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Comparison ofPotato Virus Y andPlum Pox Virus transmission by two aphid species in relation to their probing behavior

  • Virology/Entomology
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Abstract

Two different aphid species,Myzus persicae (Sulzer) andHyalopterus pruni (Geoffroy) (Homoptera: Aphididae), were used to analyze their ability to transmit two different potyviruses,Potato virus Y (PVY) andPlum pox virus (PPV), to pepper (Capsicum annuum) andNicotiana benthamiana plants, respectively. In parallel experiments,M. persicae consistently transmitted both viruses with high efficiency, whereasH. pruni always failed to transmit either virus. This is in contrast to previous reports describingH. pruni as a vector of these potyviruses. Different aphid probing behavior among individual aphids of each species was obtained in electrical penetration graph (EPG) experiments performed on pepper plants. This suggested thatH. pruni did not transmit these potyviruses due to behavioral differences during probing that impeded virus acquisition and/or inoculation. It was found thatM. persicae usually makes its first probe within the first 2 min, whereasH. pruni individuals remained for more than 10 min on the plant before starting to probe. Furthermore,M. persicae individuals displayed their first intracellular puncture during the first minute of probing whereasH. pruni needed ∼ 15 min to penetrate the cell plasmalemma with their stylets. In addition, intracellular stylet punctures occurred very frequently forM. persicae but was a rare event, never exceeding a single one, forH. pruni. The relevance of these findings for the epidemiological spread of potyviruses by different aphid species is discussed.

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References

  1. Abacus Concepts (1992) Statview. Abacus Concepts Inc., Berkeley, CA, USA.

    Google Scholar 

  2. Aboul-Ela, A., Aboul-Ata, E. and Mazyad, H.M. (1999) Plum pox potyvirus in Egyptian stone fruit trees.Acta Hortic. 488:745–751.

    Google Scholar 

  3. Chen, J.Q., Martín, B., Rahbé, Y. and Fereres, A. (1997) Early intracellular punctures of two aphids on nearisogenic melon lines with and without the virus aphid transmission (Vat) resistance gene.Eur. J. Plant Pathol. 103:521–536.

    Article  Google Scholar 

  4. Elíbüyük, Í.Ö. (2003) Natural spread ofPlum pox virus in Ankara, Turkey.J. Phytopathol. 151:617–619.

    Article  Google Scholar 

  5. Febvay, G., Rahbé, Y. and van Helden, M. (1996) MacStylet, software to analyse electrical penetration graph data on the Macintosh.Entomol. Exp. Appl. 80:105–108.

    Article  Google Scholar 

  6. Fereres, A. and Collar, J.L. (2001) Analysis of noncirculative transmission by electrical penetration graphs.in: Harris, K.F., Smith, O.P. and Duffus, J.E. [Eds.] Virus — Insect — Plant Interactions. Academic Press, San Diego, CA, USA.

    Google Scholar 

  7. Garzo, E.I., Duque, M. and Fereres, A. (2004) Transmission efficiency of different non-persistent viruses infecting melon by four aphid species.Span. J. Agric. Res. 2:369–376.

    Google Scholar 

  8. Gibson, R.W., Payne, R.W. and Katis, N. (1988) The transmission of patato virus Y by aphids of different vectoring abilities.Ann. Appl. Biol. 113:35–43.

    Article  Google Scholar 

  9. Harrington, R. and Gibson, R.W. (1989) Transmission of potato virus Y by aphids trapped in potato crops in southern England.Potato Res. 32:167–174.

    Article  Google Scholar 

  10. Harrington, R., Katis, N. and Gibson, R.W. (1986) Field assessment of the relative importance of different aphid species in the transmission of potato virus Y.Potato Res. 29:67–76.

    Article  Google Scholar 

  11. Isac, M., Preda, S. and Marcu, M. (1998) Aphid species — vectors of plum pox virus.Acta Virol. 42:233–234.

    PubMed  CAS  Google Scholar 

  12. Katis, N. and Gibson, R.W. (1985) Transmission of potato virus Y by cereal aphids.Potato Res. 28:65–70.

    Article  Google Scholar 

  13. Kirk, M., Temple, S.R., Summers, C.G. and Wilson, L.T. (1991) Transmission efficiencies of field-collected aphid (Homoptera: Aphididae) vectors of beet yellows virus.J. Econ. Entomol. 84:638–643.

    Google Scholar 

  14. López-Moya, J.J., Canto, T., Díaz-Ruíz, J.R. and López-Abella, D. (1995) Transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus Y helper component.J. Gen. Virol. 76:2293–2297.

    Article  PubMed  Google Scholar 

  15. Martin, B., Collar, J.L., Tjallingii, W.F. and Fereres, A. (1997) Intracellular ingestion and salivation by aphids may cause the acquisition and inoculation of non-persistently transmitted plant viruses.J. Gen. Virol. 78:2701–2705.

    PubMed  CAS  Google Scholar 

  16. Minoiu, N., Maxim, A., Vladianu, D., Platon, I. and Balaci, R. (1998) New results concerning the plum pox virus epidemiology and resistance of plum cultivars, hybrids and rootstocks.Acta Virol. 42:244–247.

    PubMed  CAS  Google Scholar 

  17. Moericke, V. (1969) Hostplant specific colour behaviour byHyalopterus pruni (Aphididae).Entomol. Exp. Appl. 12:524–534.

    Article  Google Scholar 

  18. Perez, P., Collar, J.L., Avilla, C., Duque, M. and Fereres, A. (1995) Estimation of vector propensity of Potato Virus Y in open-field pepper crops of central Spain.J. Econ. Entomol. 88:986–991.

    Google Scholar 

  19. Perring, T.M., Gruenhagen, N.M. and Farrar, C.A. (1999) Management of plant viral diseases through chemical control of insect vectors.Annu. Rev. Entomol. 44:457–481.

    Article  PubMed  CAS  Google Scholar 

  20. Peters, D., Brooijmans, E. and Grondhuis, P.F.M. (1990) Mobility as a factor in the efficiency with which aphids can spread non-persistently transmitted viruses.Proc. Exp. Appl. Entomol. 1:190–194.

    Google Scholar 

  21. Piron, P.G.M. (1986) New aphid vectors of potato virus YN.Neth. J. Plant Pathol. 92:223–229.

    Article  Google Scholar 

  22. Powell, G. (1991) Cell membrane punctures during epidermal penetration by aphids: consequences for the transmission of two potyviruses.Ann. Appl. Biol. 119:313–321.

    Article  Google Scholar 

  23. Powell, G. (2005) Intracellular salivation is the aphid activity associated with inoculation of non-persistently transmitted viruses.J. Gen. Virol. 86:469–472.

    Article  PubMed  CAS  Google Scholar 

  24. Powell, G., Pirone, J. and Hardie, J. (1995) Aphid stylet activities during potyvirus acquisition from plants and anin vitro system that correlate with subsequent transmission.Eur. J. Plant Pathol. 101:411–420.

    Article  Google Scholar 

  25. Raccah, B., Gal-On, A. and Eastop, V.F. (1985) The role of flying aphid vectors in the transmission of cucumber mosaic virus and potato virus Y to peppers in Israel.Ann. Appl. Biol. 106:451–460.

    Article  Google Scholar 

  26. Ruiz-Ferrer, V., Goytia, E., Martinez-Garcia, B., Lopez-Abella, D. and Lopez-Moya, J.J. (2004) Expression of functionally active helper component protein ofTobacco etch potyvirus in the yeastPichia pastoris.J. Gen. Virol. 85:241–249.

    Article  PubMed  CAS  Google Scholar 

  27. Sigvald, R. (1984) The relative efficiency of some aphid species as vectors of potato virus YO (PVYO).Potato Res. 27:285–290.

    Article  Google Scholar 

  28. Sigvald, R. (1987) Aphid migration and the importance of some aphid species as vectors of potato virus Yo (PVYo) in Sweden.Potato Res. 30:267–283.

    Article  Google Scholar 

  29. Sigvald, R. (1989) Relationship between aphid occurrence and spread of potato virus Yo (PVYo) in field experiments in southern Sweden.J. Appl. Entomol. 108:35–43.

    Article  Google Scholar 

  30. Tjallingii, W.F. (1985) Membrane potentials as an indication for plant cell penetration by aphid stylets.Entomol. Exp. Appl. 38:187–195.

    Article  Google Scholar 

  31. Tjallingii, W.F. (1990) Continuous recording of stylet penetration activities by aphids.in: Campbell, R.K. and Eikenbary, R.D. [Eds.] Aphid — Plant Genotype Interactions. Elsevier, Amsterdam, the Netherlands. pp. 89–99.

    Google Scholar 

  32. van Hoof, H.A. (1980) Aphid vectors of potato virus YN.Neth. J. Plant Pathol. 86:159–162.

    Article  Google Scholar 

  33. Wang, R.Y. and Ghabrial, S.A. (2002) Effect of aphid behavior on efficiency of transmission ofSoybean mosaic virus by the soybean-colonizing aphid,Aphis glycines.Plant Dis. 86:1260–1264.

    Article  Google Scholar 

  34. Wang, R.Y., Powell, G., Hardie, J. and Pirone, T.P. (1998) Role of the helper component in vector-specific transmission of potyviruses.J. Gen. Virol. 79:1519–1524.

    PubMed  CAS  Google Scholar 

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Author notes

  1. Juan José López-Moya

    Present address: Consorcio CSIC-IRTA, IBMB, CSIC, 08035, Barcelona, Spain

Authors and Affiliations

  1. Dpto. de Biología de Plantas, CIB, CSIC, 28040, Madrid, Spain

    Lourdes Fernández-Calvino, Dionisio López-Abella & Juan José López-Moya

  2. Dpto. de Protección Vegetal, CCMA, CSIC, 28006, Madrid, Spain

    Alberto Fereres

Authors
  1. Lourdes Fernández-Calvino
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  2. Dionisio López-Abella
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  3. Juan José López-Moya
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  4. Alberto Fereres
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Correspondence to Alberto Fereres.

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http://www.phytoparasitica.org posting May 14, 2006.

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Fernández-Calvino, L., López-Abella, D., López-Moya, J.J. et al. Comparison ofPotato Virus Y andPlum Pox Virus transmission by two aphid species in relation to their probing behavior. Phytoparasitica 34, 315–324 (2006). https://doi.org/10.1007/BF02980959

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  • DOI: https://doi.org/10.1007/BF02980959

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