Skip to main content

Stability of antineoplaston A10 in aqueous solution


The analysis method and stability test of antineoplaston A10, a new anticancer drug candidate, were established. A10 and phenylacetyl- L-glutamine, one of the degradation products, can be detected by high-performance liquid chromatography. The degradation kinetics of antineoplaston A10 in aqueous solutions from pH 1 to 10 buffers were carried out at 40, 50 and 60°C. Pseudo-first order kinetics were obtained throughout the entire pH ranges studied. The pH-rate profiles showed that antineoplaston A10 was very unstable in alkaline conditions and most stable at pH4.

This is a preview of subscription content, access via your institution.

References cited

  • Burzynski, S. R., Synthetic antineoplastons and analogs, Drugs of the Future, 11, 679–688 (1986).

    Google Scholar 

  • Burzynski, S. R., Hendry, L. B., Mohabbat, M. O., Liau, M. C., Khalid, M. and Burzynski, B., Purification, structure determination, synthesis and animal toxicity studies of antineoplaston A10, Proc. 13th Internat. Congr. Chemother., Vienna, 12, 11–14 (1983).

    Google Scholar 

  • Burzynski, S. R., Mohabbat, M. O. and Burzynski, B., Toxicology studies of oral formulation of antineoplaston A10 in cancer patients, Drugs. Exp. Clin. Res., 10, 611–619 (1984).

    Google Scholar 

  • Connors, K. A., Amidon, G. L. and Stella, V. J., Chemical Stability of Pharmaceuticals, 2nd Ed., John Wiley & Sons., New York, 1986, pp 8–31.

    Google Scholar 

  • Michalska, D., Theoretical investigations on the structure and potential binding sites of antineoplaston A 10 and experimental findings. Drugs Exptl. Clin. Res., 16, 342–349 (1990).

    Google Scholar 

  • Perrin, D. D. and Dempsey, B., Buffers for pH and metal ion control, Chapman and Hall, London, 1974, pp 40–50.

    Google Scholar 

  • Seo, H. K., Synthesis of antineoplaston A10 analogs and their antitumor activity, M. S. Thesis, Chonnam National University, 1994.

  • Taylor, R. and Shivji, A. S. H., A critical appraisal of drug stability testing methods, Pharm. Res., 4, 177–180 (1987).

    PubMed  Article  CAS  Google Scholar 

  • Wesolowski, J. W., Blaug, S. M., Chin, T. and Lach, J. L., Kinetics of degradation of glutethimide, J. Pharm. Sci., 57, 811–815 (1968).

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

Oh, I.J., Lee, J.M., Lee, Y.B. et al. Stability of antineoplaston A10 in aqueous solution. Arch. Pharm. Res. 18, 75–78 (1995).

Download citation

  • Received:

  • Issue Date:

  • DOI:

Key words

  • Antineoplaston A10
  • Stability
  • Hydrolysis
  • Anticancer agent