Abstract
Banana plants expressing mosaic symptoms, from the Jordan Valley in Israel, were shown to be infected by a satellite-RNA-containing strain of cucumber mosaic virus (CMV). Double-stranded RNA isolated from field-infected banana, without passage through another host, was used as a template for synthesis of cDNA. The cDNAs corresponding to the coat protein (CP) gene and to the satellite RNA were cloned after polymerase chain reaction amplification. The nucleotide sequences of the CP and the satellite cDNAs were determined. The CP gene and its 3′ flanking sequence had 98% similarity to the CMV Fny nucleotide sequence and the two strains differed in only one amino acid of the CP. The associated satellite had a sequence similarity ranging from 95% to 85.6% with other CMV satellites. Analysis of banana suckers differing in symptoms’ severity indicated a correlation between the presence of satellite and attenuation of symptoms.
Similar content being viewed by others
References
Caruana, M.L. (1992) Principal virus diseases of banana.in: Breeding Banana and Plantain for Resistance to Diseases and Pests.Proc. Int. Symp. on Genetic Improvement of Bananas for Resistance to Diseases and Pests (Montpellier, France), pp. 99–103.
Crescenzi, A., Barbarossa, L., Gallitelli, D. and Martelli, G.P. (1993) Cucumber mosaic cucumovirus populations in Italy under natural epidemic conditions and after a satellite-mediated protection test.Plant Dis. 77:28–33.
Devereaux, J., Heaberli, P. and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX.Nucleic Acids Res. 12:387–395.
Dodds, J.A. (1993) dsRNA in diagnosis.in: Matthews, R.E.F. [Ed.] Diagnosis of Plant Virus Diseases. pp. 273–294. CRC Press Inc., Boca Raton, FL, USA.
Dow, R.L. (1985) Virus confirmation.Bermuda Dep. Agric. Fish., Mon. Bull. 56(3):20–21.
Fagoaga, C., Pina, J.A. and Duran-Vila, N. (1994) Occurrence of small RNAs in severely diseased vegetable crops.Plant Dis. 78:749–753.
Francki, R.I.B. (1985) Plant virus satellites.Annu. Rev. Microbiol. 39:151–174.
Garcia-Arenal, F., Zaitlin, M. and Palukaitis, P. (1987) Nucleotide sequence analysis of six satellite RNAs of cucumber mosaic virus: Primary sequence and secondary structure alterations do not correlate with differences in pathogenicity.Virology 158:339–347.
Gonsalves, D., Provvidenti, R. and Edwards, M.C. (1982) Tomato white leaf: The relation of an apparent satellite RNA and cucumber mosaic virus.Phytopathology 72:1533–1538.
Grieco, F., Cillo, F., Barbarossa, L. and Gallitelli, D. (1992) Nucleotide sequence of a cucumber mosaic virus satellite RNA associated with a tomato top stunting.Nucleic Acids Res. 20:6733.
Igloi, G.L. (1983) A silver stain for the detection of nanogram amounts of tRNA following twodimensional electrophoresis.Anal. Biochem. 134:184–188.
Jorda, C.A., Alfaro, M.A., Aranda, E. and Garcia-Arenal, F. (1992) Epidemic of cucumber mosaic virus plus satellite RNA in tomatoes in Eastern Spain.Plant Dis. 76:363–366.
Kaper, J.M. (1993) Viral satellites, molecular parasites for plant protection.in: Lumsden, R.D. and Vaughn, J. L. [Eds.] Pest Management: Biologically Based Technologies, pp. 134–143. American Chemical Society, Washington, DC.
Kaper, J.M. and Woaterworth, H.E. (1977) Cucumber mosaic virus-associated RNA 5: causal agent for tomato necrosis.Science 196:429–431.
Kearney, C.M., Zitter, T.A. and Gonsalves, D. (1990) A field survey for serogroups and satellite RNA of cucumber mosaic virus.Phytopathology 80:1238–1243.
16. Lot, H. and Kaper, J.M. (1976) Further studies on the RNA component distribution among the nucleoproteins of cucumber mosaic virus.Virology 74:223–226.
Masuta, C. and Takanami, Y. (1989) Determination of sequence and structural requirements for pathogenicity of a cucumber mosaic virus satellite RNA (Y-satRNA).Plant Cell 1:1165–1173.
Nitzany, F.E. (1960) Natural hosts of cucumber mosaic virus in Israel.Plant Dis. Rep. 44:144–146.
Owen, J., Shintaku, M., Aeschleman, P., Ben Tahar, S. and Palukaitis, P. (1990) Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 3.J. Gen. Virol. 71:2243–2249.
Palukaitis, P., Roossinck, M.J., Dietzgen, R.G. and Francki, R.I.B. (1992) Cucumber mosaic virus.Adv. Virus Res. 41:281–348.
Roossinck, M.J., Sleat, D. and Palukaitis, P. (1992) Satellite RNAs of plant viruses: structure and biological effects.Microbiol. Rev. 56:265–279.
Rosner, A., Bar-Joseph,M., Moscovitz, M. and Mevarech, M. (1983) Diagnosis of specific viral RNA sequences in plant extracts by hybridization with a polynucleotide kinase mediated,32Plabeled, double-stranded RNA probe.Phytopathology 73:699–702.
Sambrook, J., Fritsch, E. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
Takanami, Y. (1981) A striking change in symptoms on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA.Virology 109:120–126.
Wexler, A., Mawassi, M., Lachman, O., Amit, B., Wortzel, A. and Bar-Joseph, M. (1991) A procedure to amplify cDNA from dsRNA templates using the polymerase chain reaction.Methods Mol. Cell Biol. 2:273–279.
Yot-Dauthy, D. and Bové, J.M. (1966) Mosaiques du bananier: identification et purification de diverses souches du virus.Fruits d’Outre Mer 21:449–466.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Gafny, R., Wexler, A., Mawassi, M. et al. Natural infection of banana by a satellite-containing strain of cucumber mosaic virus: nucleotide sequence of the coat protein gene and the satellite RNA. Phytoparasitica 24, 49–56 (1996). https://doi.org/10.1007/BF02981453
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02981453