Antioxidant flavonoids and chlorogenic acid from the leaves ofEriobotrya japonica

  • Hyun Ah Jung
  • Jong Cheol Park
  • Hae Young Chung
  • Jong Kim
  • Jae Sue Choi
Research Articles Natural Products

DOI: 10.1007/BF02976549

Cite this article as:
Jung, H.A., Park, J.C., Chung, H.Y. et al. Arch Pharm Res (1999) 22: 213. doi:10.1007/BF02976549

Abstract

The antioxidant activity ofEriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at 37°C, using 2-thiobarbituric acid (TBA). The methanol extract and its fractions ofEriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc andn-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCI gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside fromn-BuOH fraction, and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and 2α-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

Key words

Eriobotrya japonica Antioxidant activity Chlorogenic acid Flavonoids 

Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Hyun Ah Jung
    • 1
  • Jong Cheol Park
    • 2
  • Hae Young Chung
    • 3
  • Jong Kim
    • 4
  • Jae Sue Choi
    • 1
  1. 1.Department of Food and Life SciencePukyung National UniversityPusanKorea
  2. 2.Department of Oriental Medicine ResourcesSunchon National UniversitySunchonKorea
  3. 3.Department of PharmacyPusan National UniversityPusanKorea
  4. 4.Masan CollegeMasanKoreaKorea

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