International Journal of Hematology

, Volume 73, Issue 2, pp 236–244 | Cite as

A Hodgkin’s Disease Cell Line, KM-H2, Shows Biphenotypic Features of Dendritic Cells and B Cells

  • Kazutaka Uehira
  • Ryuichi Amakawa
  • Tomoki Ito
  • Tomoko Uehira
  • Yoshio Ozaki
  • Toshiki Shimizu
  • Masahiro Fujimoto
  • Muneo Inaba
  • Shirou Fukuhara
Case Report

Abstract

The origin of Reed-Sternberg (RS) cells, the neoplastic cells of Hodgkin’s disease, has long remained controversial. Dendritic cells (DCs) are highly specialized antigen-presenting cells that have the unique capacity to prime naive T cells, and they may be progenitors of RS cells in a population of Hodgkin’s disease cells. In this study, the KM-H2 cell line, previously established from a patient with Hodgkin’s disease of mixed cellularity, was reevaluated for its cellular derivation, particularly in terms of DCs. KM-H2 cells were demonstrated to carry the newly proposed DC-associated molecules fascin, CD83, and DEC-205, as well as costimulatory molecules such as CD40, CD80, and CD86. In addition, KM-H2 cells were shown to be able to potently stimulate peripheral blood T cells and to have the strong endocytotic activity of fluorescein isothiocyanate—dextran. On the other hand, KM-H2 cells were shown to have variable-diversity-joining recombination of the immunoglobulin H gene, although they did not express any subclasses of immunoglobulin and they were negative for CD79a and CD79b. In addition, KM-H2 cells produced the messenger RNA of thePax-5 gene.These findings lead to a hypothesis that KM-H2 cells originated from the cells that had differentiated through the possible common DC—B-cell progenitors along the newly proposed pathway.

Key words

KM-H2 cell line Hodgkin’s disease Reed-Sternberg cells Dendritic cells Fascin 

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Copyright information

© The Japanese Society of Hematology 2001

Authors and Affiliations

  • Kazutaka Uehira
    • 1
  • Ryuichi Amakawa
    • 1
  • Tomoki Ito
    • 1
  • Tomoko Uehira
    • 1
  • Yoshio Ozaki
    • 1
  • Toshiki Shimizu
    • 1
  • Masahiro Fujimoto
    • 1
  • Muneo Inaba
    • 2
  • Shirou Fukuhara
    • 1
  1. 1.First Department of Internal MedicineKansai Medical UniversityOsakaJapan
  2. 2.First Department of PathologyKansai Medical UniversityOsakaJapan

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