Abstract
Nucleotide sequence extending 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (pcbC) and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase gene (pcbD) ofPseudomonas sp. DJ-12 was previously analyzed and the two genes were present in the order ofpcbD-pcbC preceded by a promoter fromPseudomonas sp. DJ-12. In this study, a 3.8-kb nucleotide sequence located downstream of thepcbC gene was analyzed to have three open reading frames (ORFs) that are designated asorf1, pcbE andorf2 genes. All of the ORFs were preceded by each ribosome-binding sequence of 5-GGAXA-3 (X=G or A). However, no promoter-like sequence and transcription terminator sequence were found in the analyzed region, downstream ofpcbC gene. Therefore, the gene cluster appeared to be present in the order ofpcbD-pcbC-orf1-pcbE-orf2 as an operon, which is unique organization characterized so far in biphenyl- and PCB-degrading bacteria. Theorf1 gene was composed of 1,224 base pairs which can encode a polypeptide of molecular weight 44,950 containing 405 amino acid residues. A deduced amino acid sequence of theorf1 gene product exhibited 21–33% identity with those of indole dioxygenase and phenol hydroxylase components. ThepcbE gene was composed of 783 base pairs encoding 2-hydroxypenta-2,4-dienoate hydratase involved in the 4-chlorobiphenyl catabolism. Theorf2 gene was composed of 1,017 base pairs encoding a polypeptide of molecular weight 37,378 containing 338 amino acid residues. A deduced amino acid sequence of theorf2 gene product exhibited 31% identity with that of a nitrilotriacetate monooxygenase component.
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Lim, JC., Lee, J., Jang, J.D. et al. Characterization of thepcbE gene encoding 2-hydroxypenta-2,4-dienoate hydratase inPseudomonas sp. DJ-12. Arch Pharm Res 23, 187–195 (2000). https://doi.org/10.1007/BF02975512
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DOI: https://doi.org/10.1007/BF02975512