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Characterization of five Indian isolates of the nuclear polyhedrosis virus ofHelicoverpa armigera

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Abstract

Five Indian geographical isolates of the nuclear polyhedrosis virus ofHelicoverpa (Heliothis) armigera were studied for the variation in their size, virulence, and the restriction and electrophoretic patterns of the polyhedrin. The diameter of the polyhedra varied from 1.6 to 2.4 μm (mean 1.933 μm), with no significant differences among the five isolates. The most virulent isolate was that from Ooty (OOT), with the lowest LC50 value of 2.538 × 103 purified occlusion bodies (POB)/ml, followed by the isolate from Coimbatore (CBE). Genomic variability as determined by the HindIII digestion pattern of the DNA, showed that isolates OOT and CBE were similar and that the three other isolates,viz., APK, GUJ, and RAJ represented another group which had a similar restriction pattern. The SDS-PAGE electrophoretic pattern of the polyhedrin of all the isolates was the same.

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Authors and Affiliations

  1. Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, 641 003, Coimbatore, India

    R. J. Rabindra

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  1. S. Somasekar
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  2. M. Jayapragasam
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  3. R. J. Rabindra
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Somasekar, S., Jayapragasam, M. & Rabindra, R.J. Characterization of five Indian isolates of the nuclear polyhedrosis virus ofHelicoverpa armigera . Phytoparasitica 21, 333–337 (1993). https://doi.org/10.1007/BF02981052

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