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International Journal of Hematology

, Volume 75, Issue 1, pp 72–77 | Cite as

Establishment of a Monosomy 7 Leukemia Cell Line, MONO-7, With aras Gene Mutation

  • Hiroyuki Fujisaki
  • Kenji Takai
  • Akihisa Sawada
  • Sadao Tokimasa
  • Yoshiko Matsuda
  • Hideaki Ohta
  • Yuko Osugi
  • Ji Yoo Kim
  • Gaku Hosoi
  • Masahiro Sako
  • Junichi Hara
Case Report

Abstract

A monosomy 7 leukemia cell line, designated MONO-7, was established from the peripheral blood of a patient with monosomy 7 acute myelocytic leukemia (French-American-British classification M0). The cells were cultured continuously for more than 24 months in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum. The cell line exhibits an unclassified appearance. Cytochemically, α-naphthol-acetate esterase and myeloperoxidase are negative. Immunophenotypi-cally, the cell line expresses CD33, CD13, CD56, CD34, CD38, HLA-DR, and CD45, but lacks T and B cell-associated antigens. Karyotypic analysis of the cell line showed only 45,XY,-7. Analysis of the N-ras gene mutation demonstrated identical mutations in fresh leukemic cells and the MONO-7 cell line. Clonal rearrangements of the immunoglobulin heavy-chain gene, T-cell receptor β-chain gene, or T-cell receptor γ-chain gene were not found in DNA extracted from MONO-7 cells. The growth of MONO-7 cells in vitro was stimulated by recombinant human granulocyte-macrophage colony-stimulating factor or interleukin 3. To our knowledge, this is the first report of the establishment of a cell line with the karyotype 45,XY,-7 with-out any other abnormality and with a ras gene mutation.

Key words

Monosomy 7 Leukemia Cell line ras gene mutation 

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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Hiroyuki Fujisaki
    • 1
  • Kenji Takai
    • 1
  • Akihisa Sawada
    • 1
  • Sadao Tokimasa
    • 1
  • Yoshiko Matsuda
    • 1
  • Hideaki Ohta
    • 1
  • Yuko Osugi
    • 1
  • Ji Yoo Kim
    • 1
  • Gaku Hosoi
    • 2
  • Masahiro Sako
    • 2
  • Junichi Hara
    • 1
  1. 1.Department of Developmental Medicine (Pediatrics), D-5Osaka University Graduate School of MedicineOsakaJapan
  2. 2.Department of PediatricsOsaka City General HospitalOsakaJapan

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