Abstract
Toxic products extracted by methanol from intact mycelia ofVerticillium lecanii were tested for their toxicity to eggs, larvae, pupae and adults ofBemisia tabaci and additional insects. All stages ofB. tabaci were sensitive to the toxin, with larvae showing the highest, and adults the lowest, sensitivity. Using a 0.5% crude toxin preparation, the mortality rate ofB. tabaci was 88%, 53.5%, 53.2% and 37% for larvae, eggs, pupae and adults, respectively. High sensitivity of adults, which was characterized by rapid paralysis followed by death, was observed inB. tabaci, Myzus persicae, Aphis gossypii, Acyrthosiphon pisum andFrankliniella occidentalis. Other insects,viz., Heliothis peltigera, Maladera matrida, Carpophilus hemipterus andCeratitis capitata, exhibited significantly lower sensitivity. The toxin was partially purified by differential extraction with organic solvents and on silica gel H and Sephadex LH 20 columns. Based on the toxin inactivation by saponification and its mobility in a polar solvent on thin layer chromatography, it is suggested that the toxic products might be phospholipids.
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References
Baird, R.B. (1954) A species ofCephalosporium (Moniliaceae) causing a fungus disease in larvae of the European corn borer,Pyrausta nubilalis (Hbn.) (Lepidoptera: Pyraustidae).Can. Entomol. 86:237–240.
Conant, N.F., Smith, D.T., Baker, R.D. and Callawag, L.J. (1971) Manual of Clinical Mycology. W.B. Saunders Company, New York, NY.
Gindin, G., Singer, S., Raccah, B. and Barash, I. (1992) Application of extracts fromVerticillium lecanii for the control of three vector species of virus diseases.Proc. Annual Meeting of the Israeli Society for Microbiology (Be ’er Sheva, Israel), p. 26.
Gindina, G.M., Mitina, G.V. and Pavlyushin, V.A. (1990) [Toxigenicity of natural isolates ofVertkillium lecanii (Zimmerman) Viegas.]Mycol. Phytopathol. 24:576–582 (in Russian).
Hall, R.A. (1976)Verticillium lecanii on the aphidMacrosiphoniella sanborni.J. Invertebr. Pathol. 28:389–391.
Hall, R.A. (1981) The fungusVerticillium lecanii as a microbial insecticide against aphids and scales,in: Burges, H.D. [Ed.] Microbial Control of Pests and Plant Diseases 1970–1980. pp. 483–498. Academic Press, New York, NY.
Kanaoka, M., Isogai, A., Murakoshi, S., Ichinoe, M., Suzuki, A. and Tamura, S. (1978) Bassianolide, a new insecticidal cyclodepsipeptide fromBeauveria bassiana andVertkillium lecanii.Agric. Biol. Chem. 42:629–635.
Mitina, G.V. (1992) Insecticidal toxins ofVerticillium lecanii (Zimm.) Viegas, producer of biopreparation “Verticillin ”. Doctoral thesis, Ail-Russian Institute of Plant Protection, St. Petersburg, Russia. 18 pp.
Roberts, D.W. (1981) Toxins of entomopathogenic fungi.in: Burges, H.D. [Ed.] Microbial Control of Pests and Plant Diseases 1970–1980. pp. 441–464. Academic Press, New York, NY.
USSR Patent, (1984) Biological insecticide for pest control of aphids. Patent No. 1221777.
Voronina, E.G. and Gindina, G.M. (1986) Character of parasite-host interactions by changing of nutrition model for entomophthorous fungi.in: Abstracts of Reports for the Scientific Conference URNOP (Kiev, USSR), p. 124.
Wasti, S.S. and Hartman, G.C. (1978) Host-parasite interactions between larvae of the gypsy moth,Lymantria dispar (L.) (Lepidoptera: Lymantridae), and the entomogenous fungus,Nomuraea rileyi (Farlow) Samson (Moniliales: Moniliaceae).Appl. Entomol. Zool. 13:23–28.
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Gindin, G., Barash, I., Harari, N. et al. Effect of endotoxic compounds isolated fromVerticillium lecanii on the sweetpotato whitefly,Bemisia tabaci . Phytoparasitica 22, 189–196 (1994). https://doi.org/10.1007/BF02980318
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DOI: https://doi.org/10.1007/BF02980318