Skip to main content
SpringerLink
Log in

Sensitivity ofFusarium graminearum to fungicide JS399-19:In vitro determination of baseline sensitivity and the risk of developing fungicide resistance

  • Phytopathology
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

The baseline sensitivity ofFusarium graminearum Schwade [teleomorph =Gibberella zeae (Schweinitz) Petch] to the fungicide JS399-19 (development code no.) [2-cyano-3-amino-3-phenylacrylic acetate] and the assessment of risk to JS399-19 resistancein vitro are presented. The mean EC50 values for JS399-19 inhibiting mycelial growth of three populations of wild-typeF. graminearum isolates were 0.102±0.048, 0.113±0.035 and 0.110±0.036 µg ml−1, respectively. Through UV irradiation and selection for resistance to the fungicide, we obtained a total of 76 resistant mutants derived from five wild-type isolates ofF. graminearum with an average frequency of 1.71 × 10−7% and 3.5%, respectively. These mutants could be divided into three categories of resistant phenotypes with low (LR), moderate (MR) and high (HR) level of resistance, determined by the EC50 values of 1.5–15.0 µg ml−1, 15.1–75.0 µg ml−1 and more than 75.0 µg ml−1, respectively. There was no positive cross-resistance between JS399-19 and fungicides belonging to other chemical classes, such as benzimidazoles, ergosterol biosynthesis inhibitors and strobilurins, suggesting that JS399-19 presumably has a new biochemical mode of action. Although the resistant mutants appeared to have comparable pathogenicity to their wild-type parental isolates, they showed decreased mycelial growth on potato-sucrose-agar plates and decreased sporulation capacity in mung bean broth. Nevertheless, most of the resistant mutants possessed fitness levels comparable to their parents and had MR or HR levels of resistance. As these studies yielded a high frequency of laboratory resistance inF. graminearum, appropriate precautions against resistance development in natural populations should be taken into account.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Anon. (1982) Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides.FAO Plant Prot. Bull. 30(2):30–36.

  2. Bai, G., and Shaner, G. (1994) Scab of wheat: prospects for control.Plant Dis. 78:760–766.

    Google Scholar 

  3. Bollen, G.J. and Scholten, G. (1971) Acquired resistance to benomyl and some other systemic fungicides in a strain ofBotrytis cinerea in cyclamen.Neth. J. Plant Pathol. 77:83–90.

    Article  CAS  Google Scholar 

  4. Bowden, R.L. and Leslie, J.F. (1999) Sexual recombination inGibberella zeae.Phytopathology 89:182–188.

    Article  PubMed  Google Scholar 

  5. Broders, K.D., Lipps, P.E., Paul, P.A. and Dorrance, A.E. (2007) Evaluation ofFusarium graminearum associated with corn and soybean seed and seedling disease in Ohio.Plant Dis. 91:1155–1160.

    Article  Google Scholar 

  6. Carter, J.P., Rezanoor, H.N., Holden, D., Desjardins, A.E., Plattner, R.D. and Nicholson, P. (2002) Variation in pathogenicity associated with the genetic diversity ofFusarium graminearum.Eur. J. Plant Pathol. 108:573–583.

    Article  CAS  Google Scholar 

  7. Casale, W.L. and Hart, L.P. (1988) Inhibition of 3H-leucine incorporation by trichothecene mycotoxins in maize and wheat tissue.Phytopathology 78:1673–1677.

    Article  CAS  Google Scholar 

  8. Chen, L.F., Bai, G.H. and Desjardins, A.E. (2000) Recent advances in wheat head scab research in China. National Agricultural Library. (http://www.nal.usda.gov/pgdic/WHS/whsindex.html)

  9. Forsyth, O.M., Yoshizawa, T., Morooka, N. and Tuite, J. (1977) Emetic and refusal activity of deoxynivalenol to swine.Appl. Environ. Microbiol. 34:547–552.

    PubMed  CAS  Google Scholar 

  10. Ishii, H. (2006) Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment.Jpn. Agric. Res. Q. 40(3):205–211. (http://www.jircas.affrc.go.jp/)

    CAS  Google Scholar 

  11. Karaoglanidis, G.S., Ioannidis, P.M. and Thanassoulopoulos, G.C. (2002) Changes in sensitivity ofCercospora beticola populations to steroldemethylation-inhibiting fungicides during a 4-year period in northern Greece.Plant Pathol. 51:55–62.

    CAS  Google Scholar 

  12. Klittich, C.J.R. and Leslie, J.F. (1988) Nitrate reduction mutants ofFusarium moniliforme (Gibberella fujikuroi).Genetics 118:417–423.

    PubMed  CAS  Google Scholar 

  13. Klix, M.B., Verreet, J.A. and Beyer, M. (2007) Comparison of the declining triazole sensitivity ofGibberella zeae and increased sensitivity achieved by advances in triazole fungicide development.Crop Prot. 26:683–690.

    Article  CAS  Google Scholar 

  14. Li, H.K., Diao, Y., Wang, J.X., Chen, C., Ni, J. and Zhou, M. (2008) JS399-19, a new fungicide against wheat scab.Crop Protection 27:90–95.

    Article  CAS  Google Scholar 

  15. McMullen, M.P., Jones, R. and Gallenberg, D. (1997) Scab of wheat and barley: a re-emerging disease of devastating impact.Plant Dis. 81:1340–1348.

    Article  Google Scholar 

  16. Nash, S.M. and Snyder, W.C. (1962) Quantitative estimation by plate counts of propagules of the bean root rot Fusarium in field soils.Phytopathology 52:567–572.

    Google Scholar 

  17. Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O. (1983)Fusarium species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park, PA, USA.

    Google Scholar 

  18. Proctor, R.H., Hohn, T.M. and McCormick, S.P. (1995) Reduced virulence ofGibberella zeae caused by disruption of trichothecene toxin biosynthesis gene.Mol. Plant-Microbe Interact. 8:593–601.

    PubMed  CAS  Google Scholar 

  19. Schroeder, K.L. and Provvidenti, R. (1969) Resistance to benomyl in powdery mildew of cucurbits.Plant Dis. Rep. 53:326–333.

    Google Scholar 

  20. Semaškiené, R., Mankevičienė, A., Dabkevičius, Z. and Supronienė, S. (2006) Effect of fungicides onFusarium infection and production of deoxynivalenol in spring cereals.Agron. Res. 4:363–366.

    Google Scholar 

  21. Snijders, C.H.A. (1990) Fusarium head blight and mycotoxin contamination of wheat, a review.Neth. J. Plant Pathol. 96:187–198.

    Article  CAS  Google Scholar 

  22. Stoyan, R.P., Simon, G.E., Martin, C.H. and Peter, J. (2003) Strategies for the control of Fusarium head blight in cereals.Eur. J. Plant Pathol. 109:731–742.

    Article  Google Scholar 

  23. Suty, A. and Stenzel, K. (1999) Iprovalicarb-sensitivity ofPhytophthora infestans andPlasmopara viticola: determination of baseline sensitivity and assessment of the risk of resistance.Pflanzenschutz-Nachr. Bayer 52:171–182.

    Google Scholar 

  24. Taylor, R.J., Sales, B., Secor, G.A., Rivera, V. and Gudmestad, N.C. (2002) Sensitivity of North American isolates ofPhytophthora erythroseptica andPythium ultimum to mefenoxam (metalaxyl).Plant Dis. 86:797–802.

    Article  CAS  Google Scholar 

  25. Tomohiro, B. and Kazuhiro, S. (2000) Genetic analysis of resistance to Fusarium head blight caused byFusarium graminearum in Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165.Euphytica 113:87–99.

    Article  Google Scholar 

  26. Vesonder, R.F., Ciegler, A., Jensen, A.H., Rohwedder, W.K. and Weislander, D. (1976) Co-identity of the refusal and emetic principle from Fusarium-infected corn.Appl. Environ. Microbiol. 31:280–285.

    PubMed  CAS  Google Scholar 

  27. Wang, J.X. and Zhou, M.G. (2002) Methods for monitoring resistance ofGibberella zeae to carbendazim.Acta Phytophylactica Sin. 29:73–77.

    CAS  Google Scholar 

  28. Wicks, T. (1974) Tolerance of the apple scab fungus to benzimidazole fungicides.Plant Dis. Rep. 58:886–889.

    CAS  Google Scholar 

  29. Zhou, M.G. and Wang, J.X. (2001) Study on sensitivity base-line ofFusarium graminearum to carbendazim and biological characters of MBC-resistant isolates.Acta Phytopathol. Sin. 31:365–370.

    Google Scholar 

  30. Zhou, M.G., Wang, J.X., Lu, Y.J., Ju, L.H. and Ye, Z.Y. (1994) Study on mutation of resistance inFusarium graminearum to carbendazim.J. Nanjing Agric. Univ. 17 (suppl.):106–112.

    Google Scholar 

  31. Zhou, M.G., Ye, Z.Y. and Liu, J.F. (1994) Progress of fungicide resistance.J. Nanjing Agric. Univ. 17(3):33.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, China

    Yu Chen, Changjun Chen & Mingguo Zhou

  2. Dept. of Textile of Apparel, Anhui University of Technology and Science, 241000, Wuhu, China

    Hengkui Li

Authors
  1. Yu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hengkui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Changjun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mingguo Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingguo Zhou.

Additional information

http://www.phytoparasitica.org posting August 7, 2008.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, Y., Li, H., Chen, C. et al. Sensitivity ofFusarium graminearum to fungicide JS399-19:In vitro determination of baseline sensitivity and the risk of developing fungicide resistance. Phytoparasitica 36, 326–337 (2008). https://doi.org/10.1007/BF02980812

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02980812

Key words

Search

Navigation