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Antioxidant activities of isoflavones from the rhizomes ofbelamcanda chinensis on carbon tetrachloride-lnduced hepatic injury in rats

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Abstract

The present study was carried out to clarify whether tectorigenin and tectoridin isolated from the rhizomes ofBelamcanda chinensis (Iridaceae) inhibit hepatic damage induced by carbon tetrachloride (CCI+4)-intoxication in rats by the experimental methodsin vitro andin vivo. Tectorigenin and tectoridin exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by CCI4-intoxication in rats, as well as in a lipid peroxidation causing a significant decrease in malondialdehyde (MDA) production by thiobarbituric acid (TBA)-reactant assay. Both compounds also showed strong increase in the antioxidant enzymes such as hepatic cytosolic superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-px) activities in CCI4-intoxicated rats. These results suggested that tectorigenin and tectoridin isolated from the rhizomes ofB. chinensis possess not only the antioxidative, but also the hepatoprotective activities in CCI4-intoxicated rats.

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References

  • Beckman, K. B. and Ames, B. N., The free radical theory of aging matures.Physiol. Rev., 78, 547–581 (1998).

    PubMed  CAS  Google Scholar 

  • Brar, S. S., Kennedy, T. P., Sturrock, A. B., Huecksteadt, T. P., Quinn, M. T., Murphy, T. M., Chitano, P., and Hoidal, J. R., NADPH oxidase promotes NF-kB activation and proliferation in human airway smooth muscle.Am. J. Physiol. Lung Cell Mol. Physiol., 282, L782-L795 (2002).

    PubMed  CAS  Google Scholar 

  • Buege, J. A. and Aust, S. D., Microsomal lipid peroxidation. Method Enzymol., 52, 302–306 (1978).

    Article  CAS  Google Scholar 

  • Burk, R. F., Nishiki, K., Lawrence, R. A., and Chance, B., Peroxide removal by selenium-dependent and seleniumindependent glutathione peroxidases in hemoglobin free perfused rat liver.J. Biol. Chem., 253, 43–46 (1978).

    PubMed  CAS  Google Scholar 

  • Cross, C. E., Oxygen radicals and human disease.Ann. Intern. Med., 107, 526–545 (1987).

    PubMed  CAS  Google Scholar 

  • Ito, H., Onoue, S., and Yoshida, T., Isoflavonoids fromBelamcanda chinensis.Chem. Pharm. Bull., 49, 1229–1231 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Jung, S. H., Lee, Y. S., Lee, S., Lim, S. S., Kim, Y. S., Ohuchi, K., and Shin, K. H., Anti- angiogenic and anti-tumor activities of isoflavonoids from the rhizomes ofBelamcanda chinensis.Planta Med., 69, 617–622 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Kim, Y. P., Yamada, M., Lim, S. S., Lee, S. H., Ryu, N., Shin, K. H., and Ohuchi, K., Inhibition by tectorigenin and tectoridin of prostaglandin E2 production and cyclooxygenase-2 induction in rat peritoneal macrophages.Biochim. Biophys. Acta, 10, 399–407 (1999).

    CAS  Google Scholar 

  • Kovacic, P. and Jacintho, J. D., Mechanisms of carcinogenesis: focus on oxidative stress and electron transfer.Curr. Med. Chem., 8, 773–796 (2001).

    PubMed  CAS  Google Scholar 

  • Kumaravelu, P., Dakshinamoorthy, D. P., Subramaniam, S., Devaraj, H., and Devaraj, N. S., Effect of eugenol on drugmetabolizing enzymes of carbon tetrachloride-intoxicated rat liver.Biochem. Pharmacol., 49, 1703–1707 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Kubow, S., Toxicity of dietary lipid peroxidation products.Trends Food Sci. Tech., 1, 67–71 (1990).

    Article  CAS  Google Scholar 

  • Lee, K. T., Sohn, I. C., Kim, D. H., Choi, J. W., Kwon, S. H., and Park, H. J., Hypoglycemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activityin vitro.Arch. Pharm. Res., 23, 461–466 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Lowry, O. H., Posenbrough, N. N., Farr, A. L., and Randall, R. J., Protein measurement with the folin phenol reagent.J. Biol. Chem., 193, 265–275 (1951).

    PubMed  CAS  Google Scholar 

  • McCord, J. M. and Fridovich, I., Superoxide dismutase: An enzymatic function for erythrocuprein (hemocoprein).J. Biol. Chem., 244, 6049–6055 (1969).

    PubMed  CAS  Google Scholar 

  • Park, K. Y., Jung, G. O., Choi, J. W., Lee, K. T., and Park, H. J., Potent antimutagenic and their anti-lipid peroxidative effect of kaikasaponin III and tectorigenin from the flower ofPueraria thunbergiana.Arch. Pharm. Res., 25, 320–324 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Pike, J. and Chandra, R. K., Effect of vitamin and trace element supplementation on immune indices in healthy elderly.Int. J. Vitamin Nutr. Res., 65, 117–120 (1995).

    CAS  Google Scholar 

  • Recknagel, R. O., Glende Jr, E. A., Dolak, J. A., and Waller, R. L., Mechanisms of carbon tetrachloride toxicity.Pharmacol. Therapeut., 43, 139–154 (1989).

    Article  CAS  Google Scholar 

  • Reitman, S. and Frankel, S., A chlorotrimetric method for the determination of sGOT and sGPT.Am. J. Clin. Pathol., 28, 56–63 (1957).

    PubMed  CAS  Google Scholar 

  • Rigo, A. and Rotilio, G., Simultaneous determination of superoxide dismutase and catalase in biological materials by polarography.Anal. Biochem., 81, 157–166 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Wei, H., Bowen, R., Cai, Q., Barnes, S., and Wang, Y., Antioxidant and antipromotional effects of the soybean isoflavone genistein.Proc. Soc. Exp. Biol. Med., 208, 124–130 (1995).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Natural Products Research Institute, College of Pharmacy, Seoul National University, 110-460, Seoul, Korea

    Sang Hoon Jung, Sanghyun Lee, Kuk Hyun Shin & Yeong Shik Kim

  2. Natural Products Research Center, Korea Institute of Science and Technology, 136-791, Seoul, Korea

    Sang Hoon Jung

  3. Seokwon Life Science Research Institute, 413-832, Paju, Korea

    Yeon Sil Lee & Kuk Hyun Shin

  4. Silver Biotechnology Research Center, Hallym University, 200-702, Chunchon, Korea

    Soon Sung Lim

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  1. Sang Hoon Jung
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  2. Yeon Sil Lee
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  3. Soon Sung Lim
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Correspondence to Yeong Shik Kim.

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Jung, S.H., Lee, Y.S., Lim, S.S. et al. Antioxidant activities of isoflavones from the rhizomes ofbelamcanda chinensis on carbon tetrachloride-lnduced hepatic injury in rats. Arch Pharm Res 27, 184–188 (2004). https://doi.org/10.1007/BF02980104

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  • DOI: https://doi.org/10.1007/BF02980104

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