Abstract
The kinetics of accumulation of tobacco mosaic virus (TMV) and the S strain of pepper mild mottle virus (PMMV) in resistant and susceptible pepper cultivars was analyzed by enzyme-linked immunosorbent assay (ELISA). Crude protein extracts from noninoculated leaves of the resistant cultivar significantly inhibited TMV replication in tobacco protoplasts. Inoculation with either TMV or PMMV-S caused a slight increase in its inhibitory potency. In contrast, protein extracts from either inoculated or noninoculated leaves of the susceptible cultivar did not inhibit TMV replication in tobacco protoplasts. The protein extracts from the resistant cultivar gave a positive reaction with antibodies against the tobacco inhibitor of virus replication (IVR) in western blotting. The putative role of this IVR-like protein from the resistant cultivar in resistance against TMV is discussed.
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Alonso, E., García-Luque, I., Avila Rincon, J., Wicke, B., Serra, M.T. and Diaz-Ruiz, J.R. (1989) A tobamovirus causing heavy losses in protected pepper crops in Spain.J. Phytopathol. 125: 67–76.
Avrameas, S. (1969) Coupling of enzymes to proteins with glutaraldehyde.Immunochemistry 6:43–53.
Boukema, I.W. (1984) Resistance to TMV inCapsicum chacoense Hunz. is governed by an allele of the L-locus.Capsicum Newsl. 3:47–48.
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72: 248–254.
Brunt, A.A. (1986) Miscellaneous tobamoviruses.in: van Regenmortel, M.H.V. and Fraenkel-Conrat, H. [Eds.] The Plant Viruses. Plenum Press, New York, NY. Vol. 2, pp. 283–302.
Clark, M.F. and Adams, A.N. (1977) Characteristics of the microplate method of enzymelinked immunosorbent assay for the detection of plant viruses.J. Gen. Virol. 34:475–483.
García-Luque, I., Ferrero, M.L., Rodriguez, J.M., Alonso, E., De La Cruz, A., Sanz, A.I., Vaquero, C., Serra, M.T. and Diaz-Ruiz, J.R. (1993) The nucleotide sequence of the coat protein genes and 3 ’ non-coding regions of two resistance-breaking tobamoviruses in pepper shows that they are different viruses.Arch. Virol. 131: 75–88.
García-Luque, I., Serra, M.T., Alonso, E., Wicke, B., Ferrero, M.L. and Diaz-Ruiz, J.R. (1990) Characterization of a Spanish strain of pepper mild mottle virus (PMMV-S) and its relationship to other tobamoviruses.J. Phytopathol. 129:1–8.
Gera, A. and Loebenstein, G. (1983) Further studies of an inhibitor of virus replication from tobacco mosaic virus-infected protoplasts of a local lesion-responding tobacco cultivar.Phytopathology 73:111–115.
Gera, A., Loebenstein, G., Salomon, R. and Frank, A. (1990) Inhibitor of virus replication (IVR) from protoplasts of a hypersensitive tobacco cultivar infected with tobacco mosaic virus, is associated with a 23K protein species.Phytopathology 80: 78–81.
Konate, G., Kopp, M. and Fritig, B. (1982) Multiplication du virus de la mosaïque du tabac dans des hôtes à réponse systémique ou nécrotique: Approche biochimique à l ’étude de la résistance hypersensible aux virus.Phytopathol. Z. 105: 214–225.
Loebenstein, G. and Gera, A. (1981) Inhibitor of virus replication released from tobacco mosaic virus-infected protoplasts of a local lesion responding tobacco cultivar.Virology 114:132–139.
Loebenstein, G. and Gera, A. (1990) Inhibitor of virus replication associated with resistance responses.in: Fraser, R.S.S. [Ed.] Recognition and Response in Plant-Virus Interactions. NATO ASI Series. Vol. 441. Springer Verlag, Berlin, pp. 395–403.
Loebenstein, G., Gera, A. and Gianinazzi, S. (1990) Constitutive production of an inhibitor of virus replication in the interspecific hybrid ofNicotiana glutinosa x Nicotiana debneyi.Physiol. Mol. Plant Pathol. 37: 145–151.
Morrissey, J.A. (1981) Silver staining for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.Anal. Biochem. 117: 307–310.
Otsuki, Y., Shimomura, T. and Takebe, I. (1972) Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties.Virology 50: 45–50.
Spiegel, S., Gera, A., Salomon, R., Ahl, P., Harlap, S. and Loebenstein, G. (1989) Recovery of an inhibitor of virus replication from the intercellular fluid of hypersensitive tobacco infected with tobacco mosaic virus and from uninfected induced-resistant tissue.Phytopathology 79: 258–262.
Tostado, J.M., Wicke, B., García-Luque, I., Diaz-Ruiz, J.R. and Serra, M.T. (1990) Pathogenesis related (PR) proteins in pepper plants infected with pepper mild mottle virus (PMMV-S) and tobacco mosaic virus (TMV).Abstr. VIIIth Int. Congr. Virology (Berlin, Germany), p. 461.
Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications.Proc. Natl. Acad. Sci. USA 76: 4350–4354.
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Gera, A., Tostado, J.M., García-Luque, I. et al. Association of the inhibitor of virus replication with resistance mechanisms in pepper cultivars. Phytoparasitica 22, 219–227 (1994). https://doi.org/10.1007/BF02980321
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DOI: https://doi.org/10.1007/BF02980321