Skip to main content
Log in

Lipid metabolism in tomato and bean as a sensitive monitor for biocontrol of wilt diseases

  • Phytopathology
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

The lipids metabolism of tomato and bean plants during biological control of wilt pathogens (Fusarium oxysporum f.sp.lycopersici andF. oxysporum f.sp.phaseoli, respectively) byBacillus subtilis was investigated. The interaction of wilt pathogens with both tomato and bean caused an imbalance and drastic reduction in total lipids, triacylglycerol, sterol and all phospholipd fractions except phosphatidic acid. The application of a formulated biocontrol agent,B. subtilis, eliminated the detrimental effect of both wilt pathogens and consequently prevented catabolism of lipid fractions in both tomato and bean. Moreover, the changes in the lipid fractions as a sensitive monitor for biocontrol of wilt diseases suggest a positive correlation between the application ofB. subtilis and improvement in the host metabolism towards anabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Abd-Allah, E.F. (2005) Effect of aBacillus subtilis isolate on southern blight (Sclerotium rolfsii) and lipid composition of peanut seeds.Phytoparasitica 33:460–466.

    Article  Google Scholar 

  2. Abd-Allah, F.F., El-Didamony, G. and Sarhan, M.M. (1997) Biological control ofFusarium oxysporum in bean byBacillus subtilis.Egypt. J. Phytopathol. 25:71–83.

    Google Scholar 

  3. Abd-Allah, E.F. and Ezzat, S.M. (2004) Role of lipid metabolism through bioremediation of fusaric acid in germinating peanut seedlings.Phytoparasitica 32:38–42.

    Article  CAS  Google Scholar 

  4. Amenta, J.S. (1964) A rapid method for quantification of lipids separated by thin layer chromatography.J. Lipid Res. 5:270–272.

    PubMed  CAS  Google Scholar 

  5. Cachorro, P., Ortiz, A. and Cerda, A. (1993) Effect of saline stress and calcium on lipid composition in bean roots.Phytochemistry 32:1131–1136.

    Article  CAS  Google Scholar 

  6. Daniel, W.W. (1987) Biostatistics: A Foundation for Analysis in the Health Sciences. 4th ed. John Wiley and Sons, New York, NY. pp. 292–293.

    Google Scholar 

  7. El-Banoby, F.E. and Rudolf, K. (1979) A polysaccharide from liquid cultures ofPseudomonas phaseolicola which specifically induces water-soaking in bean leaves (Phaseolus vulgaris L.).Phytopathol. Z. 95:38–50.

    Article  CAS  Google Scholar 

  8. El-Hoseiny, M.M. and Rabie, M.R. (1979) Effect of bacterization of maize (Zea mays) withAzotobacter chroococcum on their growth.Egypt. J. Microbiol. 14:45–57.

    CAS  Google Scholar 

  9. Fölch, J., Lees, M. and Sloane-Stanley, G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissues.J. Biol. Chem. 226:497–509.

    PubMed  Google Scholar 

  10. Hegazy, H.S. and Lösel, D.M. (1992) Effect of bacterial infection on plant lipid metabolism.Proc. Tenth International Symp. on Plant Lipids (Jerba, Tunisia), pp. 331–334.

  11. Hitchcock, C. (1975) Structure and distribution of plant acyl lipids.in: Galliard, T. and Mercer, E.I. [Eds.] Recent Advances in the Chemistry and Biochemistry of Plant Lipids. Academic Press, New York, NY. pp. 1–19.

    Google Scholar 

  12. Hitchcock, C. and Nichols, B.W. [Eds.] (1971) Plant Lipid Biochemistry. Academic Press, New York, NY. p. 68.

    Google Scholar 

  13. Jiang, H.F. and Duan, N.X. (1997) [Further identification and comprehensive evaluation of pre-selected groundnut germplasm.]Oil Crops of China 19:57–59 (Chinese; English abstract inCAB Abstracts 1996–1998/07).

    Google Scholar 

  14. Kates, M. and Marshall, M.O. (1975) Biosynthesis of phosphoglycerides in plants.in: Galliard, T. and Mercer, E.I. [Eds.] Recent Advances in the Chemistry and Biochemistry of Plant Lipids. Academic Press, New York, NY. pp. 115–159.

    Google Scholar 

  15. Mahadevan, A. [Ed.] (1984) Growth Regulators and Diseased Plants. Mohan Primlani for Oxford & IBH Publishing Co., New Delhi, India.

    Google Scholar 

  16. Marsh, J.B. and Weinstein, D.B. (1966) Simple charring method for determination of lipids.J. Lipid Res. 7:574–576.

    PubMed  CAS  Google Scholar 

  17. Mazliak, P., Douady, D., Demandre, C. and Kader, J.C. (1975) Exchange processes between organelles involved in membrane lipid biosynthesis.in: Galliard, T. and Mercer, E.I. [Eds.] Recent Advances in the Chemistry and Biochemistry of Plant Lipids. Academic Press, New York, NY. pp. 301–318.

    Google Scholar 

  18. Miraazimova, K.H.T., Gusakova, S.D. and Glushenkova, A.I. (1997) Post-infectional changes on the phospholipids of the leaves of cotton plant variety resistant toVerticillium dahliae.Chem. Nat. Compd. (Engl. Transl. Khim. Prir. Soedin.) 33:156–161.

    Google Scholar 

  19. Navari-Izzo, F., Quartacci, M.F. and Izzo, R. (1993) Lipid composition of plasma membranes isolated from sunflower seedlings grown under water stress.Physiol. Plant. 87:508–514.

    Article  CAS  Google Scholar 

  20. Radwan, S.S. and Mangold, H.K. (1976) The lipids of plant tissue cultures.Adv. Lipid Res. 12:171–211.

    Google Scholar 

  21. Rouser, G., Fleischer, S. and Yamamoto, A. (1970) Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids and phosphorus analysis of spots.Lipids 5:494–496.

    Article  PubMed  CAS  Google Scholar 

  22. Talboys, P.W. (1968) Water deficit in vascular diseases.in: Kozlowski, T.T. [Ed.] Water Deficits and Plant Growth. Academic Press, New York, NY. pp. 255–311.

    Google Scholar 

  23. Whitney, P.J. (1976) Disturbance of host metabolism.in: Whitney, P.J. [Ed.] Microbial Plant Pathology. Academic Press, New York, NY. pp. 109–132.

    Google Scholar 

  24. Yamada, T. (1987) Lipid peroxidation during the development of pine wilt disease.Ann. Phytopathol. Soc. Jpn. 53:523–530.

    CAS  Google Scholar 

  25. Yunusova, S.G., Gusakova, S.D., Miraazimova, K.T., Glushenkova, A.I., Usmanov, S.A. and Ikramov, Y. (1992) Effect of a wilt infection on the neutral lipids of cotton leaves.Chem. Nat. Compd. (Engl. Transl. Khim. Prir. Soedin.) 28:414–420.

    Google Scholar 

  26. Zeller, W., Rudolph, K. and Hoppe, H.-H. (1976) Effect ofPseudomonas phaseolicola toxin on the composition of lipids in leaves of swiss chard (Beta vulgaris L.).Phytopathol. Z. 86:205–214.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to E. F. Abd-Allah.

Additional information

http://www.phytoparasitica.org posting Sept. 20, 2006.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abd-Allah, E.F., Hashem, A. & Ezzat, S.M. Lipid metabolism in tomato and bean as a sensitive monitor for biocontrol of wilt diseases. Phytoparasitica 34, 516–522 (2006). https://doi.org/10.1007/BF02981208

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981208

Key words

Navigation