Abstract
6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts ofHylomecon hylomeconoides, showed a dose-dependent effect at 1–10 μM on causing apoptotic cell death in HT29 colon carcinoma cells (IC50 = 5.0 ± 0.2 μM). Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.
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Lee, YJ., Yin, HQ., Kim, YH. et al. Apoptosis inducing effects of 6-Methoxydihydrosanguinarine in HT29 colon carcinoma cells. Arch Pharm Res 27, 1253–1257 (2004). https://doi.org/10.1007/BF02975890
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DOI: https://doi.org/10.1007/BF02975890