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In vitro antioxidant activity of some selectedprunus species in Korea

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Abstract

In the course of the investigations of natural antioxidants, we examined the antioxidant activities of the methanol (MeOH) extracts of some selectedPrunus species, includingP. buergeriana, P. davidiana, P. padus, P. pendula for.ascendens, P. sargentii, P. serrulata var. spontanea andP. yedoensis by three methods as represented by the 1,1-diphenyl-2-picrylhy-drazyl (DPPH) radical, total ROS (reactive oxygen species) and the peroxynitrite (ONOO-) scavenging activity tests. We also evaluated the activities of the organic solvent-soluble fractions, including the dichloromethane (CH2CI2), ethyl acetate (EtOAc),n-butanol (n-BuOH) fractions and the water (H2O) layer ofP. serrulata var.spontanea leaves. By means of bioassay-directed fractionation, we isolated eleven known flavonoids (1–11) from the EtOAc soluble fraction of the MeOH extract of thePrunus serrulata var.spontanea leaves, exhibiting strong antioxidant activity and characterized as prunetin (1), genistein (2), quercetin (3), prunetin 4′-O-β-glucopyranoside (4), kaempferol 3-O-β-arabinofuranoside (5), prunetin 5-O-β-glucopyranoside (6), kaempferol 3-O-β-xylopyranoside (7), genistin (8), kaempferol 3-O-β-glucopyranoside (9), quercetin 3-O-β-glucopyranoside (10) and kaempferol 3-O-β-xylopyranosyl-(1→2)-β-glucopyr-anoside (11). Compounds3 and10 showed good activities in all tested model systems. Compounds2 and8 showed scavenging activities in the DPPH and ONOCr tests, while compounds5,7,9 and11 were active in the ONOO- and ROS tests. On the other hand, compounds1,4 and6 did not show any activities in the tested model systems.

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Authors and Affiliations

  1. Faculty of Food Science and Biotechnology, Pukyong National University, 608-737, Pusan, Korea

    Hyun Ah Jung & Jae Sue Choi

  2. College of Pharmacy, Pusan National University, 609-735, Pusan, Korea

    Ae Ra Kim & Hae Young Chung

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  1. Hyun Ah Jung
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Correspondence to Jae Sue Choi.

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Jung, H.A., Kim, A.R., Chung, H.Y. et al. In vitro antioxidant activity of some selectedprunus species in Korea. Arch Pharm Res 25, 865–872 (2002). https://doi.org/10.1007/BF02977006

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