Gram-positive bacteria specific properties of silybin derived fromSilybum marianum


Silybin has a potent antibacterial activity, more potent than silymarin II, against gram-positive bacteria without hemolytic activity, whereas it has no antimicrobial activity against gram-negative bacteria or fungi. The mode of action of silybin against the gram-positive bacterial cell was examined by investigating the change in plasma membrane dynamics of bacterial cells using 1,6-diphenyl-1,3,5-hextriene (DPH) as a membrane probe and by assessing the inhibition of macromolecular synthesis using radiolabeled incorporation assay. The results showed that silybin inhibited RNA and protein synthesis on gram-positive bacteria.

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Correspondence to Dong Gun Lee or Hyung Keun Kim or Yoonkyung Park or Seong-Cheol Park or Eun-Rhan Woo or Hye Gwang Jeong or Kyung-Soo Hahm.

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Lee, D.G., Kim, H.K., Park, Y. et al. Gram-positive bacteria specific properties of silybin derived fromSilybum marianum . Arch Pharm Res 26, 597–600 (2003).

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Key words

  • Silybin
  • Gram-positive bacteria
  • 1,6-Diphenyl-1,3,5-hextriene (DPH)