Skip to main content
SpringerLink
Log in

Genetic diversity of the entomopathogenVerticillium lecanii on the basis of vegetative compatibility

  • Phytopathology/Mycology
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Forty-three isolates ofVerticillium lecanii from insects, phytopathogenic fungi and other substrates were tested for vegetative compatibility by observing heterokaryon formation among complementary nitrate-nonutilizing (nit) mutants.nit mutants were isolated from 42/43 strains examined. Twenty-one isolates were self-incompatible, and the remaining 21 isolates were divided into 14 vegetative compatibility groups (VCGs): ten containing only a single strain each, and the remaining four containing two to four isolates each. Members of isolates in each of these VCGs all shared the same IGS haplotype. Further, the isolates within a VCG were correlated with one another in part by fragment patterns of mt-LrDNA, -SrDNA, Bt-2 and H4 region, by PCR-RFLP and -SSCP, but not by dsRNA. Two isolates belonging to VL-J2 have high virulence to aphids, whereas strains from VL-J1 lack this character. These findings indicate that two VCGs (VL-J1 and -J2) may originate from two distinct clonal lineages. Alternatively, high VCG diversity and HSI frequency ofV. lecanii might be associated with an array of distinct lineages. These data not only suggest relationships among DNA polymorphisms, virulence, and VCG, but also demonstrate genetic heterogeneity ofV. lecanii.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allen, D.J. (1982)Verticillium lecanii on the bean rust fungus,Uromyces appendiculatus.Trans. Br. Mycol. Soc. 79:362–364.

    Google Scholar 

  2. Askary, H., Benhamou, N. and Brodeur, J. (1997) Ultrastructural and cytochemical investigation of the antagonistic effect ofVerticillium lecanii on cucumber powdery mildew.Phytopathology 87:359–368.

    Article  PubMed  CAS  Google Scholar 

  3. Clarkson, J.M. and Heale, J.B. (1985) Heterokaryon compatibility and genetic recombination within a host plant between hop wilt isolates ofVerticillium albo-atrum.Plant Pathol. 34:129–138.

    Article  Google Scholar 

  4. Correll, J.C., Gordon, T.R. and McCain, A.H. (1988) Vegetative compatibility and pathogenicity ofVerticillium albo-atrum.Phytopathology 78:1017–1021.

    Article  Google Scholar 

  5. Correll, J.C., Klittich, C.J.R. and Leslie, J.F. (1987) Nitrate nonutilizing mutants ofFusarium oxysporum and their use in vegetative compatibility tests.Phytopathology 77:1640–1646.

    Article  Google Scholar 

  6. Correll, J.C., Klittich, C.J.R. and Leslie, J.F. (1989) Heterokaryon self-incompatibility inGibberella fujikuroi (Fusarium moniliforme).Mycol. Res. 93:21–27.

    Google Scholar 

  7. Correll, J.C., Puhalla, J.E. and Schneider, R.W. (1986) Identification ofFusarium oxysporum f. sp.apii on the basis of colony size, virulence, and vegetative compatibility.Phytopathology 76:396–400.

    Article  Google Scholar 

  8. Couteaudier, Y. and Viaud, M. (1997) New insights into population structure ofBeauveria bassiana with regard to vegetative compatibility groups and telomeric restriction fragment length polymorphisms.FEMS Microbiol. Ecol. 22:175–182.

    Article  CAS  Google Scholar 

  9. Ebihara, Y., Nagao, H., Koike, M., Shiraishi, T. and Iijima, T. (1999) How do Japanese isolates ofVerticillium dahliae correspond with standardized VCG testers?Mycoscience 40:333–343.

    Article  Google Scholar 

  10. Hall, R.A. (1980) Laboratory infection of insects byVerticillium lecanii strains isolated from phytopathogenic fungi.Trans. Br. Mycol. Soc. 74:445–446.

    Google Scholar 

  11. Hall, R.A. (1981) The fungusVerticillium lecanii as a microbial insecticide against aphids and scales.in: Burges, H.D. [Ed.] Microbial Control of Pest and Plant Diseases. Academic Press, New York, NY. pp. 483–498.

    Google Scholar 

  12. Hall, R.A. (1984) Epizootic potential for aphids of different isolates of the fungusVerticillium lecanii.Entomophaga 29:311–321.

    Article  Google Scholar 

  13. Hisao, W.F., Bidochka, M.J. and Khachatourians, G.G. (1992) Effect of temperature and relative humidity on the virulence of the entomopathogenic fungus,Verticillium lecanii, toward the oat-bird berry aphid,Rhopalosiphum padi (Hom., Aphidiae).J. Appl. Entomol. 114:484–490.

    Google Scholar 

  14. Jackson, C.W. and Heale, J.B. (1987) Parasexual crosses by hyphal anastomosis and protoplast fusion in the entomopathogenVerticillium lecanii.J. Gen. Microbiol. 133:3537–3547.

    Google Scholar 

  15. Joaquim, T.R. and Rowe, R.C. (1990) Reassessment of vegetative compatibility relationships among strains ofVerticillium dahliae using nitrate-nonutilizing mutants.Phytopathology 80:1160–1166.

    Article  Google Scholar 

  16. Jun, Y., Bridge, P.D. and Evans, H.C. (1991) An integrated approach to the taxonomy of the genusVerticillium.J. Gen. Microbiol. 137:1437–1444.

    Google Scholar 

  17. Katan, T., Zamir, D., Sarfatti, M. and Katan, J. (1991) Vegetative compatibility groups and subgroups inFusarium oxysporum f.sp.radicis-lycopersici.Phytopathology 81:255–262.

    Article  CAS  Google Scholar 

  18. Korolev, N. and Gindin, G. (1999) Vegetative compatibility in the entomopathogenVerticillium lecanii.Mycol. Res. 103:833–840.

    Article  Google Scholar 

  19. Korolev, N., Katan, J. and Katan, T. (2000) Vegetative compatibility groups ofVerticillium dahliae in Israel: Their distribution and association with pathogenicity.Phytopathology 90:529–536.

    Article  PubMed  CAS  Google Scholar 

  20. Korolev, N. and Katan, T. (1997) Improved medium for selecting nitrate-nonutilizing (nit) mutants ofVerticillium dahliae.Phytopathology 87:1067–1070.

    Article  PubMed  CAS  Google Scholar 

  21. Korolev, N. and Katan, T. (1999) Vegetative compatibility grouping inVerticillium nigrescens andV. tricorpus.Mycol. Res. 103:65–76.

    Article  Google Scholar 

  22. Kuter, G.A. (1984) Hyphal interactions betweenRhizoctonia solani and someVerticillium species.Mycologia 76:936–940.

    Article  Google Scholar 

  23. Leslie, J.F. (1993) Fungal vegetative compatibility.Annu. Rev. Phytopathol. 31:127–150.

    Article  PubMed  CAS  Google Scholar 

  24. Mor, H., Gindin, G., Ben-Ze’ev, I.S., Raccah, B., Geschtovt, N.U., Ajtkhozhina, N.et al. (1996) Diversity among isolates ofVerticillium lecanii as expressed by DNA polymorphism and virulence towardsBemisia tabaci.Phytoparasitica 24:111–118.

    Google Scholar 

  25. Puhalla, J.E. (1985) Classification of strains ofFusarium oxysporum on the basis of vegetative compatibility.Can. J. Bot. 63:179–183.

    Article  Google Scholar 

  26. Puhalla, J.E. and Hummel, M. (1983) Vegetative compatibility groups withinVerticillium dahliae.Phytopathology 73:1305–1308.

    Article  Google Scholar 

  27. Raghavendra-Rao, N.N. and Pavgi, M.S. (1977) Two mycoparasites on powdery mildews.Sydowia 30:145–147.

    Google Scholar 

  28. Roberts, D.M., Bainbridge, B.W., Evans, H.C. and Heale, J.B. (1995) Genome analysis in isolates ofVerticillium, V. albo-atrum, V. dahliae andV. lecanii.Phytoparasitica 23:40 (abstr.).

    Google Scholar 

  29. Spencer, D.M. and Atkey, P.T. (1981) Parasitic effects ofVerticillium lecanii on two rust fungi.Trans. Br. Mycol. Soc. 77:535–542.

    Google Scholar 

  30. St. Leger, R.J., Joshi, L. and Roberts, D.W. (1997) Adaptation of proteases and carbohydrases of saprophytic, phytopathogenic and entomopathogenic fungi to the requirements of their ecological niches.Microbiology 143:1983–1992.

    Article  PubMed  CAS  Google Scholar 

  31. Strausbaugh, C.A., Schroth, M.N., Weinhold, A.R. and Hancock, J.G. (1992) Assessment of vegetative compatibility ofVerticillium dahliae tester strains and isolates from California potatoes.Phytopathology 82:61–68.

    Article  Google Scholar 

  32. Sugimoto, M., Koike, M., Hiyama, N. and Nagao, H. (2003) Genetic, morphological, and virulence characterization of the entomopathogenic fungusVerticillium lecanii.J. Invertebr. Pathol. 82:176–187.

    Article  PubMed  CAS  Google Scholar 

  33. Sugimoto, M., Koike, M. and Nagao, H. (2001) Ribotyping of entomopathogenicVerticillium lecanii in Japan.Phytoparasitica 29:413–420.

    CAS  Google Scholar 

  34. Sugimoto, M., Koike, M., Okumura, K., Nagao, H., Ebihara, Y. and Sumino, A. (2002) Genetic diversity ofVerticillium albo-atrum in Japan. Biodiversity in Plant Pathology.Proc. 5th Congress of the European Foundation for Plant Pathology (Taormina-Giardini Naxos, Italy), pp. 161–166.

  35. Typas, M.A., Mavridou, A. and Kouvelis, V.N. (1998) Mitochondrial DNA differences provide maximum intraspecific polymorphism in the entomopathogenic fungiVerticillium lecanii andMetarhizium anisopliae, and allow isolate detection / identification.in: Bridge, P., Couteaudier, Y. and Clarkson, J. [Eds.] Molecular Variability of Fungal Pathogens. CAB International, Wallingford, UK. pp. 227–237.

    Google Scholar 

  36. Wakatabe, D., Nagao, H., Arai, H., Shiraishi, T., Koike, M. and Iijima, T. (1997) Vegetative compatibility groups of Japanese isolates ofVerticillium dahliae.Mycoscience 38:17–23.

    Article  Google Scholar 

  37. Yokomi, R.K. and Gottwald, T.R. (1988) Virulence ofVerticillium lecanii isolates in aphids determined by detached-leaf bioassay.J. Invertebr. Pathol. 51:250–258.

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Midori Sugimoto

    Present address: Okinawa Prefectural Agricultural Experiment Station, 903-0814, Naha, Japan

Authors and Affiliations

  1. Dept. of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080-8555, Hokkaido, Japan

    Midori Sugimoto, Masanori Koike, Kayo Okumura, Masayuki Tani & Katsuhisa Kuramochi

  2. Institute of Agriculture and Forestry, University of Tsukuba, 305-8572, Tsukuba, Japan

    Hideyuki Nagao

Authors
  1. Midori Sugimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masanori Koike
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideyuki Nagao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kayo Okumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masayuki Tani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Katsuhisa Kuramochi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masanori Koike.

Additional information

http://www.phytoparasitica.org posting Sept. 30, 2003.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sugimoto, M., Koike, M., Nagao, H. et al. Genetic diversity of the entomopathogenVerticillium lecanii on the basis of vegetative compatibility. Phytoparasitica 31, 450–457 (2003). https://doi.org/10.1007/BF02979738

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02979738

Key words

Search

Navigation