For an attempt to develop safe materials protecting solar ultraviolet (UV)-induced skin damage, plant extracts were evaluated for their inhibitory activities of free radical generation and arachidonic acid/metabolites release from UVB-irradiated normal human keratinocytes. From the results of these screening procedures, the ethanol extract of the flowers ofPrunus persica (Ku-35) was selected for further study. It was found that Ku-35 (100-1,000 μg/ml) inhibited the amount of14C-arachidonic acid/metabolites release from UVB-irradiated keratinocytes. It was also demonstrated that Ku-35 possessed the protective activity against UV-induced cytotoxicity of keratinocytes and fibroblasts. In addition, Ku-35 was revealed to protect UVB-induced erythema formation using guinea pigs in preliminaryin vivo study. All these results indicate that the flowers ofP. persica extract may be beneficial for protecting UV-induced skin damage when topically applied.
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Boyces, S. T. and Ham, R. G., Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum free serial culture.J. Invest. Dermatol., 81 (Suppl.), 33S-40S (1983).
Chen, X., Gresham, A., Morrison, A. and Pentland, A. P., Oxidative stress mediates synthesis of phospholipase A2 after UVB injury.Biochim. Biophys. Acta, 1299, 23–33 (1996).
Darr, D., Combs, S., Dunston, S., Manning, T. and Pinnell, S., Topical vitamin C protects porcine skin from ultraviolet-induced damage.Br. J. Dermatol., 127, 247–253 (1992).
Englaro, W., Derijard, B., Ortonne, J-P. and Ballotti, R., Solar ultraviolet light activates extracellular signal-regulated kinases and the ternary complex factor in human normal keratinocytes.Oncogene, 16, 661–664 (1998).
Isoherranen, K., Punnonen, K., Jansen, C and Uotila, P., Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes.Br. J. Dermatol., 140, 1017–1022 (1999).
Kang-Rotondo, C. H., Miller, C. C., Morrison, A. R. and Pentland, A. P., Enhanced keratinocyte prostaglandin synthesis after UV injury is due to increased phospholipase activity.Am. J. Physiol., 264, C396-C401 (1993).
Kim, H. P., Indu, M., Iversen, L. and Ziboh, V. A., Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guineapigs.Prostg. Leukot. Essen. Fatty Acids, 58, 17–24 (1998).
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays.J. Immunol. Methods, 65, 55–63 (1983).
Streilein, J. W., UVB susceptibility: Possible relationship to photoaging and Photocarcinogenesis, In Gilchrest, B. A. (Ed.).Photoaging. Cambridge, New York, pp. 68–80, 1995.
Tebbe, B., Wu, S., Geilen, C. C., Erberle, J., Kodelja, V. and Orfanos, C. E., L-Ascorbic acid inhibits UVA-induced lipid peroxidation and secretion of IL-1a and IL-6 in cultured human keratinocytesin vitro.J. Invest. Dermatol., 108, 302–306 (1997).
Thiele, J. J., Traber, M. G. and Packer, L., Depletion of human stratum corneum vitamin E: an early and sensitivein vivo marker of UV induced photo-oxidation.J. Invest. Dermatol., 110, 756–761 (1998).
Urbach, F., Potential effects of altered solar ultraviolet radiation on human skin cancer.Photochem. Photobiol., 50, 507–513 (1989).
Weber, C., Podda, M., Rallis, M., Thiele, J. J., Traber, M. G. and Packer, L., Efficacy of topically applied tocopherols and tocotrienols in protection of murine skin from oxidative damage induced by UV-irradiation.Free Rad. Biol. Med., 22, 761–769 (1997).
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Kim, Y.H., Yang, H.E., Kim, J.H. et al. Protection of the flowers ofPrunus persica extract from ultraviolet B-induced damage of normal human keratinocytes. Arch Pharm Res 23, 396–400 (2000). https://doi.org/10.1007/BF02975454