Abstract
This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations ofRubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-Müllers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller’s method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves ofR. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment ofRubus L. species leaves.
References
Alice, L. A. and Campbell, Ch. S., Phytogeny ofRubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences.Am. J. Bot., 86, 81 -97 (1999).
Amakura, Y., Okada, M., Tsuji, S., and Tonogai, Y., High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits.J. Chromatogr. A, 896, 87–93 (2000).
Barch, D. H., Rundhaugen, L. M., Stoner, G. D., Pillay, N. S., and Rosche, W. A., Structure-function relationships of the dietary anticarcinogen ellagic acid.Carcinogenesis, 17, 265–269 (1996).
Borkowski, B., Lutomski, J., Skrzydlewska, E., and Zygmunt, B., Rosliny lecznicze w fitoterapii, IRiPZ, Poznan pp. 470–471 (1994).
Burn, J. H. and Withell, E. R., A principle in raspberry leaves which relaxes uterine muscle.Lancet, 5, 1–3 (1941).
Christ, B. and Muller, K. H., Determination of the amount of flavonol derivatives in drugs.Arch. Pharm., 293, 1033–1042 (1960).
Czygan, F. Ch., Die Himbeere-Rubus idaeus L. Portrait einer Arzneipflanze.Z Phytother., 16, 366–374 (1995).
DAB 10 Deutsches Arzeneibuch, Amtliche Ausgabe, Deutcher Apotheker Verlag, Stuttgart p. 197 (1998).
Daniel, E. M., Krupnick, A. S., Heur, Y. H., Blinzler, J. A., Nims, R. W., and Stoner, G. D., Extraction, stability and quantitation of ellagic acid in various fruits and nuts.J. Food Comp. Anal., 2, 338–349 (1989).
Gudej, J., Kaempferol and quercetin glycosides fromRubus idaeus L leaves.Acta Polon. Pharm., 60, 313–316 (2003).
Gudej, J. and Rychlinska, I., Flavonoid compounds from the leaves ofRubus idaeus L.Herba Pol., 42, 257–261 (1996).
Gudej, J., Tomczyk, M., Urban, E., and Tomczykowa, M., Analysis of chemical composition ofRubus saxatilis L. leaves.Herba Pol., 44, 340–344 (1998).
Gwozdecki, J., Zeszyty pomologiczne, and Malina i Jezyna. Instytutu Sadownictwa i Kwiaciarstwa, Skierniewice pp. 5–53 (1996).
Häkkinen, S., Heinonen, M., Kärenlampi, S., Mykkänen, H., Ruuskanen, J., and Törrönen, R., Screening of selected flavonoids and phenolic acids in 19 berries.Food Res. Int., 32, 345–353 (1999).
Häkkinen, S. H., Kärenlampi, S. O., Mykkänen, H. M., Heinonen, I. M., and Törrönen, A. R., Ellagic acid content in berries: influence of domestic processing and storage.Eur. Food Res. Technol., 212, 75–80 (2000).
Havsteen, B., The biochemistry and medical significance of flavonoids.Pharmacol. Therap., 96, 67–202 (2002).
Heslop-Harrison, Y.,Rubus L. In: Tutin TGet al. (eds.)Flora Europaea. Vol. II, Cambridge University Press p. 7–25 (1968).
Krawczyk, A., Thiem, B., and Szkudlarek, M., High-performance liquid chromatography of ellagic acid from the leaves ofRubus chamaemorus L.Chem. Anal. (Warsaw), 48, 891–899 (2003).
Krzaczek, T., Phenolic acids in some tannin drugs of the Rosaceae family.Farm. Pol., 40, 475–477 (1984).
Marczal, G., Qualitative studies of the tannin content ofRubus idaeus leaf.Herb. Hung., 2, 347–357 (1963).
Mullen, W., McGinn, J., Lean, M. E. J., MacLean, M. R., Gardner, P., Duthie, G. G., Yokota, T., and Crozier, A., Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties.J. Agric. Food Chem., 50, 5191 -5196 (2002).
Okuda, T., Yoshida, T., Hatano, T., Iwasaki, M., Kubo, M., Orime, T., Yoshizaki, M., and Naruhashi, N., Hydrolysable tannins as chemotaxonomic markers in the Rosaceae.Phytochemistry, 31, 3091–3096 (1992).
Olszewska, M., Glowacki, R., Wolbioe, M., and Bald, E., Quantitative determination of flavonoids in the flowers and leaves ofPrunus spinosa L.Acta Polon. Pharm., 58, 199–203 (2001).
Ozarowski, A., and Jaroniewski, W., Rooeliny lecznicze i ich praktyczne zastosowanie, IWZZ, Warszawa pp. 184, 243 (1989).
Patel, A. V., Rojas-Vera, J., and Dacke, C. G., Therapeutic constituents and actions ofRubus species.Curr. Med. Chem., 11, 1501–1512 (2004).
Polish Pharmacopoeia VI, Polish Pharmaceutical Society (eds.), Warszawa p. 150 (2002).
Priyadarsini, K. I., Khopde, S. M., Kumar, S. S., and Mohan, H., Free radical studies of ellagic acid, a natural phenolic antioxidant.J. Agric. Food Chem., 50, 2200–2206 (2002).
Robbers, J. E., and Tyler, V. E., In: Tyler’s Herbs of choice: the therapeutic use of phytomedicinals, The Haworth Herbal Press, New York-London pp. 63–64, 194-195 (1999).
Rojas-Vera, J., Patel, A. V., and Dacke, C. G., Relaxant activity of raspberry (Rubus idaeus) leaf extract in guinea-pig ileumin vitro.Phytother. Res., 16, 665–668 (2002).
Rommel, A. and Wrolstad, R. E., Ellagic acid content of red raspberry juice as influenced by cultivar, processing and environmental factors.J. Agric. Food Chem., 41, 1951–1960 (1993).
Shepherd, T., Robertson, G. W., Griffiths, D. W., and Birch, A. N. E., Epicuticular wax ester and triacylglycerol composition in relation to aphid infestation and resistance in red raspberry (Rubus idaeus L).Phytochemistry, 50, 1255–1267 (1999).
Simpson, M., Parsons, M., Greenwood, J., and Wade, K., Raspberry leaf in pregnancy: its safety and efficacy in labor.J. Midwifery Womens Health, 46, 51–59 (2001).
Sticher, O., Quality ofGinkgo preparations.Planta Med., 59, 2–11 (1993).
Thiem, B. and Gooeliñska, O., Antimicrobial activity ofRubus chamaemorus leaves.Fitoterapia, 75, 93–95 (2004).
Tomczyk, M. and Gudej, J., Quantitative analysis of flavonoids in the flowers and leaves ofFicaria verna Huds.Z. Naturforsch., 58c, 762–764 (2003).
Wójcik, E., Phytochemical investigation ofRubus plicatus blackberry.Acta Polon. Pharm., 46, 386–390 (1989).
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Gudej, J., Tomczyk, M. Determination of Flavonoids, Tannins and Ellagic acid in leaves fromRubus L. species. Arch Pharm Res 27, 1114–1119 (2004). https://doi.org/10.1007/BF02975114
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DOI: https://doi.org/10.1007/BF02975114