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Angiotensin II-mediated stimulation of phospholipase D in rabbit kidney proximal tubule cells

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Abstract

The present study was undertaken to demonstrate whether or not angiotensin II activates a phospholipase D in rabbit kidney proximal tubule cells. By measuring the formation of [3H]phosphatidic acid and [3H]phosphatidylethanol, we elucidate the direct stimulation of phospholipase D by angiotensin II. Angiotensin II leads to a rapid increase in [3H]phosphatidic acid and [3H]diacylglycerol, and [3H]phosphatidic acid formation preceeded the formation of [3H]diacylglycerol. This result suggests that some phosphatidic acid seems to be formed directly from phosphatidylcholine by the action of phospholipase D, not from the action of diacylglycerol kinase on the diacylglycerol. In addition, the other mechanisms by which phospholipase D is activated was examined. We have found that phospholipase D was activated by extracellular calcium ion. It has also been shown that angiotensin II may activate phospholipase D through protein kinase C-independent pathway.

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Authors and Affiliations

  1. College of Pharmacy, Kyung Hee University, 130-701, Seoul, Korea

    Jin Ho Jung & Sung Hyun Chung

  2. College of Medicine, Kyung Hee University, 130-701, Seoul, Korea

    Jee Chang Jung

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  1. Jin Ho Jung
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Jung, J.H., Jung, J.C. & Chung, S.H. Angiotensin II-mediated stimulation of phospholipase D in rabbit kidney proximal tubule cells. Arch. Pharm. Res. 17, 405–410 (1994). https://doi.org/10.1007/BF02979115

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  • DOI: https://doi.org/10.1007/BF02979115

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