Skip to main content
SpringerLink
Log in

Detection and characterization of 45 kDa platelet activating factor acetylhydrolase in cerebrospinal fluid of children with meningitis

  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

Platelet activating factor acetylhydrolase (PAF-AH) activity has been identified in cerebrospinal fluid (CSF) samples taken from children with meningitis. We reported that PAF-AH activity is significantly increased, by about 3 fold, in patients with meningitis compared to control subjects. Because of limited knowledge about this enzyme in CSF, we examined the biochemical properties of CSF PAF-AH. PAF-AH of CSF was calcium independent, showed a broad pH spectrum and was relatively heat stable. In addition, this enzyme activity was strongly inhibited by phenylmethanesulfonyl fluoride (PMSF), partially inhibited byp-bromophenacylbromide (p-BPB), uninhibited by iodoacetamide, and moderately stimulated by dithiothreitol (DTT). PAF-AH of CSF did not degrade phospholipid with a long chain fatty acyl group atsn-2 position. This enzyme hydrolyzed PAF and oxidatively modified phosphatidylcholine. Furthermore, we identified a monomeric polypeptide with a molecular weight of approximately 45 kDa by Western blot using human plasma PAF-AH antibody. These results suggested that plasma type PAF-AH activity exist in CSF taken from children with meningitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arai, H., Inoue, K., Natori, Y., Banno, Y., Nozawa, Y., and Nojima, S., Intracellular phospholipase activities of Tetrahymena pyriformis.J. Biochem. (Tokyo), 97, 1525–1532 (1985).

    PubMed  CAS  Google Scholar 

  • Arditi, M., Manogue, K. R., Caplan, M., and Yogev, R., Cerebrospinal fluid cachectin/tumor necrosis factor-alpha and platelet-activating factor concentrations and severity of bacterial meningitis in children.J. Infect. Dis., 162, 139–147 (1990).

    PubMed  CAS  Google Scholar 

  • Bazan, N. G., Increased levels of brain free acids after electroconvulsive shock.Biochim. Biophys. Acta., 218, 1–10 (1970).

    PubMed  CAS  Google Scholar 

  • Blank, M. L., M. N. Hall, E. A. Grees, and F. Snyder., Inactivation of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine by a plasma acetylhydrolase; higher activities in hypertensive rats.Biochem. Biophys. Res. Commun., 113, 666–671 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Chang, H. W., Kwon, S. H., Kim, H. M., Lee, K. S., Kim, M. S., Moon, T. C., and Baek, S. H., Platelet-activating factor acetylhydrolase activity in cerebrospinal fluid of children with acute systemic or neurological illness.Ann. Neurol., 51(6), 760–763 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Chien, K. R., Abrams, J., Serroni, A., Martin, J. T., and Farber, J. L., Accelerated phospholipid degradation and associated membrane dysfunction irreversible, ischemic liver cell injury.J. Biol. Chem., 253, 4809–4817 (1978).

    PubMed  CAS  Google Scholar 

  • Edgar, A. D., Strosznajder, J., and Horrocks, L. A., Activation of ethanolamine phospholipase A2 in brain during ischemia.J. Neurochem., 39, 1111–1116 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Glende, E. A. Jr. and Pushpenden, C. K., Significance of glycogen mobilization and phospholipase A2 activation in carbon tetrachloride liver cell injury.Biochem. Pharmacol., 35, 3301–3307 (1986).

    Article  PubMed  CAS  Google Scholar 

  • Hattori, M., Arai, H., and Inoue, K., Purification and characterization of bovine brain platelet-activating factor acetylhydrolase.J. Biol. Chem., 268, 18748–18753 (1993).

    PubMed  CAS  Google Scholar 

  • Hirashima, Y., Endo, S., Ohmori, T., Kato, R., and Takaku, A., Platelet-activating factor (PAF) concentration and PAF acetylhydrolase activity in cerebrospinal fluid of patients with subarachnoid hemorrhage.J. Neurosurg., 80, 31–36 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Ho, Y. S., Swenson, L., Derewenda, U., Serre, L., Wei, Y., Dauter, Z., Hattori, M., Adachi, T., Aoki, J., Arai, H., Inoue, K., and Derewenda, Z. S., Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer.Nature, 385, 89–93 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Itabe, H., Kobayashi, T., and Inoue, K., Generation of toxic phospholipid(s) during oxyhemoglobin-induced peroxidation of phosphatidylcholines.Biochim. Biophys. Acta, 961, 13–21 (1988).

    PubMed  CAS  Google Scholar 

  • Karasawa, K., Masayuki, Y., Morio, S., and Shoshichi, N., Purification and Characterization of Platelet-Activating Factor Acetylhydrolase from Peritoneal Fluid Obtained from Guinea Pigs after Endotoxin Shock.J. Biochem., 116, 374–379 (1994).

    PubMed  CAS  Google Scholar 

  • Matsuzawa, A., Hattori, K., Aoki, J., Arai, H., and Inoue, K., Protection against oxidative stress-induced cell death by intracellular platelet-activating factor-acetylhydrolase II.J. Biol. Chem., 272, 32315–32320 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Mitsuharu, H., Hiroyuki, A., and Keizo, I., Purification and characterization of bovine brain platelet-activating factor acetylhydrolase.J. Biol. Chem., 268, 18748–18753 (1993).

    Google Scholar 

  • Morgan, E. N., Boyle, E. M. Jr., Yun, W., Kovacich, J. C., Canty, T. G. Jr., Chi, E., Pohlman, T. H., and Verrier, E. D., Platelet-activating factor acetylhydrolase prevents myocardial ischemia-reperfusion injury.Circulation, 100, 11365–11368 (1999).

    Google Scholar 

  • Narahara, H., Tanaka, Y., Kawano, Y., Miyakawa, I., and Johnston, J. M., Platelet-activating factor acetylhydrolase activity in human follicular fluid.Adv. Exp. Med. Bio., 416, 121–127 (1996).

    CAS  Google Scholar 

  • Shimojo, T., Abe, M., and Ohta, M., Method the determination of saturated phosphatidylcholine.J. Lipd. Res., 15, 152–157 (1974).

    Google Scholar 

  • Son, S. Y., Kim, S. H., Baek, S. H., and Chang, H. W., Partial purification and characterization of PAF acetylhydrolase in human amniotic fluid.Arch. Pharm. Res., 20, 218–224 (1997).

    Article  CAS  PubMed  Google Scholar 

  • Stafforini, D. M., Prescott, S. M., and McIntyre, T. M., Human plasma platelet-activating factor acetylhydrolase: Association with lipoprotein particles and role in the degradation of platelet-activating factor.J. Biol. Chem., 262, 4223–4230 (1987).

    PubMed  CAS  Google Scholar 

  • Stafforini, D. M., Rollins, E. N., Prescott, S. M., and Mclntyre, T. M., The platelet-activating factor acetylhydrolase from human erythrocytes. Purification and properties.J. Biol. Chem., 268, 3857–3865 (1993).

    PubMed  CAS  Google Scholar 

  • Triggiani, M., De Marino, V., Sofia, M., Faraone, S., Ambrosio, G., Carratu, L., and Marone, G., Characterization of platelet-activating factor acetylhydrolase in human bronchoalveolar lavage.Am. J. Respir. Crit. Care. Med., 156, 94–100 (1997).

    PubMed  CAS  Google Scholar 

  • Tsuji, H., Furukawa, M., Ikeda, H., Asako, M., and Yamashita, T., The presence of platelet-activating factor acetylhydrolase in human middle ear effusions.ORL. J. Otorhinolaryngol. Relat. Spec., 60, 25–29 (1998).

    PubMed  CAS  Google Scholar 

  • Yanoshita, R., Kudo, I., Ikizawa, K., Chang, H. W., Kobayashi, S., Ohno, M., Nojima, S., and Inoue, K., Hydrolysis of platelet activating factor and its methylated analogs by acetylhydrolases.J. Biochem. (Tokyo), 103(5), 815–819 (1988).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Yeungnam University, 712-749, Gyongsan, Korea

    Tae Chul Moon, Mi Suk Kim, Su Jeong Lee, Tae Yoon Lee, Soon Hak Kwon, Suk Hwan Baek & Hyeun Wook Chang

  2. Department of Microbiology, College of Medicine, Yeungnam University, 705-717, Daegu, Korea

    Su Jeong Lee & Tae Yoon Lee

  3. Department of Pediatrics, Kyungpook National University Hospital, 700-721, Daegu, Korea

    Soon Hak Kwon

  4. Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, 705-717, Daegu, Korea

    Suk Hwan Baek

Authors
  1. Tae Chul Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mi Suk Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Su Jeong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tae Yoon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Soon Hak Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Suk Hwan Baek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hyeun Wook Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyeun Wook Chang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moon, T.C., Kim, M.S., Lee, S.J. et al. Detection and characterization of 45 kDa platelet activating factor acetylhydrolase in cerebrospinal fluid of children with meningitis. Arch Pharm Res 26, 554–558 (2003). https://doi.org/10.1007/BF02976880

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02976880

Key words

Search

Navigation