The hepatoprotective effect of DA-9601, a quality-controlled extract ofArtemisia asiatica, on liver damage induced by acetaminophen (APAP) and carbon tetrachloride (CCI4) was investigated by means of serum-biochemical, hepatic-biochemical, and histopathological examinations. Doses of DA-9601 (10, 30, or 100mg/kg) were administered intragastrically to each rat on three consecutive days i.e. 48 h, 24 h and 2 h before a single administration of APAP (640 mg/kg, i.p.) or CCl4 (2 ml/kg, p.o.). Four h and 24 h after hepatotoxin treatment, the animals were sacrificed for evaluation of liver damage. Pretreatment of DA-9601 reduced the elevation of serum ALT, AST, LDH and histopathological changes such as centrilobular necrosis, vacuolar degeneration and inflammatory cell infiltration dose-dependently. DA-9601 also prevented APAP- and CCl4-induced hepatic glutathione (GSH) depletion and CCl4-induced increase of hepatic malondialdehyde (MDA), a parameter of lipid peroxidation, in a dose-dependent manner. These findings suggest that pretreatment with DA-9601 may reduce chemically induced liver injury by complex mechanisms which involve prevention of lipid peroxidation and preservation of hepatic GSH.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Ahn, B.-O., Ryu, B.-K., Ko, J.-I., Oh, T.-Y., Kim, S.-H., Kim, W.-B., Yang, J., Lee, E.-B. and Hahm, K.-B., Beneficial effect of DA-9601, an extract ofArtemisiae Herba, on animal models of inflammatory bowel disease.J. Appl. Pharmacol., 5, 165–173 (1997).
Chenoweth, M. B. and Hake, C. L., The smaller balogenated aliphatic hydrocarbons.Annu. Rev. Pharmacol., 2, 363–398 (1963).
Gilani, A. H. and Janbez, K. H., Prevention and curative effects ofArtemisia absinthium on acetaminophen and CCl4-induced hepatotoxicity.Gen. Pharmacol., 26, 309–315 (1995).
Gilani, A. H., Janbez, K. H., Lateef, A. and Zaman, M., Ca+2 channel blocking activity ofArtemisia scoparia extract.Phytotherapy Res., 8, 161–165 (1994).
Griffith, O. W., Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine.Anal. Biochem., 106, 207–212 (1980).
Janbaz, K. H. and Gilani, A. H., Evaluation of the protective potential ofArtermisia maritima extract on acetaminophen-and CCl4-induced liver damage.J. Ethnopharmacol., 47, 43–47 (1995).
Kim, O.-J., Kang, K.-K., Kim, D.-H., Baik, N.-G., Ahn, B.-O., Kim, W.-B. and Yang, J., Four-week oral toxicity study of DA-9601, an antiulcer agent of Artemisia spp. extract, in rats.J. Appl. Pharmacol., 4, 354–363 (1996).
Krawisz, J. E., Sharon, P. and Stenson, W. F., Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models.Gastroenterology, 87, 1344–1350 (1984).
Lee, E.-B., Cheon, S.-A., Le, E.-S., Kim, O.-K., Ko, S.-T., Yu, K.-J., Shin, D.-S., Kang, S.-Y., Kim, S.-H. and Sohn, M.-H., General pharmacology of Artemisia extract powder, DA-9601.J Appl. Pharmacol., 4, 174–183 (1996).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the folin phenol reagent.J. Biol. Chem., 193, 265–275 (1951).
Nelson, E. B., Montes, M. and Goldstein, M., Effectiveness of metyrapone in the treatment of acetaminophen toxicity in mice.Toxicology, 17, 73–81 (1980).
Oh, T.-Y., Ryu, B.-K., Park, J.-B., Lee, S.-D., Kim, W.-B., Yang, J. and Lee, E.-B., Studies on antiulcer effects of DA-9601, anArtemisiae Herba extract against experimental gastric ulcers and its mechanism.J. Appl. Pharmacol., 4, 111–121 (1996).
Oh, T.-Y., Ahn, B.-O., Ko, J.-I., Ryu, B.-K., Son, M.-W., Kim, S.-H., Kim, W.-B. and Lee, E.-B., Studies on protective effect of DA-9601, anArtemisiae Herba extract, against ethanol-induced gastric mucosal damage and its mechanism.J. Appl. Pharmacol., 5, 202–210 (1997a).
Oh, T.-Y., Ryu, B.-K., Ko, J.-I., Ahn, B.-O., Kim, S.-H., Kim, W.-B., Lee, E.-B., Jin, J.-H. and Hahm, K.-B., Protective effect of DA-9601, an extract ofArtemisiae Herba, against naproxen-induced gastric damage in arthritic rats.Arch. Pharm. Res., 20, 414–419 (1997b).
Ohkawa, H., Ohishi, N. and Yagi, K., Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction.Anal. Biochem., 95, 351–358 (1979).
Plaa, G. L. and Hewitt, W. R., Quantative evaluation of indicies of hepatotoxicity, In Zakim, D. and Boyer, T. D. (Eds.).Toxicology of the Liver. Raven Press New York, pp. 103–120, 1982.
Recknagel, R. O. and Glende, E. A., Carbontetrachloride hepatoxicity: an example of lethal cleavage.CRC. Crit. Rev. Toxicol., 2, 263–297 (1973).
Sallie, R., Tredgeri, J. and William, R., Drugs and the liver.Biopharmaceut. Drug Dispos., 12, 251–259 (1991).
Yang, J., DA-9601, an Artemisiae extract of antiulcer agent. Final report of '95 Good Health R&D Program, Ministry of Health and Welfare, Republic of Korea (1995).
About this article
Cite this article
Ryu, B.K., Ahn, B.O., Oh, T.Y. et al. Studies on protective effect of DA-9601,Artemisia asiatica extract, on acetaminophen- and CCI4-induced liver damage in rats. Arch. Pharm. Res. 21, 508–513 (1998). https://doi.org/10.1007/BF02975366
- Artemisia asiatica
- Carbon tetrachloride