Skip to main content
SpringerLink
Log in

Protection of the flowers ofPrunus persica extract from ultraviolet B-induced damage of normal human keratinocytes

  • Research Articles
  • Biomedical Sciences
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

For an attempt to develop safe materials protecting solar ultraviolet (UV)-induced skin damage, plant extracts were evaluated for their inhibitory activities of free radical generation and arachidonic acid/metabolites release from UVB-irradiated normal human keratinocytes. From the results of these screening procedures, the ethanol extract of the flowers ofPrunus persica (Ku-35) was selected for further study. It was found that Ku-35 (100-1,000 μg/ml) inhibited the amount of14C-arachidonic acid/metabolites release from UVB-irradiated keratinocytes. It was also demonstrated that Ku-35 possessed the protective activity against UV-induced cytotoxicity of keratinocytes and fibroblasts. In addition, Ku-35 was revealed to protect UVB-induced erythema formation using guinea pigs in preliminaryin vivo study. All these results indicate that the flowers ofP. persica extract may be beneficial for protecting UV-induced skin damage when topically applied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boyces, S. T. and Ham, R. G., Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum free serial culture.J. Invest. Dermatol., 81 (Suppl.), 33S-40S (1983).

    Article  Google Scholar 

  • Chen, X., Gresham, A., Morrison, A. and Pentland, A. P., Oxidative stress mediates synthesis of phospholipase A2 after UVB injury.Biochim. Biophys. Acta, 1299, 23–33 (1996).

    PubMed  Google Scholar 

  • Darr, D., Combs, S., Dunston, S., Manning, T. and Pinnell, S., Topical vitamin C protects porcine skin from ultraviolet-induced damage.Br. J. Dermatol., 127, 247–253 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Englaro, W., Derijard, B., Ortonne, J-P. and Ballotti, R., Solar ultraviolet light activates extracellular signal-regulated kinases and the ternary complex factor in human normal keratinocytes.Oncogene, 16, 661–664 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Isoherranen, K., Punnonen, K., Jansen, C and Uotila, P., Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes.Br. J. Dermatol., 140, 1017–1022 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Kang-Rotondo, C. H., Miller, C. C., Morrison, A. R. and Pentland, A. P., Enhanced keratinocyte prostaglandin synthesis after UV injury is due to increased phospholipase activity.Am. J. Physiol., 264, C396-C401 (1993).

    PubMed  CAS  Google Scholar 

  • Kim, H. P., Indu, M., Iversen, L. and Ziboh, V. A., Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guineapigs.Prostg. Leukot. Essen. Fatty Acids, 58, 17–24 (1998).

    Article  CAS  Google Scholar 

  • Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays.J. Immunol. Methods, 65, 55–63 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Streilein, J. W., UVB susceptibility: Possible relationship to photoaging and Photocarcinogenesis, In Gilchrest, B. A. (Ed.).Photoaging. Cambridge, New York, pp. 68–80, 1995.

  • Tebbe, B., Wu, S., Geilen, C. C., Erberle, J., Kodelja, V. and Orfanos, C. E., L-Ascorbic acid inhibits UVA-induced lipid peroxidation and secretion of IL-1a and IL-6 in cultured human keratinocytesin vitro.J. Invest. Dermatol., 108, 302–306 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Thiele, J. J., Traber, M. G. and Packer, L., Depletion of human stratum corneum vitamin E: an early and sensitivein vivo marker of UV induced photo-oxidation.J. Invest. Dermatol., 110, 756–761 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Urbach, F., Potential effects of altered solar ultraviolet radiation on human skin cancer.Photochem. Photobiol., 50, 507–513 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Weber, C., Podda, M., Rallis, M., Thiele, J. J., Traber, M. G. and Packer, L., Efficacy of topically applied tocopherols and tocotrienols in protection of murine skin from oxidative damage induced by UV-irradiation.Free Rad. Biol. Med., 22, 761–769 (1997).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Kangwon National University, 200-701, Chunchon

    Young Ha Kim, Hye Eun Yang, Moon Young Heo & Hyun Pyo Kim

  2. R & D Center, Coreana Cosmetics Co Ltd., 333-830, Cheonan, Korea

    Jong Ha Kim

Authors
  1. Young Ha Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hye Eun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jong Ha Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Moon Young Heo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyun Pyo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyun Pyo Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, Y.H., Yang, H.E., Kim, J.H. et al. Protection of the flowers ofPrunus persica extract from ultraviolet B-induced damage of normal human keratinocytes. Arch Pharm Res 23, 396–400 (2000). https://doi.org/10.1007/BF02975454

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02975454

Key words

Search

Navigation