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Detection of resistance to sterol demethylation-inhibiting (DMI) fungicides inCercospora beticola and efficacy of control of resistant and sensitive strains with flutriafol

  • Phytopathology/Mycology
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Abstract

Single-lesion isolates ofCercospora beticola (n=150) were collected in 1998 from sugar beet fields in the area of Serres, N. Greece. In this area, sterol demethylation-inhibiting (DMI) fungicides have been used for almost 20 years to control sugar beet leaf spot. The sensitivity of these isolates to the DMI fungicides flutriafol and difenoconazole (EC50 values) was determined on the basis of inhibition of mycelial growth at several fungicide concentrations. The relative growth (RG) of isolates was correlated at all tested concentrations with the respective EC50 values, indicating that RG provides a reliable estimate for the sensitivity of the isolates. The highest correlation coefficients were obtained for concentrations of 1 µg ml−1 flutriafol and of 0.05 µg ml−1 difenoconazole, respectively. Consequently, they are proposed for monitoring of DMI sensitivity inC. beticola populations, as single discriminatory concentrations in a simplified test method. Based on the RG values at the discriminatory concentration of 1 µg ml−1 flutriafol,C. beticola isolates were classified as either resistant or sensitive. The efficacy of flutriafol, applied at the commercially recommended dose, in controlling Cercospora leaf spot was examined in field experiments conducted during 1999 and 2000. Disease incidence in plots artificially inoculated with resistant isolates and treated with flutriafol was significantly higher than in similar plots inoculated with sensitive strains. These results suggest that poor disease control after application of flutriafol may be based on the presence of resistant strains within the pathogen population in northern Greece. This emphasizes the risk of the development of practical resistance if there is increased frequency of such strains within the population.

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References

  1. Anon. (1988) Fungicide resistance: definitions and use of terms.EPPO Bull. 18:569–574.

    Article  Google Scholar 

  2. Bleiholder, H. and Weltzien, H.C. (1972) Contributions to the epidemiology ofCercospora beticola on sugar beet. III. Geopathological studies.Phytopathol. Z. 73:46–68.

    Article  Google Scholar 

  3. Brent, K.J. (1992) Monitoring fungicide resistance — purposes, procedures and progress.in: Devonshire, A.L. and Hollomon, D.W. [Eds.] Resistance ’91: Achievements and Developments in Combating Pesticide Resistance. Ellis Science Publishers, London, UK. pp. 1–18.

    Google Scholar 

  4. Erickson, E.O. and Wilcox, W.F. (1997) Distributions of sensitivities to three sterol demethylation inhibitor fungicides among populations ofUncinula necator sensitive and resistant to triadimefon.Phytopathology 87:784–791.

    Article  PubMed  CAS  Google Scholar 

  5. Georgopoulos, S.G. (1987) The development of fungicide resistance.in: Wolfe, M.S. and Caten, C.E. [Eds.] Populations of Plant Pathogens: Their Dynamics and Genetics. Blackwell Scientific Publications, Oxford, UK. pp. 239–251.

    Google Scholar 

  6. Horsfall, J.G. and Barrat, R.W. (1945) An improved rating system for measuring plant diseases.Phytopathology 35:655.

    Google Scholar 

  7. Ioannidis, P.M. (1994) Fungicides, chemicals and techniques for controllingCercospora beticola Sacc. in Greece.Proc. Mediterranean Committee Meeting of Int. Inst. Beet Research (I.I.R.B.) (Thessaloniki, Greece), pp. 139–151.

  8. Karaoglanidis, G.S., Ioannidis, P.M. and Thanassoulopoulos, C.C. (2000) Reduced sensitivity ofCercospora beticola isolates to sterol demethylation inhibiting fungicides.Plant Pathol. 49:567–572.

    Article  CAS  Google Scholar 

  9. Karaoglanidis, G.S., Ioannidis, P.M. and Thanassoulopoulos, C.C. (2002) Changes in sensitivity ofCercospora beticola populations to sterol-demethylation-inhibiting fungicides during a 4-year period in northern Greece.Plant Pathol. 51:55–62.

    CAS  Google Scholar 

  10. Köller, W. (1991) Fungicide resistance in plant pathogens.in: Pimentel, D. and Hanson, A.A. [Eds.] CRC Handbook of Pest Management in Agriculture. CRC Press, Boca Raton, FL, USA. pp. 679–720.

    Google Scholar 

  11. Köller, W., Parker, D.M. and Reynolds, K.L. (1991) Baseline sensitivities ofVenturia inaequalis to sterol demethylation inhibitors.Plant Dis. 75:726–728.

    Google Scholar 

  12. Köller, W., Wilcox, W.F., Barnard, J., Jones, A.L. and Brown, P.G. (1997) Detection and quantification of resistance ofVenturia inaequalis populations to sterol demethylation inhibitors.Phytopathology 87:184–190.

    Article  PubMed  Google Scholar 

  13. Peever, T.B. and Milgroom, M.G. (1992) Inheritance of triadimenol resistance inPyrenophora teres.Phytopathology 82:821–828.

    Article  CAS  Google Scholar 

  14. Romero, R.A. and Sutton, T.B. (1997) Sensitivity ofMycosphaerella fijiensis, causal agent of Black Sigatoka of banana, to propiconazole.Phytopathology 87:96–100.

    Article  PubMed  CAS  Google Scholar 

  15. Scheinpflug, H. and Kuck, K.H. (1987) Sterol biosynthesis inhibiting piperazine, pyridine, pyrimidine and azole fungicides — properties, applications and mechanisms of action.in: Lyr, H. [Ed.] Modern Selective Fungicides — Properties, Applications and Mechanisms of Action. VEB Gustav Fischer Verlag, Jena, and Longman Group, UK Ltd, London, UK. pp. 173–204.

    Google Scholar 

  16. Shane, W.W. and Teng, P.S. (1992) Impact of Cercospora leaf spot on root weight, sugar yield and purity ofBeta vulgaris.Plant Dis. 76:812–820.

    CAS  Google Scholar 

  17. Skylakakis, G. and Hollomon, D.W. (1987) Epidemiology of fungicide resistance.in: Ford, M.G., Hollomon, D.W., Khambay, B.P.S. and Sawicki, R.M. [Eds.] Combating resistance to Xenobiotics — Biological and Chemical Approaches. Ellis Horwood Ltd., Chichester, UK. pp. 94–103.

    Google Scholar 

  18. Smith, F.D., Parker, D.M. and Köller, W. (1991) Sensitivity distribution ofVenturia inaequalis to the sterol demethylation inhibitor flusilazole: Baseline sensitivity and implications for resistance monitoring.Phytopathology 81:392–396.

    Article  CAS  Google Scholar 

  19. Stahle-Csech, U. and Gisi, U. (1991) Determination of the sensitivity to DMI fungicides ofCercospora beticola on sugar beet.EPPO Bull. 21:321–323.

    Article  Google Scholar 

  20. Tuite, J. (1969) Plant Pathological Methods. Burgess Publishing Co., Minneapolis, MN, USA.

    Google Scholar 

  21. Wolf, P.F.J. and Verreet, J.A. (2002) The IPM sugar beet model — An integrated pest management system in Germany for the control of fungal leaf diseases in sugar beet.Plant Dis. 86:336–344.

    Article  Google Scholar 

  22. Wolfe, M.S. (1982) Dynamics of the pathogen population in relation to fungicide resistance.in: Dekker, J. and Georgopoulos, S.G. [Eds.] Fungicide Resistance in Crop Protection. Center for Agricultural Publishing and Documentation, Wageningen, the Netherlands. pp. 139–148.

    Google Scholar 

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Authors and Affiliations

  1. Faculty of Agriculture, Plant Pathology Laboratory, Aristotelian University of Thessaloniki, 54006, Thessaloniki, Greece

    G. S. Karaoglanidis

  2. Plant Protection Dept., Hellenic Sugar Industry S.A., 51110, Larissa, Greece

    D. A. Karadimos

  3. Plant Protection Department, Hellenic Sugar Industry S.A., 59032, Plati Imathias, Greece

    P. M. Ioannidis

Authors
  1. G. S. Karaoglanidis
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  2. D. A. Karadimos
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  3. P. M. Ioannidis
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Correspondence to G. S. Karaoglanidis.

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http://www.phytoparasitica.org posting July 13, 2003.

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Karaoglanidis, G.S., Karadimos, D.A. & Ioannidis, P.M. Detection of resistance to sterol demethylation-inhibiting (DMI) fungicides inCercospora beticola and efficacy of control of resistant and sensitive strains with flutriafol. Phytoparasitica 31, 373–380 (2003). https://doi.org/10.1007/BF02979809

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  • DOI: https://doi.org/10.1007/BF02979809

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