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Determination of the kinetic properties of platycodin D for the inhibition of pancreatic lipase using a 1,2-diglyceride-based colorimetric assay

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Abstract

A 1, 2-diglyceride-based multi-step colorimetric assay to measure the pancreatic lipase activity was applied for the determination of the kinetic profiles of the lipase inhibition with a slight modification and the validity verification. With this assay method, our study revealed that platycodin D, one of major constituents of Platycodi Radix, inhibits the pancreatic lipase activity in a competitive type, with the value ofK l being 0.18±0.02 mM. In addition, PD has affected the values ofK m, app andK cat/K m in a dose- dependent manner. The results shed a meaningful light on how PD mediates lipid metabolism in the intestinal tracts. On the other hand, since the revised assay is sensitive, rapid, and does not affect the accuracy to the kinetic properties, it is applicable not only to evaluation of the kinetic properties of the pancreatic lipase, but also to highthroughput screening of pancreatic lipase activity.

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References

  • Burdette, R. A. and Quinn, D. M., Interfacial reaction dynamics and acyl-enzyme mechanism for lipoprotein lipase-catalyzed hydrolysis of lipidp-nitrophenyl esters.J. Biol. Chem., 261, 12016–12021 (1986).

    PubMed  CAS  Google Scholar 

  • Chung, J., Continuous pH-stat titration method for the assay of lipoprotein lipase activityin vitro.Anal. Biochem., 62, 134–148 (1974).

    Article  PubMed  CAS  Google Scholar 

  • David, L. N., Lehninger Principles of biochemistry. 3rd ed. Worth Publishers. New York. pp. 263–265 (2000).

    Google Scholar 

  • Drent, M. L. and Van der Veen, E. A., Lipase inhibition, a novel concept in the treatment of obesity.Int. J. Obes. Relat. Metab. Disord., 17, 241–244 (1993).

    PubMed  CAS  Google Scholar 

  • Farrigan, C. and Pang, K., Obesity market overview.Nat. Rev. Drug Discov., 1, 257–258 (2000).

    Article  Google Scholar 

  • Han, L. K., Xu, B. J., Kimura, Y., Zheng, Y., and Okuda, H., Platycodi Radix affects lipid metabolism in mice with high fat diet-induced obesity.J. Nutr., 130, 2760–2764 (2000).

    PubMed  CAS  Google Scholar 

  • Han, L. K., Zheng, Y. N., Xu, B. J., Okuda, H., and Kimura, Y., Saponins from Platycodi Radix ameliorate high fat dietinduced obesity in mice.J. Nutr., 132, 2241–2245 (2002).

    PubMed  CAS  Google Scholar 

  • Han, L. K., Kimura, Y., Kawashima, M., Takaku, T., Taniyama, T., Hayashi, T., Zheng, Y. N., and Okuda, H., Anti-obesity effects in rodents of dietary teasaponin, a lipase inhibitor.Int. J. Obes. Relat. Metab. Disord., 25, 1459–1464 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Hiroahi, I. and Yohko, Y., Saponins from the roots ofPlatycodon grandiflorum. Part 1. structure of prosapogenins.J. Chem. Soc. Perkin Trans I, 1928–1933 (1981).

  • Imamura, S., Arai, T., Takao, K., and Misaki, H., An enzymatic method using 1,2-diglyceride for pancreatic lipase test in serum.Clin. Chem., 35, 1126 (1989).

    Google Scholar 

  • Keating, G. M. and Jarvis, B., Orlistat: in the prevention and treatment of type 2 diabetes mellitus.Drugs, 61, 2107–2119; discussion 2120-2121 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Panteghini, M., Bonora, R., and Pagani, F., Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate.Ann. Clin. Biochem., 38, 365–370 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Pencreac’h, G., Graille, J., Pina, M., and Verger, R., An ultraviolet spectrophotometric assay for measuring lipase activity using long-chain triacyglycerols from Aleurites fordii seeds.Anal. Biochem., 303, 17–24 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Ros, E., Intestinal absorption of triglyceride and cholesterol. Dietary and pharmacological inhibition to reduce cardiovascular risk.Atherosclerosis, 151, 357–379 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Tietz, N. W., Astles, J. R., and Shuey, D. R., Lipase activity measured in serum by a continuous-monitoring pH-Stat technique-an update.Clin. Chem., 35, 1688–1693 (1989).

    PubMed  CAS  Google Scholar 

  • Uusitupa, M., New aspects in the management of obesity, operation and the impact of lipase inhibitors.Curr. Opin. Lipidol., 10, 3–7 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Wilding, J., The future of obesity treatment.Exs., 89, 181–191 (2000).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Natural Products Research Institute, College of Pharmacy, Seoul National University, 28 Yeonkun-Dong, 110-460, Jongno-Ku, Seoul, Korea

    Hai Lin Zhao & Yeong Shik Kim

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  1. Hai Lin Zhao
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  2. Yeong Shik Kim
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Correspondence to Yeong Shik Kim.

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Zhao, H.L., Kim, Y.S. Determination of the kinetic properties of platycodin D for the inhibition of pancreatic lipase using a 1,2-diglyceride-based colorimetric assay. Arch Pharm Res 27, 1048–1052 (2004). https://doi.org/10.1007/BF02975430

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  • DOI: https://doi.org/10.1007/BF02975430

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