Abstract
Some species of the genusOrobanche are among the most devastating parasitic weeds, causing extensive damage in agricultural fields. Considering the difficult control due to seed longevity in the soil, small seed size, high fecundity and a subterranean phase that allows them to parasitize the host before they emerge and become evident, the development of diagnostic markers is highly recommended. In our study we identified potential molecular diagnostic markers from the plastid genome in order to distinguish among the most importantOrobanche species attacking crops in Andalusia, the southern region of the Iberian Peninsula. The study has consideredO. crenata, O ramosa andO. cumana causing serious losses in legumes, solanaceous crops and sunflower fields, respectively, andO. minor that, although abundant in Andalusia, has to our knowledge not yet been found parasitizing agricultural hosts. We amplified a non-coding region from the plastid genome, studied sequence differences among the amplified fragments and digested those of the same length with selected restriction enzymes. Here, we propose a molecular protocol to distinguish the main parasitic plants in crop fields of southern Spain. Different applications such as identification ofOrobanche seeds in soil or crop seed lots are discussed in order to offer right crop recommendations or to prevent new infestation of parasite-free fields. Recommendations for further development of these diagnostic markers are also considered.
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References
Benharrat, H., Delavault, P., Theodet, C., Figureau, C. and Thalouarn, P. (2000) rbcL Plastid pseudogene as a tool forOrobanche (subsectionMinores) identification.Plant Biol. 2:34–39.
Cubero, J.I. (1991) Breeding for resistance toOrobanche species: a review.in: Wegmann, K. and Musselman, L.J. [eds.] Progress inOrobanche Research. Eberhard-Karls-Universitat, Tübingen, Germany. pp. 257–277.
Delavault, P. and Thalouarn, P. (2002) The obligate root parasiteOrobanche cumana exhibits several rbcL sequences.Gene 297:85–92.
Demesure, B., Sodzi, N. and Petit, R.J. (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants.Mol. Ecol. 4:129–131.
dePamphilis, C.W., Young, N.D. and Wolfe, A.D. (1997) Evolution of plastid generps2 in a lineage of hemiparasitic and holoparasitic plants: Many losses of photosynthesis and complex patterns of rate variation.Proc. Natl. Acad. Sci. USA 94:7367–7372.
Joel, D.M., Portnoy, V.H. and Katzir, N. (1998) Use of DNA fingerprinting for soil-borne seed identification.Aspect Appl. Biol. 51:23–27.
Lassner, M.W., Peterson, P. and Yoder, J.I. (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny.Plant Mol. Biol. Rep. 7:116–128.
López-Granados, F. and García-Torres, L. (1999) Longevity of crenate broomrape (Orobanche crenata) seed under soil and laboratory conditions.Weed Sci. 47:161–166.
Nickrent, D.L., Duff, R.J., Colwell, A.E., Wolfe, A.D., Young, N.D., Steiner, K.E.et al. (1998) Molecular phylogenetic and evolutionary studies of parasitic plants.in: Soltis, D., Soltis, P. and Doyle, J. [Eds.] Molecular Systematics of Plants II: DNA Sequencing. Kluwer Academic Publishers, Boston, MA, USA. pp. 211–241.
Osterbauer, N.K. and Rehms, L. (2002) Detecting single seeds of small broomrape (Orobanche minor) with a polymerase chain reaction. Online.Plant Health Progress doi: 10.1094/PHP-2002-1111-01-RS.
Parker, C. and Riches, C.R. (1993) Parasitic Weeds of the World: Biology and Control. CAB International, Wallingford, UK.
Portnoy, V.H., Katzir, N., Aly, R. and Joel, D. (1998) Use of Sequence Characterized Amplified DNA regions (SCAR) for species identification of soil-borneOrobanche seeds in agricultural fields.Proc. 6 th Mediterranean Symp. EWRS (Montpellier, France), p. 158.
Portnoy, V.H., Katzir, N. and Joel, D.M. (1997) Species identification of soil-borneOrobanche seeds by DNA fingerprinting.Pestic. Biochem. Physiol. 58:49–54.
Rehms, L. and Osterbauer, N.K. (2003) DetectingOrobanche minor seeds in soil using PCR. Online.Plant Health Progress doi: 10.1094/PHP-2003-0701-01-HN.
Schneeweiss, G.M., Colwell, A., Park, J.M., Jang, C.G. and Stuessy, T.F. (2004) Phylogeny of holoparasiticOrobanche (Orobanchaceae) inferred from nuclear ITS sequences.Mol. Phylogenet. Evol. 30:465–478.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucl. Acids Res. 25:4876–4882.
Torres, A.M., Weeden, N.F. and Martín, A. (1993) Linkage among isozyme, RFLP and RAPD markers inVicia faba.Theor. Appl. Genet. 85:937–945.
Wolfe, A.D. and dePamphilis, C.W. (1997) Alternate paths of evolution for the photosynthetic gene rbcL in four non-photosynthetic species ofOrobanche.Plant Mol. Biol. 33:965–977.
Wolfe, A.D. and dePamphilis, C.W. (1998) The effect of relaxed functional constraints on the photosynthetic gene rbcL in parasitic plants of Scrophulariales.Mol. Biol. Evol. 15:1243–1258.
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http://www.phytoparasitica.org posting Jan. 15, 2007.
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Román, B., González Verdejo, C.I., Satovic, Z. et al. DetectingOrobanche species by using cpDNA diagnostic markers. Phytoparasitica 35, 129–135 (2007). https://doi.org/10.1007/BF02981106
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DOI: https://doi.org/10.1007/BF02981106