Abstract
The water soluble long-chain crosslinker, sulfo-succinimidyl-6-[3′-(2-pyridyldithio)-propionamido]hexanoate (S-LC-SPDP) was used to prepare ricin A chain (RTA) immunotoxins constructed with whole IgG and Fab fragments of the anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody. In this study, a) S-LC-SPDP modification efficiencies of whole IgG and Fab, b) conjugation yields of the immunotoxins prepared and c)in vitro immunoreactivity and cytotoxicity of immunotoxins constructed were examined. IgG-RTA and Fab-RTA immunotoxins were prepared with 67.3% and 57.0% conjugation yields, respectively. These long spacer intermolecular linked immunotoxins were selectively immunorective and cytotoxic against to immunogenic Daudi cells but little or no-binding and cytotoxic against to antigen K562 cells. Both IgG-RTA and Fab-RTA immunotoxins were 210- and 45-fold more active than intact RTAin vitro, respectively.
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Woo, B.H., Lee, J.T. & Lee, K.C. Ricin A immunotoxins of IgG and Fab of anti-CALLA monoclonal antibody: Effect of water soluble long-chain SPDP on conjugate yield, immunoselectivity and cytotoxicity. Arch. Pharm. Res. 17, 452–457 (1994). https://doi.org/10.1007/BF02979124
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DOI: https://doi.org/10.1007/BF02979124