Abstract
The antioxidant activity ofJuniperus chinensis (Cupressaceae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract ofJ. chinensis heartwood showed the strong antioxidant activity. The antioxidant activity ofn-BuOH soluble fraction was stronger than that of the others, and the fraction was subjected to purification by repeated silica gel and Sephadex LH-20 column chromatography. Quercetin, naringenin, taxifolin, aromadendrin and isoquercitrin were isolated from then-BuOH fraction. Their structures were elucidated by physico-chemical and spectroscopic studies.
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Ali, A. M., Mackeen, M. M., Intan, S., Hamid, M., Lajis, N. H., El-Sharkawv, J. H. and Murakoshi, M., Antitumor-promoting and antitumor activities of the crude extract from the leaves ofJuniperus chinensis.J. Ethnopharmacol., 53, 65–70 (1996).
But, P. P. H., Kimura, T., Gue, J.-X. and Sung, C. K., International collation of traditional and folk medicine. Vol. 2. World scientific publishing Co. Pte. Ltd., Singapore, pp. 16–17 (1997).
Chien, M. M., Svoboda, G. h., Schiff, Jr. P. L., Slatkin, D. J. and Knapp, J. E., Chemical constituents ofEchites hirsuta.J. Pharm. Sci., 68, 247–249 (1979).
Fang, J. M., Lee, C. K. and Cheng, Y. S., Diterpenes from leaves ofJuniperus chinensis.Phytochem., 33, 1169–1172 (1993a).
Fang, J. M., Sou, Y. C. and Chiu, Y. H., Diterpenes from the bark ofJuniperus chinensis.Phytochem., 34, 1581–1584 (1993b).
Fang, J. M., Lee, C. K. and Cheng, Y. S., Lignans from leaves ofJuniperus chinensis.Phytochem., 33, 3659–3661 (1992).
Fang, J. M., Chen, Y. C., Wang, B. W. and Chen, Y. S., Terpenes from heartwood ofJuniperus chinensis.Phytochem., 41, 1361–1365 (1996).
Kawazu, K., Jsmail, I. S., Abdul, M. A. and Kanzaki, H., Isolation of the cytotoxic constituent deoxypodophyllotoxin from the leaves ofJuniperus chinensis.Scientific Reports, 86, 1–6 (1997).
Kim, S. Y., Gao, J. J., Lee, W. C., Ryu, K. S., Lee, K. R. and Kim, Y. C., Antioxidative flavonoids from the leaves ofMorus alba.Arch. Pharm. Res., 22, 81–85 (1999).
Kuo, Y. H. and Chen, W. C., Chinensiol, a new dimeric himachalane-type sesquiterpene from the root ofJuniperus chinensis Linn.Chem. Pharm. Bull., 42, 2187–2189 (1994a).
Kuo, Y. H. and Chen, W. C., Three new diterpenes, 1,3-dioxototarol, isototarolenone, and 1-oxo-3-hydroxytotarol from the roots ofJuniperus chinensis Linn.,Chem. Pharm. Bull., 42, 1774–1776 (1994b).
Kuo, Y. H. and Chen, W. C., 8α-12-Dihydroxycedrane, a new sesquiterpene fromJuniperus chinensis Linn.,J. Chem. Res., 11, 382–383 (1992a).
Kuo, Y. H. and Chen, W. C., 7-Hydroxysandaracopimaric acid. A new diterpene from the root ofJuniperus chinensis.Chemistry Express, 7, 833–837 (1992b).
Kuo, Y. H. and Shin, L. L., Two new sesquiterpenes, 12-hydroxy-α-longipinene and 15-hydroxyacora-4(14),8-diene from the heartwood ofJuniperus chinensis Linn. var.tsukusiensis Masam.Chem. Pharm. Bull., 44, 1758–1760 (1996).
Lee, C. K. and Cheng, Y. S., Diterpenoids from the leaves ofJuniperus chinensis var.kaizuka.J. Nat. Prod., 64, 511–514 (2001).
Lee, C. K., Fang, J. M. and Chen, Y. S., Norditerpenes fromJuniperus chinensis.Phytochem., 39, 391–394 (1995).
Lee, S. M., Chen, W. C., Lai, J. S. and Kuo, Y. H., 12-Hydroxycupressic acid, A new diterpene from the bark ofJuniperus chinensis kaizuca.Chemistry Express, 7, 829–832 (1992).
Lundgren, L. N. and Theander, O.,Cis- andtrans- dihydroquercetin glucosides.Phytochem., 27, 829–832 (1988).
Muranaka, T., Miyata, M., Ito, K. and tachibana, S., Terpenes from heartwood ofJuniperus chinensis.Phytochem., 49, 491–496 (1998).
Reisch, J., Hussain, R. A. and Jester, I., flavonoids fromFlindersia australis.Phytochem., 23, 2114–2115 (1984).
Shen, C. C., Chang, Y. S. and Ho, L. K., Nuclear magnetic resonance studies of 5,7-dihydroxyflavonoids.Phytochem., 34, 843–845 (1993).
Shin, L. L., Chen, W. C. and Kuo, Y. H., Five newcis- himachalane-type sesquiterpenes from the heartwood ofJuniperus chinensis var.tsukusiensis.Chem. Pharm. Bull. 47, 557–560 (1999).
Yoshida, T., Mori, K., Hatano, T., Okumura, T., Uehara, I., Komagoe, K., Fujita, Y. and Okuda, T., Studies on inhibition mechanism of autooxidation by tannins and flavonoids. V. Radical scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical.Chem. Pharm. Bull., 37, 1919–1921 (1989).
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Lim, J.P., Song, Y.C., Kim, J.W. et al. Free radical scavengers from the heartwood ofJuniperus chinensis . Arch Pharm Res 25, 449–452 (2002). https://doi.org/10.1007/BF02976600
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DOI: https://doi.org/10.1007/BF02976600