Archives of Pharmacal Research

, Volume 28, Issue 9, pp 1079–1085 | Cite as

Chitosan Oligosaccharide inhibits203HgCl2-induced genotoxicity in mice: Micronuclei occurrence and chromosomal aberration

  • Hyun Joong Yoon
  • Haeng Soon Park
  • Hee-Seung Bom
  • Young Bok Roh
  • Jong Se Kim
  • Young Ho KimEmail author
Articles Drug Development


The purpose of this study was to investigate the safety of chitosan oligosaccharide and the effects of chitosan oligosaccharide on mercury induced genotoxicity in mice using the micronuclei and chromosome aberration. The micronuclei test was performed by microscopic examination (×1,000, stained using a May-Grunwald solution) after administering 0.01, 0.1, and 1% (10 mg/mL) chitosan oligosaccharide for 7, 60, and 180 daysad libitum in mice. Total micronuclei of 1,000 polychromatic erythrocytes were recorded for each group. There was no difference between the untreated and experimental groups. The intake periods and concentrations of chitosan oligosaccharide did not affect the occurrence of micronuclei in bone marrow cells (P>0.05). The chromosomal aberration test was performed by microscopic examination (×1,000, stained using a 4% Giemsa solution) after administering the same concentration of chitosan oligosaccharide to mice, in F1, F2, F3 generations and parents. The frequency of chromosomal aberrations was defined as [Ydr=(D+R)/total number of counted lymphocytes]. Similar to the micronuclei test, there was no difference between the untreated and treated groups. These results showed that the intake periods and concentrations of chitosan oligosaccharide did not affect chromosomal aberrations in bone marrow cells (P>0.05). To investigate the effect of chitosan oligosaccharide on mercury-induced chromosome aberration, mice in each condition were supplied with203HgCl2 and chitosan oligosaccharidead libitum. Chitosan oligosaccharide significantly inhibited203HgCl2-induced chromosome aberration in mice. Based on the results of this study, it may be concluded that the chitosan oligosaccharide is a nontoxic material that could be used as a suppressor of heavy metal-induced genotoxicity.

Key words

Chitosan oligosaccharide Mercury Micronucleus Chromosomal aberration 


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Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Hyun Joong Yoon
    • 3
    • 4
  • Haeng Soon Park
    • 3
    • 4
  • Hee-Seung Bom
    • 1
  • Young Bok Roh
    • 2
  • Jong Se Kim
    • 2
  • Young Ho Kim
    • 3
    • 4
  1. 1.Department of Nuclear MedicineChonnam University HospitalGwangjuKorea
  2. 2.Department of Biology, College of Natural ScienceChosun UniversityGwangjuKorea
  3. 3.College of PharmacyChonnam National UniversityGwangjuKorea
  4. 4.Research Institute of Drug DevelopmentChonnam National UniversityGwangjuKorea

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