Abstract
CINC-1 is a member of chemokine family with chemotactic and activating properties to neutrophils. CINC-1 induction in IL-1β-stimulated rat fibroblast NRK-49F cells was quantitated by a sensitive ELISA. CINC-1 production was increased up to 135 ng/ml from basal 2–6 ng/ml by stimulation with IL-1β. Steroidal anti-inflammatory drugs including dexamethasone and prednisolone exhibited potent suppressive effects on IL-1β-induced CINC-1 production. Among 39 kinds of natural triterpenoids tested, acacigenin B exhibited the highest suppressive effects with about 10 μM to be 50% of inhibition on the CINC-1 induction. The suppressive potency of acacigenin B on IL-1β-induced CINC-1 production was about 10-fold less than that of the steroidal anti-inflammatory drugs.
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References Cited
Baggiolini, M., Dewald, B. and Walz, A., Interleukin-8 and related chemotactic cytokines, In Gallin, J. I., Goldstein, I. M., and Snyderman, R. (Eds.).Inflammation Basic Principles and Clinical Correlates. Raven Press, New York, pp. 247–264 1992.
Cocchi, F., DeVico, A. L., Garzino-Demo, A., Arya, S. K., Gallo, R. C. and Lusso, P., Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.Science 270, 1811–1815 (1995).
Cramer, E. B., Cell biology of phagocyte migration from the bone marrow, out of the bloodstream, and across organ epithelia, In Gallin, J. I., Goldstein, I. M., and Snyderman, R. (Eds.).Inflammation Basic Principles and Clinical Correlates. Raven Press, New York, pp. 341–351, 1992.
Dragic, T., Litwin, V., Allaway, G. P., Martin, S. R., Huang, Y., Nagashima, K. A., Cyanan, C., Maddon, P. J., Koup, R. A., Moore, J. P. and Paxton, W. A., HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CD-CKR-5.Nature, 381, 667–673 (1996).
D’Souza, M. P. and Harden, V. A., Chemokines and HIV-1 second receptors.Nature Medicine, 2, 1293–1300 (1996).
Feng, Y., Broder, C. C., Kennedy, P. E. and Berger, E. A., HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.Science, 272, 872–877 (1996).
Goldstein, R. A., Bowen, D. L. and Fauci, A. S., Adrenal corticoids, In Gallin, J. I., Goldstein, I. M., and Snyderman, R. (Eds.).Inflammation Basic Principles and Clinical Correlates. Raven Press, New York, pp. 1062–1082, 1992.
Kang, S. S. and Woo, W. S., Sapogenin fromAlbizzia julibrissin.Arch. Pharm. Res., 6, 250–258 (1981).
Lee, G.-I., Ha, J. Y., Min, K. R., Nakagawa, H., Tsurufuji, S., Chang, I.-M., and Kim, Y., Inhibitory effects of oriental herbal medicines on IL-8 induction in lipopolysaccharide-activated rat macrophages.Planta Medica, 61, 26–30 (1995).
Min, B., Lee, G.-I., Ha, J. Y., Min, K. R., Kim, J. S., Kang, S. S. and Kim, Y., Inhibitory effects of triterpenoids on interleukin-8/CINC-1 induction in LPS-stimulated rat peritoneal macrophages.Natural Product Sci., 2, 48–55 (1996).
Mukaida, N., Gussella, G. L., Kasahara, T., Ko, Y., Zachariae, C. O. C., Kawai, T. and Matsushima, K., Molecular analysis of the inhibition of interleukin-8 production by dexamethasone in a human fibrosarcoma cell line.Immunol., 75, 674–679 (1992).
Nakagawa, H., Ikesue, A., Hatakeyama, S., Kato, H., Gotoda, T., Komorita, N., Watanabe, K. and Miyai, H., Production of an interleukin-8-like chemokine by cytokine-stimulated rat NRK-49F fibroblasts and its suppression by anti-inflammatory steroids.Biochem. Pharmacol., 45, 1425–1430 (1993).
Nakagawa, H., Komorita, N., Shibata, F., Ikesue, A., Konishi, K., Fujioka, M. and Kato, H., Identification of cytokine-induced neutrophil chemoattractant (CINC), rat GRO/CINC-2α and CINC-2β produced by granulation tissue in culture: purification, complete amino acid sequence and characterization.Biochem. J., 301, 545–550 (1994).
Schall, T. J., The cytokines, In Thomson, A. W. (Ed.).The Cytokine Handbook. Academic Press, New York, pp. 419–460, 1994.
Shibata, F., Konishi, K., Kato, H., Komorita, N., Al-Mokdad, M., Fujioka, M. and Nakagawa, H., Recombinant production and biological properties of rat cytokine-induced neutrophil chemoattractants, GRO/CINC-2α, CINC-2β and CINC-3.Eur. J. Biochem. 231, 306–311 (1995).
Snyderman, R. and Uhing, R. J., Chemoattractant stimulus-response coupling, In Gallin, J. I., Goldstein, I. M., and Snyderman, R. (Eds.).Inflammation Basic Principles and Clinical Correlates. Raven Press, New York, pp. 421–442, 1992.
Watanabe, K., Koizumi, F., Furashige, Y., Tsurufuji, S. and Nakagawa, H., Rat CINC, a member of the interleukin-8 family, is a neutrophil-specific chemoattractantin vivo.Expt. Mol. Path., 55, 30–37 (1991).
Watanabe, K., Konishi, K., Fujioka, M., Kinoshita, S. and Nakagawa, H., The neutrophil chemoattractant produced by the rat kidney epithelioid cell line NRK-52E is a protein related to the KC/gro protein.J. Biol. Chem., 264, 19559–19563 (1989).
Watanabe, K. and Nakagawa, H., Cytokines enhance the production of a chemotactic factor for polymorphonuclear leukocytes by rat renal glomerular epithelioid cells.Nephron, 54, 169–175 (1990).
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Ha, J.Y., Min, K.R., Kang, SH. et al. Suppressive effects of triterpenoids on CINC-1 induction in interleukin-1β-stimulated rat fibroblast NRK-49F cells. Arch. Pharm. Res. 20, 234–238 (1997). https://doi.org/10.1007/BF02976150
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DOI: https://doi.org/10.1007/BF02976150