Y, Yamanaka N, Kataura A, et al. Epstein-Barr virus in nasal T-cell lymphomas in patients with lethal midline granuloma.Lancet. 1990;335:128–130.

    Article  PubMed  CAS  Google Scholar 

  6. Niedobitek G, Herbst H, Young LS, et al. Epstein-Barr virus and carcinomas expression of the viral genome in an undifferentiated gastric carcinoma.Diagn Mol Pathol. 1992;1:103–108.

    Article  PubMed  CAS  Google Scholar 

  7. Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lym- phoma.Proc Natl Acad Sci U S A. 1980;77:7415–7419.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Hinuma Y, Nagata K, Hanaoka M, et al. Adult T-cell leukemia: Antigen in an ATL cell line and detection of antibodies to the antigen in human sera.Proc Natl Acad Sci U S A. 1981;78:6476–6480.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. The T- and B-Cell Malignancy Study Group. Statistical analysis of clinico-pathological, virological and epidemiological data on lymphoid malignancies with special reference to adult T-cell leukemia/lymphoma: a report of the second nationwide study of Japan.Jpn J Clin Oncol. 1985;15:517–535.

    Article  Google Scholar 

  10. Tokunaga M, Imai S, Uemura Y, Tokudome T, Osato T, Sato E. Epstein-Barr virus in adult T-cell leukemia/lymphoma.Am J Pathol. 1993;143:1263–1269.

    PubMed  PubMed Central  CAS  Google Scholar 

  11. Ohshima K, Suzumiya J, Tasiro K, et al. Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non- Hodgkin’s lymphoma of human immunodeficiency virus-negative Japanese.Am J Hematol. 1996;52:21–28.

    Article  PubMed  CAS  Google Scholar 

  12. Harris NL, Jaffe ES, Diebold J, et al. The World Health Organization classification of hematological malignancies report of the clinical advisory committee meeting, Airlie House, Virginia, November 1997.Mod Pathol. 2000;13:193–207.

    Article  PubMed  CAS  Google Scholar 

  13. Uezato H, Hagiwara K, Hosokawa A, et al. Comparative studies of the detection rates of Leishmania parasites from formalin, ethanol- fixed, frozen human skin specimens by polymerase chain reaction and Southern blotting.J Dermatol. 1998;25:623–631.

    Article  PubMed  CAS  Google Scholar 

  14. Imai S, Usui N, Sugiura M, et al. Epstein-Barr virus genomic sequences and specific antibodies in cerebrospinal fluid in children with neurologic complications of acute and reactivated EBV infections.J Med Virol. 1993;40:278–284.

    Article  PubMed  CAS  Google Scholar 

  15. Takemoto S, Matsuoka M, Yamaguchi K, Takatsuki K. A novel diagnostic method of Adult T-cell leukemia: monoclonal integration of Human T-cell lymphotropic virus type I provirus DNA detected by Inverse polymerase chain reaction.Blood. 1994;84:3080–3085.

    PubMed  CAS  Google Scholar 

  16. Lymphoma study group of Japanese pathologists. The World Health Organization classification of malignant lymphomas in Japan: incidence of recently recognized entities.Pathol Int. 2000;50:696–702.

    Article  Google Scholar 

  17. Chang KL, Chen Y-Y, Shibata D, Weiss LM. Description of an in situ hybridization methodology for detection of Epstein-Barr virus RNA in paraffin-embedded tissues, with a survey of normal and neoplastic tissues.Diagn Mol Pathol. 1992;1:246–255.

    Article  CAS  PubMed  Google Scholar 

  18. Li D, Oda K, Mikata A, Yumoto N. Epstein-Barr virus genomes in Hodgkin’s disease and non-Hodgkin’s lymphomas.Pathol Int. 1995;45:735–741.

    Article  PubMed  CAS  Google Scholar 

  19. Zhou XG, Hamilton-Dutoit SJ, Yan QH, Pallesen G. The association between Epstein-Barr virus and Chinese Hodgkin’s disease.Int J Cancer. 1993;55:359–363.

    Article  PubMed  CAS  Google Scholar 

  20. Chan JKC, Yip TTC, Tsang WYW, Lau W, Wong CSC, Ma VWS. Detection of Epstein-Barr virus in Hodgkin’s disease occurring in an oriental population.Hum Pathol. 1995;26:314–318.

    Article  PubMed  CAS  Google Scholar 

  21. Tomita Y, Ohsawa M, Kanno H, et al. Epstein-Barr virus in Hodgkin’s disease patients in Japan.Cancer. 1996;77:186–192.

    Article  PubMed  CAS  Google Scholar 

  22. Kusuda M, Toriyama K, Kamidigo NO, Itakura H. A comparison of epidemiologic, histologic, and virologic studies on Hodgkin’s disease in western Kenya and Nagasaki, Japan.Am J Trop Med Hyg. 1998;59:801–807.

    Article  PubMed  CAS  Google Scholar 

  23. Ambinder RF, Browning PJ, Lorenzana I, et al. Epstein-Barr virus and childhood Hodgkin’s disease in Honduras and the United States.Blood. 1993;81:462–467.

    PubMed  CAS  Google Scholar 

  24. Leoncini L, Spina D, Nyong’o A, et al. Neoplastic cells of Hodg- kin’s disease show differences in EBV expression between Kenya and Italy.Int J Cancer. 1996;65:781–784.

    Article  PubMed  CAS  Google Scholar 

  25. Chang KL, Albujar PF, Chen Y-Y, Johnson RM, Weiss LM. High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin’s disease occurring in Peru.Blood. 1993;81:496–501.

    PubMed  CAS  Google Scholar 

  26. Korbjuhn P, Anagnostopoulos I, Hummel M, et al. Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas.Blood. 1993;82:217–223.

    PubMed  CAS  Google Scholar 

  27. Hirose Y, Masaki Y, Shimizu S, et al. Association of Epstein-Barr virus with human immunodeficiency virus-negative T-cell lym- phomas in Japan, in situ hybridization, polymerase chain reaction and immunohistochemical studies.Leukemia. 1996;10:1673–1675.

    PubMed  CAS  Google Scholar 

  28. Hummel M, Anagnostopoulos I, Korbjuhn P, Stein H. Epstein-Barr virus in B-cell non-Hodgkin’s lymphomas: unexpected infection patterns and different infection incidence in low-and high-grade types.J Pathol. 1995;175:263–271.

    Article  PubMed  CAS  Google Scholar 

  29. Herbst H, Dallenbach F, Hummel M, et al. Epstein-Barr virus DNA and latent gene products in Ki-1 (CD30)-positive anaplastic large cell lymphomas.Blood. 1991;78:2666–2673.

    PubMed  CAS  Google Scholar 

  30. Hirose Y, Masaki Y, Sasaki K, et al. Determination of Epstein-Barr virus association with B-cell lymphomas in Japan: study of 72 cases-in situ hybridization, polymerase chain reaction, immuno-histochemical studies.Int J Hematol. 1998;67:165–174.

    Article  PubMed  CAS  Google Scholar 

  31. Mishima K, Horiuchi K, Kojya S, Takahashi H, Ohsawa M, Aozasa K. Epstein-Barr virus in patients with polymorphic reticulosis (lethal midline granuloma) from China and Japan.Cancer. 1994;73:3041–3046.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Second Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Jun-ichi Miyagi, Nobuyuki Takasu & Masato Masuda

  2. Department of Clinical Laboratory Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Takayoshi Toda

  3. Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Hiroshi Uezato

  4. Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan

    Kouichi Ohshima

  5. Japanese Red Cross Okinawa Blood Center, Okinawa, Japan

    Tsuyoshi Miyakuni

Authors
  1. Jun-ichi Miyagi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takayoshi Toda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroshi Uezato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kouichi Ohshima
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tsuyoshi Miyakuni
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nobuyuki Takasu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Masato Masuda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takayoshi Toda.

About this article

Cite this article

Miyagi, Ji., Toda, T., Uezato, H. et al. Detection of Epstein-Barr Virus and Human T-Cell Lymphotropic Virus Type 1 in Malignant Nodal Lymphoma, Studied in Okinawa, a Subtropical Area in Japan. Int J Hematol 75, 78–84 (2002). https://doi.org/10.1007/BF02981984

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981984

Key words

Search

Navigation