International Journal of Hematology

, Volume 73, Issue 3, pp 327–334 | Cite as

Platelet-Derived Growth Factor Enhances Granulopoiesis Via Bone Marrow Stromal Cells

  • Mo Yang
  • Karen Li
  • Audrey Carmen Lam
  • Patrick Man Pan Yuen
  • Tai Fai Fok
  • Colin N. Chesterman
  • Beng H. Chong
Rapid Communication


Platelet-derived growth factor (PDGF), a growth factor for connective tissue cells, stimulates erythropoiesis and megakaryocytopoiesis in vitro but the effect of PDGF on granulocyte proliferation remains unknown.The effect of the recombinant human PDGF-BB isoform on granulopoiesis was investigated in this study. The results show that PDGF significantly stimulated murine colony-forming unit-granulocyte-monocyte (CFU-GM) proliferation in a dose-dependent manner (1 to 100 ng/mL) using murine bone marrow cells (n = 4). Maximum stimulation was obtained with 50 ng/mL of PDGF (P < .01). The effect of PDGF on murine CFU-GM proliferation was compared with that of interleukin (IL)-3, IL-6, granulocyte-monocyte colony-stimulating factor (GM-CSF),and acidic fibroblast growth factor (aFGF) at their optimal doses.The stimulating activity of PDGF was higher than that of aFGF but lower than that of IL-3, IL-6, or GM-CSF. There is no synergistic effect between PDGF and IL-3 or IL-6, but a significant enhancing effect was observed in IL-3 plus IL-6. PDGF also stimulated the growth of CFU-GM with CFU-megakaryocyte in the presence of bone marrow stromal cells. We also found that PDGF had similar a effect on human CFU-GM proliferation using bone marrow mononuclear cells (MNC). However, the increase in PDGF-stimulated CFU-GM proliferation was inhibited by anti-GM-CSF, anti-IL-3, and anti-IL-6 antibodies (n = 4), suggesting that endogenously produced GM-CSF, IL-3, and IL-6 may play a role in the PDGF-induced CFU-GM proliferation. Furthermore, PDGF (1 to 100 ng/mL) did not show any effect on CFU-GM proliferation when replacing bone marrow MNC with immunomagnetic selection-enriched CD34+ cells from human cord blood (n = 5; purity, 91% +- 6.5%).This study indicates that PDGF may indirectly enhance CFU-GM proliferation by inducing the bone marrow stromal cells to produce GM-CSF, IL-3, or IL-6.

Key words

PDGF CFU-GM CFU-MK Stromal cells 


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Copyright information

© The Japanese Society of Hematology 2001

Authors and Affiliations

  • Mo Yang
    • 1
  • Karen Li
    • 1
  • Audrey Carmen Lam
    • 1
  • Patrick Man Pan Yuen
    • 1
  • Tai Fai Fok
    • 1
  • Colin N. Chesterman
    • 2
  • Beng H. Chong
    • 2
  1. 1.Department of Paediatrics, Prince of Wales HospitalThe Chinese University of Hong KongHong KongAustralia
  2. 2.Centre for Thrombosis and Vascular Research, School of PathologyThe University of New South WalesSydneyAustralia

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