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International Journal of Hematology

, Volume 75, Issue 1, pp 55–62 | Cite as

The Generation of Immunocompetent Dendritic Cells From CD34+ Acute Myeloid or Lymphoid Leukemia Cells

  • Takahide Tsuchiya
  • Masao Hagihara
  • Yasuhito Shimakura
  • Yoko Ueda
  • Balgansuren Gansuvd
  • Batmunkh Munkhbat
  • Hiroyasu Inoue
  • Kei Tazume
  • Shunichi Kato
  • Tomomitsu Hotta
Case Report

Abstract

The ability of CD34+ leukemic cells to differentiate to dendritic cells (DCs) was investigated in 18 acute myeloid leukemia (AML) and 4 lymphoid leukemia (ALL) patients. The generation of DCs was determined by the expression of DC-associated CD1a or CD83 (more than 30%) with costimulatory molecules, by CD80 antigens (>20%), and by the exhibition of allostim-ulatory activity. In the AML patients, allostimulatory mature DCs were generated from 3 of 9 M0 or M1, 2 of 5 M2, 2 of 4 M4 or M5, and 3 of 4 ALL (L2) cases. In total, DCs were more efficiently induced from cases expressing over 75% of CD34+ among whole bone marrow mononuclear cells (8 of 12), compared with those under 75% (2 of 10; P < .05). B-cell (CD19), natural killer (NK)—cell (CD56), or T-cell (CD7) lineage markers, which were aberrantly expressed on the blasts, were rarely found on leukemic DCs at the end of the culture period, and myeloid (CD13, CD33), not lymphoid (CD10), markers were shown on ALL-derived DCs. In Philadelphia chromosome—positive ALL or AML patients with t (8;21), DCs were confirmed to be of leukemic origin by fluorescence in situ hybridization analysis.

Key words

Dendritic cells Leukemia CD34 

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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Takahide Tsuchiya
    • 1
  • Masao Hagihara
    • 1
  • Yasuhito Shimakura
    • 1
  • Yoko Ueda
    • 1
  • Balgansuren Gansuvd
    • 1
  • Batmunkh Munkhbat
    • 2
  • Hiroyasu Inoue
    • 3
  • Kei Tazume
    • 1
  • Shunichi Kato
    • 2
    • 3
  • Tomomitsu Hotta
    • 1
  1. 1.Department of Hematology and RheumatologyTokai University School of MedicineKanagawaJapan
  2. 2.Research Center for Genetic Engineering and Cell TransplantationTokai University School of MedicineKanagawaJapan
  3. 3.Department of PediatricsTokai University School of MedicineKanagawaJapan

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