Abstract
A new high-performance liquid chromatographic method with column switching has been developed for the determination of omeprazole in plasma. The plasma samples were injected onto a Bondapak phenyl/corasil (37–50 μm) precolumn and polar plasma components were washed with 0.06 M borate buffer. After valve switching, the concentrated drug were eluted in the back-flush mode and separated on a μ-Bondapak C18 column with acetonitrile-phosphate buffer as the mobile phase. The method showed excellent precision, accuracy and speed with detection limit of 0.01 μg/ml−1. Total analysis time per sample was less than 20 min and the coefficients of variation for intra and inter-assay were less than 5.63%. This method has been successfully applied to plasma samples from rats after oral administration of omeprazole.
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Shim, SH., Bok, SJ. & Kwon, KI. Determination of omeprazole in rat plasma by HPLC with column switching. Arch. Pharm. Res. 17, 458–461 (1994). https://doi.org/10.1007/BF02979125
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DOI: https://doi.org/10.1007/BF02979125