Antioxidant flavonoids and chlorogenic acid from the leaves ofEriobotrya japonica
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- Jung, H.A., Park, J.C., Chung, H.Y. et al. Arch Pharm Res (1999) 22: 213. doi:10.1007/BF02976549
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The antioxidant activity ofEriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at 37°C, using 2-thiobarbituric acid (TBA). The methanol extract and its fractions ofEriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc andn-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCI gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside fromn-BuOH fraction, and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and 2α-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.