Abstract
We investigated the optimum conditions for conversion of water-insoluble components of basidiocarps ofGanoderma lucidum to water-soluble components by hydrolyzing with chitinase. We also tried it withGanoderma lucidum residue remaining after extracting hot water-soluble components ofGanoderma lucidum. After hydrolyzing under optimum conditions (20 ppm chitinase, 2%Ganoderma lucidum or 6%Ganoderma lucidum residue, at pH 3 and at 35°C), the contents of total water-soluble components (polysaccharide or protein) were measured, and it was found that the contents of water-soluble components increased to 1.5∼2.7 fold. Michaelis constant, Km and maximum rate, Vmax calculated by Lineweaver-Burk plot for hydrolysis ofGanoderma lucidum were 1.75% and 0.02%/min respectively and those for hydrolysis ofGanoderma lucidum residue were 53.15% and 0.53%/min respectively. The protein-bound polysaccharide was isolated after hydrolysis and molecular weights were measured by Sepharose CL-4B gel filtration and compared with the molecular weights of polysaccharide before hydrolysis.
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References Cited
Austin, P. R., Brine, O. J., Castle, J. E., Zikakis, J. P. Chitin: New facts of research,Science, 212(15) May, 749–753 (1981).
Bradner, W. T., Clarke, D. A. and Stock, C. C. Stimulation of host defence against experimental cancer. I. zymosan and sarcoma 180 in mice,Cancer Res., 18, 347 (1958).
Chihara, G., Hamuro, T., Maeda, Y., Arai, Y. and Fukuoka, F. Antitumor polysaccharide derived chemically from natural glucan (pachyman),Nature, 225, 943 (1970).
Davidson, E. A. Analysis of sugars found in mucopolysaccharides, Methods Enzymol. 8. 52 (1966).
Hackmann, R. H. Studies on chitin. 1. Enzyme degradation of chitin and chitin esters,Austr. J. Biol. Sci., 7, 168–178 (1954).
Hyun, J. W., Choi, E. C., Kim, B. K. Studies on constituents of the higher fungi of Korea(LXVII) Antitumor components of the basidiocarp ofGanoderma lucidum., Kor. J. Mycol., 18(2), 58 (1990).
Kang, C. Y., Shin, M. J., Choi, E. C., Lee, Y. N. and Kim, B. K. Studies on antineoplastic components of Korean basidiomycetes. Mycelial culture and an antineoplastic component ofGanoderma lucidum, Korean Biochem. J., 14, 101 (1980).
Kato, I., Kobayashi, S., Yokokura, T. and Mutai, M. Antitumor activity ofLactobacillus casei in mice.Gann, 72, 517 (1981).
Kim, B. K., Chung, H. S., Chung, K. S. and Yang, M. D. Studies on the antineoplastic components of Korean basidiomycetes,Kor. J. Mycol., 8, 107 (1980).
Knorr, D. Functional properties of chitin and chitosan,J. of Food Sci. 47, 593–595 (1982).
Komoda, Y., Nakamura, H., Ishihara, S., Uchida, M., Kohda, H. and Yamasaki, K. Structures of new terpenoid constituents ofGanoderma lucidum (Fr.) Karst (polyporaceae),Chem. Pharm. Bull. 33(11), 4829–4835 (1985).
Kubota, T., Asaka, Y., Miura, I. and Mori, H. Structure of ganoderic acid A and B, two new lanostane type bitter triterpenes fromGanoderma lucidum Karst (Fr.),Helv. Chim. Acta., 65, 611 (1982).
Lee, K. H., Jeong, H., Kim, Y. I. and Kim, B. K. Production of antihypertensive constituents fromGanoderma lucidum IY005 by fermentation using industrial wastes,Kor. J. Mycol., 19(1), 79 (1990).
Lee, M. H., Kim, H. W., Shim, M. J., Toh, S. H., Choi, E. C. and Kim, B. K., Studies on constituents of the higher fungi of Korea(LVI) General constituents and immuno-stimulation ofGanoderma lucidum, Kor. J. Mycol. 14(2), 149- (1986).
Manuel, E. Y. and Richard, I. B., Kinetics of chitinase production. 1. Chitin hydrolysis,Biotech. and Bioeng. 27 June, 769–775 (1985).
Miyazaki, T. and Nishijima, M. Studies on fungal polysaccharides (XXVII), structural examination of a water-soluble, antitumor polysaccharide ofGanoderma lucidum, Chem. Pharm. Bull., 29, 3611 (1981).
Miyazaski, T. and Nishijima, M. Structural examination of an alkali-extracted, water-soluble heteroglycan of the fungusGanoderma lucidum, Carbohydr. Res., 109, 290 (1982).
Mizuno, T., Suzuki, E., Maki, K. and Tamaki, H. Fractionation, chemical modification and antitumor activity of water-soluble polysaccharides of the fruiting body ofGanoderma lucidum, Nippon Nokeikagaku Kaishi., 59, 1143 (1985).
Nogami, M., Tsuji, Y., Kubo, M., Takahashi, M., Kimura, H. and Matsuike, Y. Studies onGanoderma lucidum V1. Anti-allergic effect(1),Yakugakuzasshi, 106(7), 594–599 (1985).
Nakahara, W., Fukuoka, F., Maeda, Y. and Aoki, K. The host mediated antitumor effect of some plant polysaccharide,Gann, 55, 283 (1964).
Ohtakara, A., Uchida, Y., and Misutomi, M., in Processings of the first international conference on chitin/chitosan, Muzzarelli and Pariser, E., R., Eds. (MIT Sea Grant report MITSG 78-7), 587–600 (1978).
Ringler, R. L., Byerrum, R. U., Steven, T. A., Clark, P. A. and Stock, C. C. Studies on antitumor substances produced by Basidomycetes,Antibiot. Chemother., 7, 1 (1957).
Sannan, T., Kurita, K. and Iwakura, Y., Studies on chitin: 7. IR spectroscopic determination of degree of deacetylation,Polymer, 19, April, 458–459 (1978).
Shin, H. J., Kim, H. W., Choi, E. C. and Kim, B. K. Studies on constituents of the higher fungi of Korea (XLII), inorganic components ofGanoderma lucidum.Kor. J. Mycol., 13, 53 (1985).
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Cheong, JY., Park, WB. Conversion of water-insoluble components of the basidiocarps ofGanoderma lucidum to water-soluble components by hydrolyzing with chitinase. Arch. Pharm. Res. 19, 326–334 (1996). https://doi.org/10.1007/BF02976250
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DOI: https://doi.org/10.1007/BF02976250