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PCR-RFLP identification ofBemisia tabaci biotypes in the Mediterranean Basin

  • Entomology
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Abstract

At least 20 genetic biotypes, with varying degrees of biological characterization, are currently recognized within theBemisia tabaci (Hemiptera: Aleyrodidae) species complex. Their identification relies on a set of different molecular techniques. However, none of the available markers is completely adequate, due to technical difficulties or lack of reproducibility. We therefore developed a method for rapid biotyping ofB. tabaci populations. The five biotypes (B, Q, M, S and T) reported until now in the Mediterranean Basin have been tested by PCR amplification of the cytochrome oxidase I mitochondrial gene followed by restriction with the enzymeTru9I. The digestion patterns produced by this enzyme were able to identify the five biotypes clearly. Digestion with another enzyme,TaqI, discriminated only between biotypes B and Q. The newly developed method enables rapid biotyping and can be applied in studies aimed at assessing biotype distribution and competition at least in the Mediterranean area.

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Authors and Affiliations

  1. Di. Va.P.R.A. - Entomologia e Zoologia applicate all’Ambiente, Università di Torino, 10095, Grugliasco (TO), Italy

    Domenico Bosco, Andrea Loria & Chiara Sartor

  2. IMIDA, c/ Mayor sn, 30150, La Alberca (Murcia), Spain

    José Luis Cenis

Authors
  1. Domenico Bosco
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  2. Andrea Loria
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  3. Chiara Sartor
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  4. José Luis Cenis
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Correspondence to Domenico Bosco.

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http://www.phytoparasitica.org May 14, 2006.

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Bosco, D., Loria, A., Sartor, C. et al. PCR-RFLP identification ofBemisia tabaci biotypes in the Mediterranean Basin. Phytoparasitica 34, 243–251 (2006). https://doi.org/10.1007/BF02980951

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  • DOI: https://doi.org/10.1007/BF02980951

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