Abstract
Enantiomers of five racemic β2-agonists were investigated by capillary electrophoresis employing a hydroxypropyl-β-cyclodextrin (HP-β-CD). The effects of the concentration of HP-β-CD added to the background electrolyte and of the pH of the buffer on the effective mobility and resolution of the studied compounds were examined. Very good resolution was achieved for terbutaline and clenbuterol; salbutamol and bambuterol was able to be partially resolved. Enantioselectivity and resolution were influenced by the concentration of the HP-β-CD, buffer composition and pH.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahuja, S. and Ashman, J., Analytical profiles of drug substances: Terbutaline sulfate, Ciba-Ceigy Corp., New York, 19, 601–625 (1990).
Ariens, E. J., Drug Design, Academic press, London, p 77 (1971).
Aumatell, A., Wells, R. J., and Wong, D. K. Y., Enantiomeric differentiation of a wide range of pharmacologically active substances by capillary electrophoresis using modified β-cyclodextrins.J. Chromatogr. A, 686, 293–307 (1994).
Bechet, I., Paque, P., Fillet, M., Hubert, P., and Crommen, J., Chiral separation of basic drugs by capillary zone electrophoresis with cyclodextrin additives.Electrophoresis, 15, 818–823 (1994).
Desiderio, C. and Fanali, S., Use of negatively charged sulfobutyl ether-β-cyclodextrin for enantiomeric separation by capillary electrophoresis.J. Chromatogr. A, 716, 183–196 (1995).
Fanali, S., Flieger, M., Steinerova, N., and Nardi, A., Use of Cyclodextrins for the Enantioselective separation of ergot alkaloids by capillary zone electorphoresis.Electrophoresis, 13, 39–43 (1992).
Gausepohl, C. and Blaschke, C., Stereoselective determination of clenbuterol in human urine by capillary electrophoresis.J. Chromatogr. B, 713, 443–446 (1998).
Ingelse, B. A., Sarmini, K., Reijenga, J. C., Kenndler, E., and Everaerts, F. M., Chiral interactions in capillary zone electrophoresis: computer simulation and comparison with experiment.Electrophoresis, 18, 938–42 (1997).
Joyce, K. B., Jones, A. E., Scott, R. J., Biddlecombe, R. A., and Pleasance, S., Determination of the enantiomers of salbutamol and its 4-O-sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry.Rapid Commun. Mass Spectrom., 12, 1899–1910 (1998).
Kim, K. H., Kim, H. J., Homg, S. P., and Shin, S. D., Determination of terbutaline enantiomers in human plasma by coupled achiral-chiral high performance liquid chromatography.Arch. Pharm. Res., 23, 441–445 (2000).
Palmarsdottir, S. and Edholm, L. E., Capillary zone electrophoresis for separation of drug enantiomers using cyclodextrins as chiral selectors.J. Chromatogr. A, 666, 337–350 (1994).
Terabe, S., Otsuka, K., and Nishi, H., Separation of enantiomers by CE techniques.J. Chromatogr. A, 666, 295–319 (1994).
Walhagen, A. and Edholm, L. E., Coupled-column chromatography on immobilized protein phases for direct separation and determination of drug enantiomers in plasma.J. Chromatogr., 472, 371–379 (1989).
Wang, Z., Sun, Y., and Sun, Z., Enantiomeric separation of amphetamine and phenylephrine by cyclodextrin-mediated capillary zone electrophoresis.J. Chromatogr. A, 735, 295–301 (1996).
Wren, S. A. C. and Rowe, R. C., Theoretical aspects of chiral separation in capillary electrophoresis.J. Chromatogr. 635, 113–118 (1993).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, K.H., Kim, H.J., Jeun, E.Y. et al. Chiral separation of β2-agonists by capillary electrophoresis using hydroxypropyl-α-cyclodextrin as a chiral selector. Arch Pharm Res 24, 281–285 (2001). https://doi.org/10.1007/BF02975092
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02975092