Abstract
Perfluorotolyl (PFT)-ether and perfluorotoly-trimethylsilyl (PFT-TMS) ether derivatives of oestrone, 17α- and 17β-oestradiol were prepared under phase transfer conditions. The former derivatives under negative ion chemical ionization conditions gave significant ions in the mass spectrometer but 17α- and 17α-oestradiol gave poor resolution. However, the PFT-TMS derivatives of 17α- and 17β-oestradiol showed good resolution. These derivatives were used for the analysis of oestrogens in bovine aqueous humour, vitreous humour and retina. The mean (±SEM) concentrations of oestrone in bovine aqueous humour (n=18), vitreous humour (n=18) and bovine retina (n=4) were 0.47±0.11, 0.46±0.14 and 1.10±0.24 ng.ml−1, respectively. 17α-Oestradiol was detected in 16 out of 18 samples of bovine aqueous humour and vitreous humour and the mean (±SEM) concentrations were 0.30±0.10 and 0.08±0.02 ng.ml−1, respectively. The mean (±SEM) concentration of 17β-oestradiol in aqueous humour (n=7) and vitreous homour (n=11) 0.83±0.26 ng ml−1 and 0.39±0.09 ng ml−1, respectively. In retina the concentrations of both steroids were below the detection limit.
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Iqbal, Z., Midgley, J.M. & Watson, D.G. Determination of oestrone, 17α- and 17β-oestradiol in bovine aqueous humor using gas chromatography-negative ion chemical ionization mass spectrometry. Arch. Pharm. Res. 20, 247–252 (1997). https://doi.org/10.1007/BF02976152
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DOI: https://doi.org/10.1007/BF02976152