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Isolation and characterization of proteoglycan derived from human placenta and its biological activities

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Abstract

Chondroitin sulfates proteoglycans were isolated from human placenta. For the identification of enzymatic digestion products of isolated proteoglycan, strong anion exchange-high performance liquid chromatography (SAX-HPLC) was performed. By the action of chondroitin ABC and chondroitin B lyase, three unsaturated disaccharides 2-acetamide-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-D-galactose (ΔDi-OS), 2-acetamide-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose (ΔDi-6S) and 2-acetamide-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose (ΔDi-4S) were produced from the human placenta proteoglycan. The anticoagulant activity of chondroitin sulfate proteoglycan was evaluated by activated partial thromboplastin time (aPTT) assay and thrombin time (TT) assay. The clotting times of aPTT and TT were increased from 72 to 144 sec and 19 to 27 sec, respectively. The immuno-modulating activity of chondroitin sulfate proteoglycan was examined by cell proliferation assay and these results suggest that it may play a role in suppression of the function of immune-related cells.

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, Konyang University, 320-711, Nonsan, Chungnam, Korea

    Kyung Bok Lee & Sang Tae Kwak

  2. Department of Chemistry, Konyang University, 320-711, Nonsan, Chungnam, Korea

    Jong Sig Kim

  3. Department of Microbiology, College of Medicine, Konyang University, 320-711, Nonsan, Chungnam, Korea

    Yung Choon Yoo

  4. Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University, 1370, Sankyuk-Dong, 702-701, Taegu, Korea

    Kyung Sik Song

  5. Natural Products Research Institute, Seoul National University, 110-460, Seoul, Korea

    Yeong Shik Kim

Authors
  1. Kyung Bok Lee
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  2. Jong Sig Kim
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  3. Yung Choon Yoo
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  4. Sang Tae Kwak
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  5. Kyung Sik Song
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Correspondence to Kyung Bok Lee.

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Lee, K.B., Kim, J.S., Yoo, Y.C. et al. Isolation and characterization of proteoglycan derived from human placenta and its biological activities. Arch Pharm Res 23, 182–186 (2000). https://doi.org/10.1007/BF02975511

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  • DOI: https://doi.org/10.1007/BF02975511

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