Advertisement

International Journal of Hematology

, Volume 73, Issue 2, pp 199–205 | Cite as

Effect of Granulocyte Colony-Stimulating Factor on Chemotherapeutic Activity of Cytosine Arabinoside in Acute Leukemic Cell Lines

  • Kwang Eun Cha
  • Soo Young Yoon
  • Kap No Lee
Review Article
  • 37 Downloads

Abstract

Recent studies have shown the presence of receptors for granulocyte colony-stimulating factor (G-CSF) on lymphoid leukemic cells. To determine the effect of G-CSF on chemotherapeutic activity of cytosine arabinoside (Ara-C) on lymphoid as well as myeloid leukemic cells, we evaluated cell counts, apoptosis, and growth inhibition in HL-60, KG-1, Molt-4, Jijoye, and CCRF-CEM cell lines after incubation with Ara-C (0.1 and 1 μmol/L) and/or 5 ng/mL G-CSF. G-CSF potentiated the effect of Ara-C on 2 of 3 lymphoid leukemic cell lines (Molt-4 and Jijoye), whereas it decreased the apoptosis and the effect of Ara-C on myeloid cell lines (HL-60 and KG-1).

Key words

Granulocyte colony-stimulating factor Cytosine arabinoside Leukemic cell lines Apoptosis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Demetri GD, Griffin JD. Granulocyte colony-stimulating factor and its receptor.Blood. 1991;78:2791–2808.PubMedGoogle Scholar
  2. 2.
    Bradbury D, Zhu YM, Russell N. Regulation of Bcl-2 expression and apoptosis in acute myeloblastic leukaemia cells by granulocyte-macrophage colony-stimulating factor.Leukemia. 1994;8:786–791.PubMedGoogle Scholar
  3. 3.
    Butturini A, Santucci MA, Gale RP, Perocco P, Tura S. GM-CSF incubation prior to treatment with cytarabine or doxorubicin enhances drug activity against AML cells in vitro: a model for leukemia chemotherapy.Leuk Res. 1990;14:743–749.CrossRefPubMedGoogle Scholar
  4. 4.
    Baer MR, Bernstein SH, Brunetto VL, et al. Biological effects of recombinant human granulocyte colony-stimulating factor in patients with untreated acute myeloid leukemia.Blood. 1996;87:1484–1494.PubMedGoogle Scholar
  5. 5.
    te Boekhorst PA, Lowenberg B, Vlastuin M, Sonneveld P. Enhanced chemosensitivity of clonogenic blasts from patients with acute myeloid leukemia by G-CSF, IL-3 or GM-CSF stimulation.Leukemia. 1993;7:1191–1198.Google Scholar
  6. 6.
    Higashigawa M, Komada Y, Washio N, et al. Recombinant human granulocyte colony-stimulating factor enhanced cytotoxicity of Ara-C in Ara-C-resistant leukemic cells from a patient with biphenotypic leukemia in cell kinetic quiescence.Leuk Res. 1992;16:1049–1054.CrossRefPubMedGoogle Scholar
  7. 7.
    Bai A, Kojima H, Hori M, et al. Priming with G-CSF effectively enhances low-dose Ara-C-induced in vivo apoptosis in myeloid leukemia cells.Exp Hematol. 1999;27:259–265.CrossRefPubMedGoogle Scholar
  8. 8.
    Tosi P, Visani G, Ottaviani E, et al. Fludarabine+ Ara-C+ G-CSF: cytotoxic effect and induction of apoptosis on fresh acute myeloid leukemia cells.Leukemia. 1994;8:2076–2082.PubMedGoogle Scholar
  9. 9.
    Estey E, Thall P, Andreeff M, et al. Use of granulocyte colony-stimulating factor before, during, and after fludarabine plus cytarabine induction therapy of newly diagnosed acute myelogenous leukemia or myelodysplastic syndromes: comparison with fludarabine plus cytarabine without granulocyte colony-stimulating factor.J Clin Oncol. 1994;12:671–678.CrossRefPubMedGoogle Scholar
  10. 10.
    Lotem J, Sachs L. Hematopoietic cytokines inhibit apoptosis induced by transforming growth factor beta 1 and cancer chemotherapy compounds in myeloid leukemic cells.Blood. 1992;80:1750–1757.PubMedGoogle Scholar
  11. 11.
    Buchner T, Hiddemann W, Koenigsmann M, et al. Recombinant human granulocyte-macrophage colony-stimulating factor after chemotherapy in patients with acute myeloid leukemia at higher age or after relapse.Blood. 1991;78:1190–1197.PubMedGoogle Scholar
  12. 12.
    Stone RM, Berg DT, George SL, et al. Granulocyte-macrophage colony-stimulating factor after initial chemotherapy for elderly patients with primary acute myelogenous leukemia: Cancer and Leukemia Group B.N Engl J Med. 1995;332:1671–1677.CrossRefPubMedGoogle Scholar
  13. 13.
    Koenigsmann M, Hiddemann W, Buchner T. Reversible leukaemic regrowth under GM-CSF treatment after chemotherapy for AML.Leuk Res. 1991;15:37–41.CrossRefPubMedGoogle Scholar
  14. 14.
    Shinjo K, Takeshita A, Ohnishi K, Ohno R. Granulocyte colony-stimulating factor receptor at various differentiation stages of normal and leukemic hematopoietic cells.Leuk Lymphoma. 1997;25:37–46.CrossRefPubMedGoogle Scholar
  15. 15.
    Drach D, Estrov Z, Zhao S, et al. Granulocyte-colony stimulating factor, granulocyte-macrophage colony stimulating factor, PIXY-321, stem cell factor, interleukin-3, and interleukin-7: receptor binding and effects on clonogenic proliferation in acute lymphoblastic leukemia.Leuk Lymphoma. 1994;16:79–88.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Kita K, Nishii K, Ohishi K, et al. Frequent gene expression of granulocyte colony-stimulating factor (G-CSF) receptor in CD7+ surface CD3 acute lymphoblastic leukaemia.Leukemia. 1993;7:1184–1190.Google Scholar
  17. 17.
    Inukai T, Sugita K, Iijima K, et al. Expression of granulocyte colony-stimulating factor receptor on CD10-positive human B-cell precursors.Br J Haematol. 1995;89:623–626.CrossRefGoogle Scholar
  18. 18.
    Corcione A, Tasso P, Pistoia V. The granulocyte colony-stimulating factor (G-CSF)/G-CSF receptor (G-CSFR) system in B-cell chronic lymphocytic leukemia.Leuk Lymphoma. 1997;27:239–246.CrossRefGoogle Scholar
  19. 19.
    Inukai T, Sugita K, Iijima K, et al. Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF.Leukemia. 1998;12:382–389.CrossRefGoogle Scholar
  20. 20.
    Schmid I, Uittenbogaart CH, Keld B, Giorgi JV. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry.J Immunol Methods. 1994;170:145–157.CrossRefPubMedGoogle Scholar
  21. 21.
    Schmid I, Uittenbogaart CH, Giorgi JV. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry.Cytometry. 1994;15:12–20.CrossRefPubMedGoogle Scholar
  22. 22.
    Philpott NJ, Turner AJ, Scopes J, et al. The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques.Blood. 1996;87:2244–2251.PubMedGoogle Scholar
  23. 23.
    Vellenga E, Young DC, Wagner K, et al. The effects of GM-CSF and G-CSF in promoting growth of clonogenic cells in acute myeloblastic leukemia.Blood. 1987;69:1771–1776.PubMedGoogle Scholar
  24. 24.
    Nara N, Murohashi I, Suzuki T, et al. Effects of recombinant human granulocyte colony-stimulating factor (G-CSF) on blast progenitors from acute myeloblastic leukaemia patients.Br J Cancer. 1987;56:49–51.CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Young DC, Demetri GD, Ernst TJ, Cannistra SA, Griffin JD. In vitro expression of colony-stimulating factor genes by human acute myeloblastic leukemia cells.Exp Hematol. 1988;16:378–382.PubMedGoogle Scholar
  26. 26.
    Souza LM, Boone TC, Gabrilove J, et al. Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.Science. 1986;232:61–65.CrossRefPubMedGoogle Scholar
  27. 27.
    Nicola NA, Begley CG, Metcalf D. Identification of the human analogue of a regulator that induces differentiation in murine leukaemic cells.Nature. 1985;314:625–628.CrossRefPubMedGoogle Scholar
  28. 28.
    Fujiwara H, Arima N, Matsushita K, et al. Granulocyte-colony stimulating factor induces differentiation and apoptosis of CD2, CD7 positive hybrid leukemia cells in vivo and ex vivo.Leuk Res. 1997;21:735–741.CrossRefPubMedGoogle Scholar
  29. 29.
    Elias L, Van Epps DE. Early effects of G- and GM-CSF upon HL-60 proliferation and differentiation.Leukemia. 1988;2:763–765.PubMedGoogle Scholar
  30. 30.
    Begley CG, Metcalf D, Nicola NA. Purified colony stimulating factors (G-CSF and GM-CSF) induce differentiation in human HL60 leukemic cells with suppression of clonogenicity.Int J Cancer. 1987;39:99–105.CrossRefPubMedGoogle Scholar
  31. 31.
    Ohyashiki JH, Ohyashiki K, Miyauchi J, et al. Immunogenotypes and clonal culture analysis in B-precursor acute lymphoblastic leukemia.Leukemia. 1992;6:240–245.PubMedGoogle Scholar
  32. 32.
    Till KJ, Burthem J, Lopez A, Cawley JC. Granulocyte-macrophage colony-stimulating factor receptor: stage-specific expression and function on late B cells.Blood. 1996;88:479–486.PubMedGoogle Scholar

Copyright information

© The Japanese Society of Hematology 2001

Authors and Affiliations

  • Kwang Eun Cha
    • 1
  • Soo Young Yoon
    • 2
  • Kap No Lee
    • 2
  1. 1.Department of PediatricsCollege of Medicine, Pochon Cha UniversityPochon
  2. 2.Department of Clinical PathologyKorea University Medical SchoolSeoulKorea

Personalised recommendations