Ar-turmerone and β-atlantone induce internucleosomal DNA fragmentation associated with programmed cell death in human myeloid leukemia HL-60 cells
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In the course of a search for antitumor agents, we found that the extract ofCurcuma longa was effective in inducing apoptosis or programmed cell death (PCD) in human myeloid leukemia cells (HL-60). Active compounds for PCD were isolated from the hexanic extraction of the rhizome ofCurcuma longa. With the several chromatographies, and spectral data, they were identified as ar-turmerone and β-atlantone. The present results demonstrate that the exposure of human myeloid leukemia HL-60 cells to clinically achievable concentrations of arturmerone (TU) or β-atlantone (AT) produced internucleosomal DNA fragmentation of approximately 200 base-pair multiples, and the morphological changes characteristic of cells undergoing apoptosis or PCD. This findings suggest that these agents may exert their antitumoral activity, in part, through induction of apoptosis(PCD).
Key wordsar-Turmerone β-atlantone Anticancer activity Apoptosis Programmed cell death
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