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Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis

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Abstract

The EtOAc-soluble fraction from a methanolic extract ofHovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1–8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds6 and8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound6 had a DPPH free radical scavenging effect with an IC50 value of 57.7 μM, and a superoxide anion radical scavenging effect with an 8.0 μM. Both compounds6 and8 had ABTS cation radical scavenging effects with IC50 values of 7.8 μM and 23.7 μM, respectively. These results suggest that compounds6 and8 could be neuroprotectants owing to their free radical scavenging activities.

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Authors and Affiliations

  1. Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, Yuseong, P.O. Box 115, 305-333, Daejeon, Korea

    Gao Li, Byung-Sun Min, Changji Zheng, Joongku Lee, Sei-Ryang Oh, Kyung-Seop Ahn & Hyeong-Kyu Lee

  2. College of Pharmacy, Yanbian University, 133000, Yanji, P. R. China

    Gao Li & Changji Zheng

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  1. Gao Li
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  2. Byung-Sun Min
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  3. Changji Zheng
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  4. Joongku Lee
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  5. Sei-Ryang Oh
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  6. Kyung-Seop Ahn
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  7. Hyeong-Kyu Lee
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Correspondence to Hyeong-Kyu Lee.

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Li, G., Min, BS., Zheng, C. et al. Neuroprotective and free radical scavenging activities of phenolic compounds fromHovenia dulcis . Arch Pharm Res 28, 804–809 (2005). https://doi.org/10.1007/BF02977346

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  • DOI: https://doi.org/10.1007/BF02977346

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